Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for regenerating plants by hippeastrum hybridum hort. petal tissue induction

A technique for regenerating plants and petals, applied in the field of plant regeneration induced by Hippeastrum petal tissue, can solve the problem of low reproductive efficiency of Hippeastrum, achieve the effect of improving regeneration efficiency and improving reproductive efficiency

Active Publication Date: 2015-05-06
中山市西江花木研究有限公司
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, Hong Zhizhong (2005) cut the petals of Hippeastrum into 1cm 2 Cultivated in lumps, only a small amount of buds were obtained at the base of the petals, and the reproduction efficiency of Hippeastrum was low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for regenerating plants by hippeastrum hybridum hort. petal tissue induction
  • Method for regenerating plants by hippeastrum hybridum hort. petal tissue induction
  • Method for regenerating plants by hippeastrum hybridum hort. petal tissue induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Effects of TDZ and 2,4-D on the Induction of Petal Projections of Hippeastrum

[0033] To study the effect of different concentrations of TDZ and 2,4-D on the induction rate of Hippeastrum petal protrusions: Take the young inflorescences of Hippeastrum, soak in 70% ethanol for 15 minutes, wash with sterile water for 3 times, cut off the outer bracts, and take out the flower buds , cut the petals perpendicular to the longitudinal axis of the bud, cut them into ring-shaped petal filaments with a width ≤ 1.0mm, and use the petal filaments as explants; place the treated explants horizontally on the induction medium, and place them on the induction medium. 24~26°C, cultured in the dark for 8~12 weeks, the outer surface of the petals produces protrusions; the induction medium is MS medium as the basic medium, and a certain concentration of thiadizuron ( TDZ), 2,4-dichlorophenoxyacetic acid (2,4-D), and 20-40g / L sucrose, pH 5.8-6.0, the experimental results are sho...

Embodiment 2

[0037] Example 2 Effects of 6-BA on Adventitious Buds and Plantlet Regeneration

[0038]To study the effects of different concentrations of 6-BA on the induction of adventitious buds and plant regeneration: take the explants that produced protrusions and transfer them to the regeneration medium, at 24~26°C, 12~14h of light per day, and the light intensity was 1000 Cultivate under the environment of ~1400lux, protrusions form adventitious buds and then form complete plants; the regeneration medium is based on MS medium, and a certain concentration of 6-benzylaminoadenine is added according to Table 2, and 20~40g / L sucrose, pH 5.8~6.0, the experimental results are shown in Table 2. The results showed that the concentration of 6-BA affected the adventitious bud induction rate of Hippeastrum petals and plant regeneration rate. When the concentration of 6-BA was 0-0.5 mg / L, the induction rate of adventitious buds reached 100%, and the plant regeneration rate was ≥92%; when the c...

Embodiment 3

[0043] A method for inducing plant regeneration with Hippeastrum petal tissue, comprising the steps of:

[0044] S1. Explant treatment: take the young inflorescences of Hippeastrum just extracted, wash them with tap water, soak them in 70% ethanol for 15 minutes in an ultra-clean workbench, wash them with sterile water for 3 times, and cut off the outer bracts with a scalpel , and then take out the flower bud with tweezers, cut the petal perpendicular to the longitudinal axis of the flower bud, cut into ring-shaped petal filaments with a width of 0.5-1.0 mm, and use the petal filaments as explants;

[0045] S2. Protrusion induction: quickly transfer the cut ring-shaped petal filament explants, place them horizontally on the induction medium, and culture them under dark conditions at 24-26°C. After about 1 week, the petal fine filaments The silk begins to expand, and at 8 to 12 weeks, a large number of protrusions are produced on the outer surface of the petal and the wound sit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Widthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of plant induction regeneration, and particularly discloses a method for regenerating plants by hippeastrum hybridum hort. petal tissue induction. The method comprises the steps of transversely cutting a hippeastrum hybridum hort. Petal in a manner of being vertical to the vertical axis of a flower bud to obtain ring-shaped petal filaments with the width of less than or equal to 1.0mm, wherein the cut ring-shaped petal filaments are taken as explants; and putting the explants on an induction medium, and culturing at the temperature of 24-26 DEG C under the dark condition until bulged matter is produced on the outer surface of the petal; transferring the explants with the bulged matter onto a regeneration medium, and culturing for 12-14 hours every day at the temperature of 24-26 DEG C in the light environment to enable the bulge matter to be changed into adventitious buds and further complete plants. According to the method, the ring-shaped petal filaments of hippeastrum hybridum hort. are taken as the explants, and a plant regeneration system with an efficient organogenesis way is established; after the method is adopted, the highest bulged matter induction rate reaches 100%, the highest adventitious bud induction rate reaches 100%, and the highest plant regeneration rate reaches 98%; 12.8 small plants can be produced by every explant at most in average, so that the plant regeneration efficiency is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of induced regeneration of plants, and in particular relates to a method for inducing regeneration of plants with petal tissue of Hippeastrum chinensis. Background technique [0002] Hippeastrum ( Hippeastrum hybridum Hort.), also known as Hippeastrum, Amaryllis, Huazhoulan, etc., is an important perennial herb of the genus Hippeastrum in the family Amaryllidaceae. It is native to tropical America and is a world-renowned bulb flower. [0003] The research on tissue culture of Hippeastrum began in the 1970s. At present, most of the bulbs or bulb discs, ovaries or pedicels (including young capsules, young flower stems and young embryos) and hypocotyls are used as starting materials. Obtain callus, adventitious buds or somatic embryos, and then culture to obtain complete plants. When using bulbs or bulb disks as explants, it is necessary to dig out the bulbs, which will cause damage to the bulbs, especially ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 于波朱根发孙映波黄丽丽
Owner 中山市西江花木研究有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com