A kind of preparation method of special tissue culture medium of dendrobium officinale

A tissue culture, Dendrobium officinale technology, applied in botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of slow growth cycle, artificial seedling, low survival rate, etc. content, the effect of improving the survival rate

Active Publication Date: 2017-11-21
蒙城县望槐信息科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tender stems of a few varieties such as Dendrobium candidum are twisted into spiral or spring-shaped and dried in the sun. The commercial product is called Dendrobium earrings. However, due to the slow growth cycle, it is extremely difficult to survive. The wild Dendrobium candidum is on the verge of extinction, and the survival rate of artificial seedlings is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of preparation method of the special tissue culture liquid of dendrobium officinale proposed by the present invention comprises the following steps:

[0019] S1. Weigh 2.2 parts of potassium iodide, 42 parts of manganese sulfate, 23 parts of zinc sulfate, 72 parts of boric acid, 1 part of sodium molybdate, 0.3 part of copper sulfate, 0.3 part of cobalt chloride in molar parts, add water to dissolve and stir evenly to obtain the first Component, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 25:23;

[0020] S2, take by weighing 33 parts of ferrous sulfate and 59 parts of disodium ethylenediamine tetraacetate by molar parts, add water to dissolve and stir to obtain the second component, wherein the molar volume ratio of ferrous sulfate and the second component (mmol / L) is 33:1.9;

[0021] S3. Weigh 1.6×10 by weight 5 Parts of inositol, 400 parts of niacin, 800 parts of pyridoxine hydrochloride, 170 parts of thiamine hydroch...

Embodiment 2

[0025] A kind of preparation method of the special tissue culture liquid of dendrobium officinale proposed by the present invention comprises the following steps:

[0026] S1. Weigh 2.5 parts of potassium iodide, 40 parts of manganese sulfate, 25 parts of zinc sulfate, 70 parts of boric acid, 1.5 parts of sodium molybdate, 0.1 part of copper sulfate, 0.5 part of cobalt chloride in molar parts, add water to dissolve and stir evenly to obtain the first Components, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 11:12;

[0027] S2, take by weighing 30 parts of ferrous sulfate and 62 parts of disodium ethylenediamine tetraacetate in molar parts, add water to dissolve and stir to obtain the second component, wherein the molar volume ratio of ferrous sulfate to the second component (mmol / L) is 10:0.7;

[0028] S3. Weigh 1.4×10 by weight 5 Parts of inositol, 410 parts of niacin, 780 parts of pyridoxine hydrochloride, 190 parts of thiamine hydr...

Embodiment 3

[0032] A kind of preparation method of the special tissue culture liquid of dendrobium officinale proposed by the present invention comprises the following steps:

[0033] S1. Weigh 2.3 parts of potassium iodide, 41.2 parts of manganese sulfate, 23.7 parts of zinc sulfate, 71.2 parts of boric acid, 1.2 parts of sodium molybdate, 0.22 parts of copper sulfate, 0.36 parts of cobalt chloride in molar parts, add water to dissolve and stir evenly to obtain the first Component, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 2.4:2.35;

[0034] S2, take by weighing 32 parts of ferrous sulfate and 60 parts of disodium ethylenediamine tetraacetate in molar parts, add water to dissolve and stir to obtain the second component, wherein the molar volume ratio of ferrous sulfate to the second component (mmol / L) is 32:1.9;

[0035] S3. Weigh 1.56×10 by weight 5 Parts of inositol, 403 parts of niacin, 795 parts of pyridoxine hydrochloride, 175 parts of ...

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PUM

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Abstract

The invention discloses a preparation method of a special tissue culture solution for Dendrobium officinale, comprising: dissolving potassium iodide, manganese sulfate, zinc sulfate, boric acid, sodium molybdate, copper sulfate and cobalt chloride to obtain a first component; dissolving ferrous sulfate dissolving with disodium EDTA to obtain the second component; dissolving inositol, niacin, pyridoxine hydrochloride, thiamine hydrochloride and glycine to obtain the third component; weighing the peeled potatoes and adding them to the food processor , stir to obtain mashed potatoes, then add coconut water, and mix evenly to obtain material A; add ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, potassium dihydrogen phosphate into water to dissolve to obtain material B; then heat material A, Add material B during the heating process, add the first component, the second component and the third component in turn after stirring evenly, continue to add brown sugar and agar and stir evenly, adjust the pH value to obtain a mixture; Autoclave and cool to room temperature to obtain a special tissue culture solution for Dendrobium officinale.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for preparing a special tissue culture solution for Dendrobium officinale Background technique [0002] Dendrobium is a perennial epiphytic herb of the genus Dendrobium in the family Orchidaceae, and it is a precious traditional Chinese medicinal material commonly used in my country. As early as in "Shen Nong's Materia Medica" and "Compendium of Materia Medica", it was listed as a top-grade medicinal material. Dendrobium is used as medicine with stems, containing various alkaloids such as dendrobine, dendrobium amine, dendrobine, and dendrobium; it is sweet and light in taste, slightly cold in nature; it has the effects of nourishing yin and nourishing stomach, clearing heat and promoting body fluid, mainly for the digestive system, respiratory system, Diseases such as cardiovascular and ophthalmology have obvious therapeutic effects. Modern medicine has prov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨倩倩
Owner 蒙城县望槐信息科技有限责任公司
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