Full-automatic thermal cycler hot cover

A thermal cycler, fully automatic technology, used in medical testing, biological fields, can solve problems such as burns, difficult integration, etc., to achieve the effects of easy assembly, uniform force, and simple structure

Active Publication Date: 2015-05-20
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The heating lid of the traditional thermal cycler is mostly controlled manually, such as the ABI-Veriti gradient PCR instrument, the user opens the heating lid during the experiment, and occasionally accidentally touches the heating surface of the heating lid and bur

Method used

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  • Full-automatic thermal cycler hot cover
  • Full-automatic thermal cycler hot cover
  • Full-automatic thermal cycler hot cover

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0024] The following examples and attachments are combined Figure 1-7 The present invention is explained further. The following examples are only used to illustrate the present invention, but not to limit the scope of implementation of the present invention.

[0025] This embodiment case combines the entire PCR thermal cycler, and illustrates through a PCR amplification experiment operation. At the beginning of the experiment, the thermal cycler is powered on, the touch screen 301 is initialized, and the hot cover open command button on the touch screen is clicked. If the hot cover 200 of the automatic thermal cycler is originally opened, the drive motor 204 will not work and the user will install Place the 96-well plate or separate PCR tube of the sample in the heat trap. Click the hot lid close command button to drive the motor 204 to rotate and drive the threaded screw 203 to rotate. The threaded screw 203 is connected to the propelling plate 202, and the rotation of the t...

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PUM

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Abstract

The invention relates to a full-automatic thermal cycler hot cover. When a propulsion plate moves, a guide plate and a hot cover supporting plate drive a heating cover plate to synchronously move along a groove and a boss in a side plate of a thermal cycler; when the guide plate moves to slope sections of the groove and the boss, a limiting sensor detects the limiting position of the propulsion plate and sends a signal, a control device receives the signal and stops the rotation of a motor, and the propulsion plate, the guide plate and the hot cover supporting plate stop moving; the groove and the boss in the slope sections ensure that a spring can be pressed downwards by the guide plate, and the spring can produce downward pressure to the hot cover supporting plate to ensure that the heating cover plate which is fixed with the hot cover supporting plate is pressed downwards; and when resistance is applied to the heating cover plate, a counterforce can be produced to the spring, and in the dynamic process, the heating cover plate can adapt to optimal downward pressure by virtue of the automatic elasticity regulation of the spring so as to ensure that the heating cover plate has moderate pressure on 96-pore plates or PCR pipes.

Description

technical field [0001] The invention relates to a heat cover of a fully automatic thermal cycler, which is suitable for an automatic biochemical detection system and belongs to the fields of biology, medical detection and the like. Background technique [0002] Before the advent of thermal cyclers, polymerase chain reaction (PCR) was a laborious process. With the development of Peltier modules, special consumables and enzyme technology (such as hot start function), the PCR technology and thermal cycler have been greatly improved and developed, making PCR reactions easier to perform. In the current thermal cycler structure, the thermal cover is an extremely important part. The flat top cover in contact with the sample container will be heated by the heated cover to at least 5°C above the maximum reaction temperature to ensure that the sample will not condense on the inner surface of the sample container cover. And when the thermal cycler is in use, it is necessary to adjust...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/00
CPCB01L7/52
Inventor 何农跃邬燕琪陈慧陈柱
Owner SOUTHEAST UNIV
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