A kind of preparation method of special tissue culture medium of dendrobium officinale
A tissue culture, Dendrobium officinale technology, applied in botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of slow growth cycle, artificial seedling, low survival rate, etc. content, the effect of improving the survival rate
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Embodiment 1
[0018] A kind of preparation method of the special tissue culture liquid of dendrobium officinale proposed by the present invention comprises the following steps:
[0019] S1. Weigh 2.2 parts of potassium iodide, 42 parts of manganese sulfate, 23 parts of zinc sulfate, 72 parts of boric acid, 1 part of sodium molybdate, 0.3 part of copper sulfate, 0.3 part of cobalt chloride in molar parts, add water to dissolve and stir evenly to obtain the first Component, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 25:23;
[0020] S2, take by weighing 33 parts of ferrous sulfate and 59 parts of disodium ethylenediamine tetraacetate by molar parts, add water to dissolve and stir to obtain the second component, wherein the molar volume ratio of ferrous sulfate and the second component (mmol / L) is 33:1.9;
[0021] S3. Weigh 1.6×10 by weight 5 Parts of inositol, 400 parts of niacin, 800 parts of pyridoxine hydrochloride, 170 parts of thiamine hydroch...
Embodiment 2
[0025] A kind of preparation method of the special tissue culture liquid of dendrobium officinale proposed by the present invention comprises the following steps:
[0026] S1. Weigh 2.5 parts of potassium iodide, 40 parts of manganese sulfate, 25 parts of zinc sulfate, 70 parts of boric acid, 1.5 parts of sodium molybdate, 0.1 part of copper sulfate, 0.5 part of cobalt chloride in molar parts, add water to dissolve and stir evenly to obtain the first Components, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 11:12;
[0027] S2, take by weighing 30 parts of ferrous sulfate and 62 parts of disodium ethylenediamine tetraacetate in molar parts, add water to dissolve and stir to obtain the second component, wherein the molar volume ratio of ferrous sulfate to the second component (mmol / L) is 10:0.7;
[0028] S3. Weigh 1.4×10 by weight 5 Parts of inositol, 410 parts of niacin, 780 parts of pyridoxine hydrochloride, 190 parts of thiamine hydr...
Embodiment 3
[0032] A kind of preparation method of the special tissue culture liquid of dendrobium officinale proposed by the present invention comprises the following steps:
[0033] S1. Weigh 2.3 parts of potassium iodide, 41.2 parts of manganese sulfate, 23.7 parts of zinc sulfate, 71.2 parts of boric acid, 1.2 parts of sodium molybdate, 0.22 parts of copper sulfate, 0.36 parts of cobalt chloride in molar parts, add water to dissolve and stir evenly to obtain the first Component, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 2.4:2.35;
[0034] S2, take by weighing 32 parts of ferrous sulfate and 60 parts of disodium ethylenediamine tetraacetate in molar parts, add water to dissolve and stir to obtain the second component, wherein the molar volume ratio of ferrous sulfate to the second component (mmol / L) is 32:1.9;
[0035] S3. Weigh 1.56×10 by weight 5 Parts of inositol, 403 parts of niacin, 795 parts of pyridoxine hydrochloride, 175 parts of ...
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