Sea cucumber gender rapid non-destructive identification method

An identification method and gender technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as inability to achieve non-damage discrimination, increase production costs during the temporary breeding period, and difficulty in determining the sex of sea cucumbers, so as to eliminate human disturbance and the risk of miscarriage after exposure, avoiding the loss of relatives, and the effect of good operability

Inactive Publication Date: 2015-05-27
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
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AI Technical Summary

Problems solved by technology

However, the sex identification of sea cucumbers has always been a difficult point in the cultivation of sea cucumber seedlings. In the past, it could only be identified through dissection or ovulation, and it was impossible to achieve non-invasive discrimination.
Ideally, the success of the breeding can be guaranteed when the male to female ratio of sea cucumbers reaches about 10-20:1. However, since it is difficult to determine the sex of sea cucumbers, a large number of individuals must be preserved in the process of temporary breeding and maturation of sea cucumbers to ensure male and female sex. Ratio, greatly increasing the production cost during the holding period
At the same time, the degree of gonad development of sea cucumbers can only be identified through anatomical methods, which requires the loss of species, which will cause large economic losses

Method used

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Examples

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Embodiment

[0015] Example: Gender identification in the process of promoting ripening of ginseng

[0016] In May 2013, a quick identification of the sex of the relatives was carried out in a japonicus nursery in Rushan, Shandong. The scanning equipment was a portable veterinary ultrasonic scanner equipped with a 14Mhz fully waterproof linear array probe. The gonads of 30 japonicus individuals with a total wet weight ranging from 150g to 300g were scanned in the air. The scanning position was the dorsal side of the head. The sex was determined according to the echo image, followed by anatomical verification. The identification accuracy rate was as high as 97%.

[0017] In March 2014, a rapid identification of the sex of relatives was carried out in a japonicus nursery in Rushan, Shandong. The scanning equipment was a veterinary portable ultrasonic scanner equipped with a 14Mhz fully waterproof linear array probe. Scan the gonads of 12 japonicus japonicus individuals with a total wet weigh...

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Abstract

The invention relates to the technical field of aquaculture, in particular to a sea cucumber gender rapid non-destructive identification method. The method includes the steps of directly scanning sea cucumber with developed gonads for examining with a medical ultrasound scanner and determining the gender of the sea cucumber according to typical echo characteristics of male and female gonad tubules of the sea cucumber on the basis of an obtained echo image, wherein the acoustic image characteristics of female gonads are that a single gonad tubule ultrasound image is uniform in brightness, the tubule wall is not different from the gonad cavity in the echo intensity, and the overall ultrasound image brightness is significantly higher than a digestive tract tissue, and the acoustic image characteristics of male gonads are that the echo intensity of the tubule wall of a single gonad tubule is significantly higher than the gonad cavity, and the overall ultrasound image brightness is in the same with or lower than the digestive tract tissue. By means of the sea cucumber gender rapid non-destructive identification method, the sea cucumber gender can be identified without anatomizing and injuring the sea cucumber and is high in identification accuracy. The sea cucumber gender rapid non-destructive identification method can be widely used in male and female sea cucumber screening during larval sea cucumber cultivation, gonad development process monitoring and maturity assessment.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a fast and non-destructive identification method for the sex of sea cucumbers. Background technique [0002] Sea cucumber is one of the important marine species in my country. It is often necessary to identify the male and female sex of sea cucumbers in the process of multiplication, breeding and scientific experiments of sea cucumbers. However, the sex identification of sea cucumber has always been a difficult point in the cultivation of sea cucumber seedlings. In the past, it could only be identified by dissection or after ovulation and semen ovulation, and it was impossible to achieve non-injury discrimination. Ideally, the success of the breeding can be guaranteed when the male to female ratio of sea cucumbers reaches about 10-20:1. However, since it is difficult to determine the sex of sea cucumbers, a large number of individuals must be preserved in the process of temp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 许强刘石林张立斌杨红生张涛
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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