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Enzymatic ethanol determination kit stabilizer

A stabilizer and kit technology, applied in the field of testing, can solve problems such as inconvenience, affecting the accuracy of reagent testing, and failing to meet the needs of actual clinical testing.

Inactive Publication Date: 2015-06-10
CHINA MARITIME POLICE ACADEMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reaction accelerator used in the enzyme analysis method, semicarbazide hydrochloride, will continue to change when exposed to the air, which will affect the detection accuracy of the reagent. When the next sample comes, you must open the cover and put the reagents away before testing, which brings great inconvenience to practical application and cannot meet the actual clinical testing needs.

Method used

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  • Enzymatic ethanol determination kit stabilizer
  • Enzymatic ethanol determination kit stabilizer

Examples

Experimental program
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Effect test

Embodiment 1

[0011] Measure 200ml of dimethyl sulfoxide into the container, heat to 60°C, add 50g of inositol nicotinate while it is hot, and dilute to 1L after complete dissolution.

[0012] Take 1ml of the above-mentioned stabilizer and add it to 99ml of enzymatic ethanol determination reagent 1, and expose it in the air at 2-8°C for 30 days, then test the samples with high and low values ​​of ethanol.

Embodiment 2

[0014] Measure 400ml of dimethyl sulfoxide into the container, heat to 65°C, add 50g of inositol nicotinate while it is hot, and dilute to 1L after complete dissolution.

[0015] Take 1ml of the above-mentioned stabilizer and add it to 99ml of enzymatic ethanol determination reagent 1, and expose it in the air at 2-8°C for 30 days, then test the samples with high and low values ​​of ethanol.

Embodiment 3

[0017] Measure 200ml of dimethyl sulfoxide into the container, heat to 65°C, add 100g of inositol nicotinate while it is hot, and dilute to 1L after complete dissolution.

[0018] Take 1ml of the above-mentioned stabilizer and add it to 99ml of enzymatic ethanol determination reagent 1, and expose it in the air at 2-8°C for 30 days, then test the samples with high and low values ​​of ethanol.

[0019] The test conditions for ethanol samples are as follows: temperature: 30-37°C; light path of cuvette: 1.0cm. The main detection wavelength is 340nm.

[0020] The test method of the ethanol sample is as follows: add reagent 1 to the sample (the calibration tube is used as the sample) and mix well, incubate at 30-37°C for 3-5min, read the absorbance A0, immediately add reagent 2 and mix well, react at 30-37°C for 5min After that, read the absorbance A1, ΔA=A1-A0. The sample volume is 2 μl, the reagent 1 volume is 200 μl, and the reagent 2 volume is 50 μl.

[0021] The ethanol con...

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Abstract

The invention provides an enzymatic ethanol determination kit stabilizer which has a relatively strong stabilizing effect on an enzymatic ethanol determination agent. The enzymatic ethanol determination kit stabilizer comprises inositol niacinate and dimethyl sulfoxide, the use levels of which are respectively 50-100g / L and 200-500ml / L.

Description

technical field [0001] The invention relates to the technical field of testing, in particular to a stabilizer for an enzymatic ethanol assay kit. Background technique [0002] In recent years, criminal cases and traffic accidents caused by drinking have been on the rise. On May 1, 2011, the "Criminal Law Amendment of the People's Republic of China (VIII)" was officially implemented. Drunk driving was held criminally responsible for the crime of dangerous driving. The topic of drunk driving has once again become the focus of attention. [0003] At present, many countries have formulated the relationship between the concentration of ethanol in the blood and the determination of the responsibility of traffic accidents, and established a variety of methods that can detect the content of ethanol in the blood, such as GM / MS method, enzyme end-point colorimetric method, homogeneous enzyme immunoassay method and exhaled ethanol analysis. The GM / MS method is considered as a referen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 王珂王金堂姚旭飞
Owner CHINA MARITIME POLICE ACADEMY
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