Method for separating and purifying cordyceps polysaccharide contained in cordyceps militaris solid culture medium
A technology of solid culture medium and Cordyceps polysaccharide, which is applied in the field of traditional Chinese medicine, can solve the problems that the utilization of solid culture residues is rarely reported, and achieve the effect of easy operation and high extraction rate
Inactive Publication Date: 2015-05-27
HAIAN HONGSHOU BIOTECH
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Problems solved by technology
[0002] Cordyceps militaris is a precious medicinal fungus and high-grade tonic. It uses rice and silkworm chrysalis powder as the main raw materials to make a solid medium to artificially cultivate the fruiting body of Cordyceps militaris. However, after the fruiting body is harvested, the solid culture medium covered with mycelium The use of residues is rarely reported
Method used
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Experimental program
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Effect test
Embodiment 1
[0013] The separation and purification method of Cordyceps militaris polysaccharide in the Cordyceps militaris solid culture medium of the present embodiment comprises the following steps:
[0014] 1) Collect the solid culture residue of Cordyceps militaris, crush through an 80-mesh sieve, and degrease;
[0015] 2) Extract the product obtained in step 1) in a water bath, remove impurities, and concentrate;
[0016] 3) Separate the product obtained in step 2) through an ion exchange column, and elute with ethanol to obtain an eluent. The separation conditions of the ion exchange column are as follows: the ratio of material to water is 1:16, the pH of the ion column is 8; the elution flow rate is 1 drop / 2 seconds.
[0017] 4) The eluate collected in step 3) is subjected to multiple ultrafiltration, and finally concentrated to obtain the polysaccharide of Cordyceps militaris in the solid medium of Cordyceps militaris.
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The invention provides a method for separating and purifying cordyceps polysaccharide contained in a cordyceps militaris solid culture medium. The method comprises the following steps of: (1) collecting residual cordyceps militaris solid culture mediums, crushing, screening through a screen with 80 meshes, and degreasing; (2) carrying out water bath extraction on a product obtained from the step (1), then removing impurities, and concentrating; (3) separating the product obtained from the step (2) through an ion exchange column, and eluting by using ethanol to obtain an eluent; (4) carrying out ultrafiltration for multiple times on the eluent collected from the step (3), and finally concentrating to obtain the cordyceps polysaccharide contained in the cordyceps militaris solid culture medium. The method for separating and purifying the cordyceps polysaccharide contained in the cordyceps militaris solid culture medium, which is provided by the invention, is easy to operate and high in extraction rate.
Description
technical field [0001] The invention relates to a method for separating and purifying polysaccharides from Cordyceps militaris in a solid medium of Cordyceps militaris, belonging to the technical field of traditional Chinese medicines. Background technique [0002] Cordyceps militaris is a precious medicinal fungus and high-grade tonic. It uses rice and silkworm chrysalis powder as the main raw materials to make a solid medium, and artificially cultivates the fruiting bodies of Cordyceps militaris. The utilization of residues is rarely reported. The content of cordycepin in the solid culture of Cordyceps militaris is about 5‰, the total sugar content is 21.93%, the protein is still about 10% (2.1% higher than before cultivation), the content of Cordyceps polysaccharide is 12.86%, in addition, it also contains alkaloids, Terpenoids, sterols, glycosides, phenols, enzymes, vitamins, Mn-SOD and other active ingredients. Contents of the invention [0003] The purpose of t...
Claims
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Inventor 周余庆
Owner HAIAN HONGSHOU BIOTECH