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A kind of polypeptide bacteria, its preparation method and application

A technology for synthesis of peptide bacteria and solid-phase peptides, which is applied in the preparation methods, applications, chemical instruments and methods of peptides, etc., can solve the problems of decreased immunity of livestock and poultry, adverse effects of vaccination, loss of disease resistance, etc. Achieve the effect of enhancing humoral immunity, enhancing humoral immune function, and reducing the incidence of disease

Active Publication Date: 2017-06-13
SHANDONG SCIHEALTH BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 2. Decrease the immune system of livestock and poultry
Antibiotics can also lead to the reduction of antigen quality, which directly affects the immune process and thus adversely affects the vaccination
[0008] 3. Cause endogenous infection and double infection of livestock and poultry
[0010] 4. Cause residues in livestock products and the environment
Some stable antibiotics can remain stable for a long time after being excreted into the environment, resulting in drug residues in the environment
These remaining drugs slowly accumulate in the human body and other plants through animal products and the environment, and finally gather in the human body in various ways, causing the human body to produce a large number of drug-resistant strains, losing resistance to certain diseases, or due to a large number of Accumulate and produce toxic effects on the body

Method used

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  • A kind of polypeptide bacteria, its preparation method and application
  • A kind of polypeptide bacteria, its preparation method and application
  • A kind of polypeptide bacteria, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] This example is an example illustrating the preparation method of the polypeptide bacteriocin.

[0085] Establish the synthesis and purification production process of polypeptide bacteria suitable for large-scale production, and provide guarantee for the research and application of polypeptide bacteria products. Using the principle of Fmoc-solid-phase polypeptide synthesis, 2-Chlorotrityl Chloride resin is used as a solid-phase carrier, HOBt / TBTU / DIEA / is used as a catalyst, DMF is used as a solvent and cleaning agent, and TFA / EDT / H 2 O is the cutting agent, using the self-designed and developed synthesizer as the equipment, to establish the compound antimicrobial peptide synthesis production process and equipment system suitable for large-scale production; use water-acetonitrile as the binary gradient elution mobile phase, and use 1% trifluoro Acetic acid is an ion pair, and the product is purified by RP-HPLC. The purified product is a white powder after freeze-drying,...

Embodiment 2

[0199] This embodiment tests the effect of the polypeptide bacteria obtained in Example 1 on Holstein cows.

[0200] 1. Test method:

[0201] From September 30, 2013 to November 10, 2013, a total of 40 days, of which the first 10 days are the pre-test period, and the last 30 days are the formal test period.

[0202] 1. Test group description:

[0203] Select 40 healthy lactating cows with similar parity (1-2 parity), lactation days (80-120 days), and milk production, and randomly divide them into two groups to determine the test group and the control group.

[0204] group group Quantity (head) Group A test group 20 Group B control group 20

[0205] 2. After the pre-test period, analyze the index data such as milk production during the period. If there is no significant difference between the experimental group and the control group, enter the formal experimental period.

[0206] 3. Feeding method of the experimental group

[0207] (1) Rout...

Embodiment 3

[0232] This embodiment tests the effect of the polypeptide bacteriocin obtained in Example 1 on the immune function and growth performance of SPF chickens.

[0233] (1) Test materials

[0234] 1 Test drugs and reagents

[0235] 1.1 Product sample: the polypeptide bacteria obtained in Example 1, the purity is greater than 98%.

[0236] 1.2 Reagents for rosette test

[0237] Anticoagulant (3.8% sodium citrate solution or 200U / mL heparin saline solution), Hank's solution (no Ca2+, Mg2+, pH7.2-7.4), lymphocyte separation medium, 1% sheep red blood cell (SRBC) suspension solution, inactivated calf serum, fixative (0.8% glutaraldehyde), Wright's stain, Giemsa stain, all prepared in the laboratory.

[0238] 1.3 Test kits

[0239]Serum total protein (TP) detection kit was purchased from Nanjing Jiancheng Biotechnology Research Institute, batch number 20110509, and albumin (ALB) detection kit was purchased from Nanjing Jiancheng Biotechnology Research Institute, batch number 201105...

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Abstract

The present invention relates to a polypeptide bacterin, its preparation method and application. The polypeptide bacteriocin provided by the invention has the functions of improving immunity, repairing cell tissue and / or antibacterial and growth promoting. The chemical synthesis method provided by the invention is simple and easy, and can meet the needs of the livestock and poultry industry and the market. At the same time, it meets the needs of large-scale industrial production.

Description

technical field [0001] The present invention relates to a polypeptide bacterin, its preparation method and application. Specifically, the present invention provides a polypeptide bacterin with the effects of improving immunity, repairing cell tissue, and / or antibacterial and growth-promoting, its preparation method and its application in the preparation of antibiotic substitutes or in the livestock and poultry industry. Background technique [0002] Since Moore et al. (1946) reported for the first time that adding antibiotics to feed can significantly increase the daily weight gain of broilers, people have been researching and applying antibiotics more and more widely. More than 60 kinds of antibiotics have been used in animal husbandry successively. It has played an important role in preventing and controlling, improving feed utilization, and promoting the growth of livestock and poultry. However, with the extensive use of antibiotics, especially unscientific abuse, proble...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/06C07K1/04C07K1/20A61K38/08A61P37/04A61P31/04A61P31/14A23K20/147A23K20/195
Inventor 吕志成杨赛鲍隆
Owner SHANDONG SCIHEALTH BIOLOGICAL TECH CO LTD
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