PCr-NHEJ (non-homologous end joining) carrier as well as construction method of pCr-NHEJ carrier and application of pCr-NHEJ carrier in site-specific knockout of bacterial genes
A construction method and a technology for targeted knockout, applied in the field of genetic engineering, can solve the problems of cumbersome operation, difficult operation, and insufficient gene knockout efficiency, and achieve the effects of low operation difficulty, reduced operation difficulty, and high gene knockout efficiency.
Inactive Publication Date: 2015-06-03
GUANGDONG MEDICAL UNIV
4 Cites 54 Cited by
AI-Extracted Technical Summary
Problems solved by technology
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreAbstract
The invention belongs to the technical field of gene engineering, in particular to a pCr-NHEJ (non-homologous end joining) carrier as well as a construction method of the pCr-NHEJ carrier and an application of the pCr-NHEJ carrier in site-specific knockout of bacterial genes. The sequence of the pCr-NHEJ carrier is shown as the SEQ ID NO.1. The application of the pCr-NHEJ carrier in site-specific knockout of the bacterial genes is provided. The technical principle is as follows: NHEJ and CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR associated protein 9) are jointly applied, so that after DNA (deoxyribonucleic acid) is broken when a CRISPR-Cas9 system cuts bacterial genomic DNA, the NHEJ system can be connected with a broken DNA end automatically, bacteria are survived, double-strand DNA having homology with a target sequence is not required to be introduced artificially to repair the broken DNA end, and the operation steps of the CRISPR-Cas9 technique are simplified.
Application Domain
Technology Topic
CRISPR-Associated ProteinsUnclassified Bacteria +9
Image
Examples
- Experimental program(5)
- Effect test(1)
Example Embodiment
Example Embodiment
Example Embodiment
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Similar technology patents
gRNA subjected to wild type T cell TCR alpha chain knockout and method
InactiveCN107236741AHigh gene knockout efficiencyEasy to prepareImmunoglobulin superfamilyStable introduction of DNAMolecular biologyT cell immunity
Owner:THE FIFTH AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
Method and system for managing caller in household network, household gateway
ActiveCN101299694AEasy to operateConsider flexibilityUser identity/authority verificationData switching by path configurationAuthorizationAccess network
Owner:GLOBAL INNOVATION AGGREGATORS LLC
Method for operating user interface and mobile terminal
ActiveCN101833419AEasy to operateRich languageSubstation equipmentInput/output processes for data processingDuration timeUser interface
Owner:HUAWEI DEVICE CO LTD
Composite material of poly lactic acid / natural faric, and production method
Owner:TORAY FIBER RES INST(CHINA) CO LTD
Parking space inquiry booking system and inquiry booking method thereof
InactiveCN102831785AHigh degree of intelligenceEasy to operateIndication of parksing free spacesParking spaceTraffic crash
Owner:CHENGDU YUFAN TECH CO LTD
Classification and recommendation of technical efficacy words
- High gene knockout efficiency
- Easy to operate
gRNA subjected to wild type T cell TCR alpha chain knockout and method
InactiveCN107236741AHigh gene knockout efficiencyEasy to prepareImmunoglobulin superfamilyStable introduction of DNAMolecular biologyT cell immunity
Owner:THE FIFTH AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV