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A system for high-throughput testing str typing and its detection method

A high-throughput and systematic technology, applied in the biological field, can solve the problems of limiting the number of detectable loci, unable to measure STR sequence polymorphism, unable to quickly detect DNA information, etc.

Active Publication Date: 2017-12-26
SHANGHAI CRIMINAL SCI TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional STR genotype detection method uses capillary electrophoresis to perform fluorescence detection on the length of STR fragments, and returns the fluorescence peak position corresponding to the core repeat number. The method can only obtain STR length polymorphism information, and cannot simultaneously determine the sequence polymorphism of STR
At the same time, the use of fluorescence detection requires fluorescent labeling of the primers used for amplification, which increases the error of fluorescence detection and limits the number of detectable loci for STR typing

Method used

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  • A system for high-throughput testing str typing and its detection method
  • A system for high-throughput testing str typing and its detection method
  • A system for high-throughput testing str typing and its detection method

Examples

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Embodiment 1

[0031]A system for high-throughput testing of STR typing includes a biological mixture system comprising at least one sample solution and at least one different primer pair for an STR locus, wherein the primer pair can be used to amplify Increase at least one of the STR loci, the STR is selected from autosomes and / or Y chromosomes; a multiplex amplification system, the multiplex amplification system is used for multiplex amplification of the STR by the at least one different primer pair Gene loci, forming at least one STR locus amplification product; and a detection system, wherein the detection system is a high-throughput sequencing device for detecting at least one type of the STR loci. Therefore, the autosome and / or Y chromosome STR compound amplification typing detection can be realized through high-throughput sequencing technology, and the detection accuracy and the recognition degree of individual identification can be improved.

[0032] Wherein, when the STR is selected...

Embodiment 2

[0117] A system for high-throughput testing of STR typing includes a biological mixture system comprising at least one sample solution and at least one different primer pair for an STR locus, wherein the primer pair can be used to amplify Increase at least one of the STR loci, the STR is selected from autosomes and / or Y chromosomes; a multiplex amplification system, the multiplex amplification system is used for multiplex amplification of the STR by the at least one different primer pair Gene loci, forming at least one STR locus amplification product; and a detection system, wherein the detection system is a high-throughput sequencing device for detecting at least one type of the STR loci. Therefore, the rapid detection of STR typing on autosomes and / or Y chromosomes can be realized by high-throughput sequencing technology, and the detection accuracy and the recognition degree of individual identification can be improved.

[0118] Wherein, when the STR is selected from the Y c...

Embodiment 3

[0177] A system for high-throughput testing of STR typing includes a biological mixture system comprising at least one sample solution and at least one different primer pair for an STR locus, wherein the primer pair can be used to amplify Increase at least one of the STR loci, the STR is selected from autosomes and / or Y chromosomes; a multiplex amplification system, the multiplex amplification system is used for multiplex amplification of the STR by the at least one different primer pair Gene loci, forming at least one STR locus amplification product; and a detection system, wherein the detection system is a high-throughput sequencing device for detecting at least one type of the STR loci. Therefore, the autosome and / or Y chromosome STR compound amplification typing detection can be realized through high-throughput sequencing technology, and the detection accuracy and the recognition degree of individual identification can be improved.

[0178] Wherein, when the STR is selecte...

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Abstract

A high-throughput testing STR typing system and detection method thereof of the present invention, the method comprising the steps of (S100) extracting sample genomic DNA; (S200) preparing a high-throughput sequencing-based STR compound amplification biological mixing system, Using the STR locus for multiplex amplification of the sample genomic DNA; and (S300) high-throughput sequencing technology to detect the genotype of the STR locus for identification and identification of individuals, applicable to the field of forensic science STR typing test.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection system and method based on high-throughput sequencing of short tandem repeats (short tandem repeats, STR) genotype. Background technique [0002] In recent years, the technical means of identifying biological information through biological detection technology has become more and more common. The basic task of DNA testing in forensic science is to solve the problems of individual identification and paternity identification in judicial practice. No matter in criminal cases, civil cases, major accidents or disasters, DNA typing plays a vital role in clarifying the nature of the case and identifying suspects, parties or victims. The short tandem repeat (STR) typing test technology based on capillary electrophoresis has been widely used in forensic science DNA typing laboratories due to its high degree of automation, rapidity, sensitivity, accuracy, stability, and good repeat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 赵雪莹马克李辉曹禺平原刘文斌
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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