Full-automatic thermal cycler hot cover

[0024] The following examples and attachments are combined Figure 1-7 The present invention is explained further. The following examples are only used to illustrate the present invention, but not to limit the scope of implementation of the present invention.

[0025] This embodiment case combines the entire PCR thermal cycler, and illustrates through a PCR amplification experiment operation. At the beginning of the experiment, the thermal cycler is powered on, the touch screen 301 is initialized, and the hot cover open command button on the touch screen is clicked. If the hot cover 200 of the automatic thermal cycler is originally opened, the drive motor 204 will not work and the user will install Place the 96-well plate or separate PCR tube of the sample in the heat trap. Click the hot lid close command button to drive the motor 204 to rotate and drive the threaded screw 203 to rotate. The threaded screw 203 is connected to the propelling plate 202, and the rotation of the threaded screw 203 causes the propelling plate to slide forward on the sliding guide 207 through the sliding guide groove 208 connected to it. Since the push plate 202 is connected to the heat cover support plate 214 and the heating cover plate 212 through the support column 209, and is connected to the guide plate 201 through the connecting piece 218, when the push plate 202 moves, the guide plate 201 is simultaneously driven along the thermal cycler The groove 104 and the boss 103 on the side plates 101 move. When moving to the side plate groove 104 and the boss 103 slope section 106, 108, the protruding sheet (not shown) on the push plate 202 is detected by the thermal lid closing limit sensor 211, and a signal is sent, and the control device receives The signal controls the drive motor 204 to stop rotating. When the guide plate 201 slides down the slope sections 106, 108, it will compress the spring 215 on the support column 209. The spring 215 will generate downward pressure to press the heating cover support plate 214 and the heating cover plate 212, so that the heating cover 212 will Press down on the 96-well plate or PCR tube. When the heating cover plate 212 is pressed down to a certain degree, it will receive resistance. When the resistance is too large, the heating cover plate 212 and the heating cover support 214 plate will generate upward rebound force. In this dynamic process, the heating cover can adapt to the most Good downforce. The positions of the four support posts 209 and the elastic force of the spring 215 can ensure that the force of each part of the heating cover 212 is uniform. A limit post is set at the bottom of the push plate to limit the maximum pressing and rising position of the heat cover. The push plate 202 is connected with the three through holes 217 on the guide plate 201 through the connecting piece 218 by screws. The screws can move upward along the through holes 217 and cannot move laterally, so the push plate 202 can maintain the horizontal direction without being deformed and pressed down. During the entire process of closing the heating cover 212, the pushing plate 202 mainly controls the horizontal movement of the heating cover 212, and the guide plate 201, the side plate groove 104 and the boss 103 mainly control the up and down movement of the heating cover 212. After the thermal cover 200 of the entire thermal cycler is closed, the user can set experimental parameters on the touch screen, such as amplification cycle temperature, temperature holding time, cycle number, etc. After setting, click the run button to start the experiment. After the experiment is over, the user can click the command button to open the hot lid. The opening action of the thermal cycler hot lid 200 is reverse to the closing action. When the protruding sheet (not shown) on the push plate 202 is opened by the hot lid limit sensor When 210 is detected, the driving motor 204 stops and the thermal cover 200 of the thermal cycler is fully opened. Users can take out the amplified samples for downstream detection or hybridization experiments.

[0026] During the whole experiment process, the action of the hot cover is completely completed by clicking the button on the touch screen. The experiment is safe and convenient, and the experiment effect is excellent.