Microscopic analyzing device

Abstract

The invention provides a microscopic analyzing device. The microscopic analyzing device comprises a laser source device, a light transmission device, a stochastic optical reconstruction microscopic device, an atomic force microscopic device, a sample bearing device and an information integrating and outputting device. A light source beam provided by the laser source device is guided into the stochastic optical reconstruction microscopic device through the light transmission device, so that a sample to be detected on the sample bearing device is subjected to optical reconstruction microscopic imaging; simultaneously, the atomic force microscopic device carries out atomic force microscopic imaging at the same position; the information integrating and outputting device integrates and outputs stochastic optical reconstruction microscopic information and atomic force microscopic information; therefore, the problem that the sample cannot be qualitatively analyzed by the existing atomic force microscopic imaging can be solved; and thus, the microscopic analyzing device can be widely applied in the fields of biology, polymers and materials.

Description

technical field [0001] The invention relates to a combination technology of a stochastic optical reconstruction micro-device and an atomic force micro-device, belonging to the field of microanalysis. Background technique [0002] Optical microscope is an essential tool for biologists to do research and explore the mysteries of life. Light microscopy has been used to gain a first glimpse at the intricate structure and morphology inside cells. However, limited by the optical diffraction limit, the resolution of conventional optical microscopes is difficult to reach below 200nm. In recent years, the invention of super-resolution fluorescence microscopy breaks through the optical diffraction limit. These include stimulated emission depletion microscopy (STED), saturated structured illumination microscopy (SSIM), stochastic optical reconstruction microscopy (STORM), and photoactivated localization microscopy (PALM). The three-dimensional spatial resolution of these methods is ...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a microanalysis device, which solves the problem that the existing atomic force microscopy imaging cannot qualitatively analyze the sample, and can obtain the molecular composition information of the sample while obtaining the three-dimensional shape information of the sample, and the resolution can reach 10- 20nm, can be widely used in biomolecular sample analysis, cell membrane structure analysis, cell imaging, nanostructure analysis and nanocatalysis detection, etc.

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