Preparation method of water-soluble tawny natural pigments in camellia cleifera seed meal

A technology of Camellia oleifera seed meal and natural pigments, which is applied in the direction of natural dyes, sugar derivatives preparation, chemical instruments and methods, etc., can solve the problems of waste, less utilization of camellia oleifera seeds, and waste of residues, etc., and achieve the effect of solving the waste of resources

Inactive Publication Date: 2015-05-06
苏秀珍
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Camellia oleifera seed meal is the residue left after oil-pressing of camellia oleifera seed. Due to the lack of research and understanding of camellia oleifera seed meal in the early stage, these residues after oil-pressing are usually discarded, causing great waste. With the deepening of research People found that in addition to the remaining vegetable oil, these residues also contain a considerable amount of useful nutrients and secondary metabolites such as tea saponin, tea polysaccharides, plant crude protein, and flavonoids.
Therefore, Camellia oleifera seed is currently mainly used to extract camellia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of water-soluble tawny natural pigments in camellia cleifera seed meal
  • Preparation method of water-soluble tawny natural pigments in camellia cleifera seed meal

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] (1) Pulverize 50g of Camellia oleifera seed meal to a particle size of 30 meshes with a pulverizer, put it into a reflux extraction tank, add 125 mL of 60% edible alcohol, heat under reflux for extraction for 2 hours, filter after cooling, and put the filter residue into the extraction tank repeatedly Add 125 mL of 60% edible alcohol to extract for 2 hours, cool and filter, and combine the filtrates. The filtrate is concentrated to dryness under reduced pressure with a rotary evaporator at 45° C. to obtain about 14 g of the extract.

[0027] (2) take 50g amount of polyamide filler (100-200 mesh), pack it in a glass chromatographic column with an inner diameter of 3cm × 60cm long, and equilibrate the chromatographic column with distilled water;

[0028] (3) Take 1 g of the sample obtained in step 1, dissolve it completely with a small amount of distilled water, load it on the polyamide chromatography column packed in step 2, rinse with 0.1% acetic acid...

Example Embodiment

[0031] Example 2

[0032] (1) Pulverize 100g of Camellia oleifera seed meal to a particle size of 20 meshes with a pulverizer, put it into an ultrasonic extractor and add 250mL of 95% edible alcohol, set the temperature to 45°C, and ultrasonically extract it at a frequency of 40KHz for 1 hour, filter, and filter the residue. Repeatedly put into the ultrasonic extractor under the same conditions for repeated extraction twice, filter, combine the filtrate, the filtrate was concentrated to dryness under reduced pressure with a rotary evaporator at 45°C to obtain about 25 g of the extract.

[0033] (2) take 60g amount of polyamide filler (30-60 mesh), pack it in a glass chromatographic column with an inner diameter of 3cm × 60cm long, and equilibrate the chromatographic column with distilled water;

[0034] (3) Take 1 g of the sample obtained in step 1, dissolve it with a small amount of distilled water, and load it on the polyamide chromatography column packed in step 2

[0035] (...

Example Embodiment

[0037] Example 3

[0038] (1) Pulverize 100g of Camellia oleifera seed meal to a particle size of 80 mesh with a pulverizer, put it into an ultrasonic extractor and add 250mL of 95% edible alcohol, set a temperature of 45°C, and ultrasonically extract at a frequency of 40KHz for 1 hour, filter, and filter the residue. Put it into the ultrasonic extractor and repeat the extraction twice under the same conditions, filter, combine the filtrate, and concentrate the filtrate to dryness under reduced pressure with a rotary evaporator at 45°C to obtain about 25 g of the extract.

[0039] (2) Take 55g of polyamide filler (60-90 mesh), pack it in a glass chromatographic column with an inner diameter of 3cm × 60cm long, and equilibrate the chromatographic column with distilled water;

[0040] (3) Take 1 g of the sample obtained in step 1, dissolve it with a small amount of distilled water, and load it on the polyamide chromatography column packed in step 2.

[0041] (4) Elution is carr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of water-soluble tawny natural pigments in camellia cleifera seed meal. By taking residues after squeezing tea oil from camellia cleifera seed as a raw material, the method for preparing the high purity tawny pigments by the following processes: preparing raw materials of camellia cleifera seed meal; crushing by a crusher; extracting by edible alcohol; filtering; concentrating an extracting solution; carrying out polyamide column chromatography; carrying out stepwise elution on a mixture containing 0.1% acetate solution and an edible alcohol solution; and obtaining a tawny pigment product. The product comprises various flavone glycoside compounds, the main component comprises two compounds, and the purity of flavonoid pigment components in the final product detected by HPLC can reach over 70-95%. The obtained tawny pigments can be added into foods or cosmetics, particularly transparent liquid shampoo or shower gels which are clear and natural in color and have sense of beauty and natural antioxidant activity.

Description

technical field [0001] The invention relates to the extraction and preparation technology of a water-soluble brown natural pigment extracted from camellia oleifera seed dregs, and belongs to the technical field of food and medicine Background technique [0002] Camellia oleifera seed is the mature seed of Camellia oleifera Abel or C. meiocarpa Hu, ms., which is a unique edible vegetable oil resource in China. Camellia oleifera seed meal is the residue left after oil-pressing of camellia oleifera seed. Due to the lack of research and understanding of camellia oleifera seed meal in the early stage, these residues after oil-pressing are usually discarded, causing great waste. With the deepening of research It is found that in addition to the remaining vegetable oil, these residues also contain a considerable amount of useful nutrients and secondary metabolites such as tea saponin, tea polysaccharides, plant crude protein, and flavonoids. Therefore, Camellia oleifera seed is cu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09B61/00C09B67/54C07H17/07C07H1/08
CPCC07H1/08C07H17/07C09B61/00C09B67/0096
Inventor 陈全成石欣蕾张伟云苏秀珍洪珠凤
Owner 苏秀珍
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap