A special primer, method and kit for identifying Meishan pig or its meat products

A technology for meat products and kits, which is applied in the fields of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of simple operation, beneficial to practical application, and solving the problem of counterfeiting

Inactive Publication Date: 2016-08-24
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no method of identifying Meishan pig and its meat products by molecular identification methods at home and abroad. A method that can distinguish Meishan pig products from other varieties of pork products has been established to resolve counterfeit sales disputes involving Meishan pigs and their meat products. In order to effectively protect the market position and brand image of local Meishan pig breeds

Method used

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  • A special primer, method and kit for identifying Meishan pig or its meat products
  • A special primer, method and kit for identifying Meishan pig or its meat products
  • A special primer, method and kit for identifying Meishan pig or its meat products

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Selection of the optimal annealing temperature during amplification of Meishan 1

[0036] 1. Take 100 mg of Meishan sow fresh meat, put it into a grinder, add 1000 μL of distilled water, grind for 3 minutes, take 100 μL of grinding liquid, add 1 mL of DNA extraction buffer, the extraction buffer is 10mmol / LTris-HCl, 0.1mol / L EDTA , 0.5% SDS, then add 25 μL proteinase K to make the final concentration reach 100 μg / mL, mix well, and water bath at 50°C for 3 hours. Extract once with an equal volume of phenol, collect the aqueous phase by centrifugation at 2500 r / min, and extract once with an equal volume of a mixture of phenol, chloroform and isoamyl alcohol, the volume ratio of the phenol, chloroform and isoamyl alcohol being 25:24 : 1,2500r / min centrifugal collection water phase; Extract once with the mixture of equal volume chloroform and isoamyl alcohol, the volume ratio of described chloroform and isoamyl alcohol is 24:1; Add the LiCl of equal volume 5mol / L ...

Embodiment 2

[0040] Example 2 Identification of the fresh meat of Meishan pig, Mei Du binary hybrid and Du Chang San Yuan hybrid

[0041] 1. Take 100mg of the raw meat of Meishan pig sows, Meidu binary hybrid sows, Meishan pig boars and Meidu binary hybrid boars and the frozen meat of Du Dachang Sanyuan hybrid, put them into a grinder and add 1000 μL Distilled water, grind for 5 min, take 100 μL grinding liquid, add 1 mL DNA extraction buffer, the extraction buffer is 10 mmol / L Tris-HCl, 0.1 mol / L EDTA, 0.5% SDS, and then add 25 μL proteinase K to make the final concentration reach 100 μg / mL, mix well, and water bath at 50°C for 3 hours. Extract once with an equal volume of phenol, collect the aqueous phase by centrifugation at 2500 r / min, and extract once with an equal volume of a mixture of phenol, chloroform and isoamyl alcohol, the volume ratio of the phenol, chloroform and isoamyl alcohol being 25:24 : 1,2500r / min centrifugal collection water phase; Extract once with the mixture of e...

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PUM

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Abstract

The invention discloses a special primer, a method and a kit for identifying a Meishan pig or a meat product thereof. A sequence of the special primer is as shown in SEQ ID NO.1. The method comprises the following steps: (1), extracting genomic DNA (deoxyribonucleic acid) of fresh meat and / or the meat product; (2), taking genomic DNA in the step (1) as a template, and performing a PCR (polymerase chain reaction) by utilizing the primer with the nucleotide sequence as shown in SEQ ID NO.1. The method further comprises the following steps: (3), creating an electrophoretogram of amplified fragments in the PCR in the step (2); or (4), according to the electrophoretogram in the step (3), identifying the fresh meat and / or the meat product of the Meishan pig. The special primer and the method are utilized to effectively solve an identification problem of a Meishan pig variety and can distinguish the Meishan pig variety from other pig meat varieties or beef and mutton. The identification method is simple to carry out and accurate in result.

Description

technical field [0001] The invention relates to the field of biology, in particular to a special primer, a method and a kit for identifying Meishan pigs or their meat products. Background technique [0002] Meishan pigs are native to Shanghai Jiading, Baoshan, Songjiang and other districts and counties in the Taihu Lake Basin and Kunshan, Taicang and other cities and counties in Jiangsu. characteristic. The Meishan pig breed in Jiading District has fresh and tender muscles, fine and dense muscle fibers, less water content, rich muscle fat, marble-shaped, commonly known as "pork belly", with a mellow flavor, delicate and soft. With the improvement of people's living standards, the quality requirements of livestock and poultry products are getting higher and higher, and many varieties such as Waisanyuan, which are cultivated for the purpose of pursuing high yield, are becoming less and less popular in China. , refined, high-quality meat. Meishan pork is favored by Shanghai ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 史子学马志永张国平朱建忠王勃陈建生陆林根卫金良唐赛涌费琼慧于庆华魏建超刘珂李蓓蓓邵东华
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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