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Simple and convenient detection method for phosphorus content in meningitis vaccine

A meningitis vaccine and detection method technology, which is applied in the field of simple detection of phosphorus content in meningitis vaccines, can solve the problems of low efficiency, complex process, and difficult operation, and achieve the effects of cost reduction, accurate measurement, and simple process

Active Publication Date: 2015-06-03
ZHEJIANG VACIN BIO PHARMA LTD
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  • Abstract
  • Description
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Problems solved by technology

[0002] Group A meningococcal polysaccharide vaccine is referred to as meningitis vaccine, the third 2010 edition of "Chinese Pharmacopoeia" requires the determination of phosphorus content in the meningitis vaccine, "Chinese Pharmacopoeia" third 2010 edition, appendix VIIA phosphorus determination method Only qualitative requirements have been given in the paper, and no specific and unified method for measuring phosphorus content has been given; each vaccine manufacturer in the prior art has different methods for the meningitis vaccine phosphorus content measurement, complicated process, difficult operation, long process, and high efficiency. low cost and high cost, therefore, the prior art has the problems and deficiencies of difficult operation, low efficiency and high cost

Method used

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  • Simple and convenient detection method for phosphorus content in meningitis vaccine
  • Simple and convenient detection method for phosphorus content in meningitis vaccine
  • Simple and convenient detection method for phosphorus content in meningitis vaccine

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Embodiment

[0037] Determination content: Determination of phosphorus content of group A meningococcal polysaccharide vaccine

[0038] Reagent preparation:

[0039] Test sample, group A meningococcal polysaccharide vaccine;

[0040] Sulfuric acid, analytically pure;

[0041] Perchloric acid, analytically pure;

[0042] Water for Injection;

[0043] Ammonium molybdate solution, 0.04mol / L;

[0044] reducing agent;

[0045] determination

[0046] Ambient air pressure: one atmosphere; Ambient temperature: room temperature;

[0047] Facilities and instruments: sterile room; test tube; digestion furnace DK 20S; vortex mixer; ultraviolet-visible spectrophotometer UV1700;

[0048] The operation process includes the preparation process of reducing agent and the determination process of phosphorus content;

[0049] The preparation process of the reducing agent is as follows: sodium bisulfite, analytically pure, 6g; sodium sulfite, analytically pure, 1.2g; 1-amino-2-naphthol-4-sulfonic acid,...

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Abstract

The invention discloses a simple and convenient detection method for phosphorus content in a meningitis vaccine. The detection method comprises reagent preparation and an operation process. By virtue of a technical scheme which can precisely determine phosphorus content in a meningitis vaccine sample through ways of carbonizing, secondary digestion, reducing and ultraviolet absorption determination, the method disclosed by the invention can achieve the purposes of simplifying operation, improving efficiency and reducing cost in the detection of the phosphorus content in the meningitis vaccine.

Description

technical field [0001] The invention relates to a method for detecting the phosphorus content of vaccines, in particular to a simple and convenient method for detecting the phosphorus content of meningitis vaccines used for detecting the phosphorus content in group A meningococcal polysaccharide vaccines. Background technique [0002] Group A meningococcal polysaccharide vaccine is referred to as meningitis vaccine, the third 2010 edition of "Chinese Pharmacopoeia" requires the determination of phosphorus content in the meningitis vaccine, "Chinese Pharmacopoeia" third 2010 edition, appendix VIIA phosphorus determination method Only qualitative requirements have been given in the paper, and no specific and unified method for measuring phosphorus content has been given; each vaccine manufacturer in the prior art has different methods for the meningitis vaccine phosphorus content measurement, complicated process, difficult operation, long process, and high efficiency. Low, hig...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/44
Inventor 杨乾敏杨云霞胡文龙谢丹霞
Owner ZHEJIANG VACIN BIO PHARMA LTD