Inositol-containing Ceratonia siliqua Linn extract
The technology of extract and carob bean is applied in the field of carob bean extract and preparation thereof, and can solve problems such as immaturity of inositol extraction method
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Embodiment 1
[0017] Example 1 Preparation of Carob Extract Containing Inositol
[0018] 1) Weigh 100g of carob, wash and dry;
[0019] 2) Put carob powder in a round-bottomed flask, add 2L of distilled water, extract at 100°C for 3 hours, centrifuge, add 1.5L of distilled water to the filter residue for extraction once, centrifuge to discard the residue, combine the filtrate twice, and concentrate under reduced pressure to the original volume 1 / 30 of;
[0020] 3) Add pH 4.5 acetic acid buffer solution and 2.5% phytase concentrate, enzymolyze at 40°C for 3 hours, concentrate under reduced pressure to 1 / 5 volume, remove acetic acid, adjust pH to neutral, and obtain enzymatic hydrolysis solution ;
[0021] 4) The above enzymatic solution was subjected to column chromatography through HZ202 type anion exchange resin, the resin was washed with 500mL deionized water, the eluate was collected, the eluate was concentrated under reduced pressure, and dried at 105°C to obtain carob beans containi...
Embodiment 2
[0022] Example 2 Preparation of Carob Extract Containing Inositols
[0023] 1) Weigh 100g of carob, wash, dry, put in a grinder and grind into powder;
[0024] 2) Put the dried carob in a round bottom flask, add 2L of 80% ethanol solution, extract at 80°C for 3 hours, centrifuge, add 1.5L of 80% ethanol solution to the filter residue for extraction once, centrifuge to discard the residue, and combine twice The filtrate was concentrated under reduced pressure to 1 / 30 of the original volume;
[0025] 3) Add pH 4.5 acetic acid buffer solution and 2.5% phytase concentrate, enzymolyze at 40°C for 3 hours, concentrate under reduced pressure to 1 / 5 volume, remove acetic acid, adjust pH to neutral, and obtain enzymatic hydrolysis solution ;
[0026] 4) The above enzymatic solution was subjected to column chromatography through HZ202 type anion exchange resin, the resin was washed with 500mL deionized water, the eluate was collected, the eluate was concentrated under reduced pressu...
Embodiment 3
[0027] Example 3 Preparation of Carob Extract Containing Inositols
[0028] 1) Weigh 100g of carob, wash, dry, put in a grinder and grind into powder;
[0029] 2) Put the dried carob in a round bottom flask, add 2L of 40% ethanol solution, extract at 80°C for 3 hours, centrifuge, add 1.5L of 40% ethanol solution to the filter residue to extract once, centrifuge to discard the residue, and combine twice The filtrate was concentrated under reduced pressure to 1 / 30 of the original volume;
[0030] 3) Add pH 4.5 acetic acid buffer solution and concentrated solution 4% phytase, enzymolyze at 40°C for 3 hours, concentrate under reduced pressure to 1 / 5 volume, remove acetic acid, adjust pH to neutral, and obtain enzymatic hydrolysis solution ;
[0031] 4) The above enzymatic solution was subjected to column chromatography through HZ202 type anion exchange resin, the resin was washed with 500mL deionized water, the eluate was collected, the eluate was concentrated under reduced pr...
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