Application of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis

A diagnostic reagent and a technology for arthritis are applied in the application field of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis, and achieve the effects of strong sensitivity and high specificity

Inactive Publication Date: 2017-05-10
常晓天
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this technology to determine the key enzymes of glucose metabolism and their functions, and to further understand the pathogenesis of RA, is the hotspot of current research, but the relationship between phosphoglycerate kinase 1 and the diagnosis and treatment of RA has not been reported yet.

Method used

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  • Application of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis
  • Application of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis
  • Application of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Discovery and validation of PGK1 as a new blood marker for clinical diagnosis of rheumatoid arthritis

[0065] 1.1 Establishment of type II collagen-induced arthritis (CIA) rat model:

[0066] Male Wistar rats (n=20, each weighing 150-200 g) were obtained from the Animal Science Health Science Experiment Center of Peking University (Beijing, China). The mice were randomly divided into two groups, CIA group and control group. All mice in the experimental groups were intradermally injected with a CFA solution containing 500 μg of bovine collagen II (SIGMA-ALDRICH, USA) at the base of the tail. And after 14 days, use the same method to inject again. The control group was injected with BSA instead of collagen type II by tail root injection in the same manner. CIA rats were clinically scored according to the clinical manifestations of each carpotarsal joint, and the evaluation criteria were as follows: 0=normal, 1=slight edema and erythema, 2=marked edema, and ...

Embodiment 2

[0096] Example 2: Diagnostic reagents and detection methods for clinical diagnosis of rheumatoid arthritis

[0097] Using PGK1 as a blood marker of rheumatoid arthritis, a diagnostic reagent for clinical diagnosis of rheumatoid arthritis was prepared, and the blood of patients was detected. Blood samples were obtained by standard venipuncture from RA patients (n=120, 75 women, aged 20–75 years). Chronic inflammation of the joints for at least 3 years, erythrocyte sedimentation rate (ESR, 19 to 22 mm / hr, mean 44 mm / hr), C-reactive protein (8-196 U / ml, mean 29.1 U / ml) and rheumatoid factor (10.5- 2090U / ml) levels increased. Combination therapy with methotrexate and leflunomide was administered for at least two months. 120 (80 women; 24-58 years, mean 40.2 years) healthy blood donors. Blood samples were put into EDTA-K2 vacuum blood collection tubes.

[0098] The composition of the diagnostic reagent includes: carbonate buffer, PBST washing solution, blocking solution, color ...

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Abstract

The invention discloses a blood marker-PGK1 for rheumatoid arthritis clinical diagnosis, and discloses a reagent for rheumatoid arthritis clinical diagnosis. The reagent comprises a PGK1 antibody, an HRP-IgG antibody and a conventional reagent in a blood diagnosis reagent. The conventional reagent in the blood diagnosis reagent comprises a carbonate buffer solution, PBST washing liquor, confining liquid, a developing solution and a stop solution. The prepared detection reagent has the advantages of being high in sensitivity, high in specificity and the like and is capable of being widely applied to preliminary survey and health examination of clinical rheumatoid arthritis and providing a reliable basis for clinical diagnosis.

Description

technical field [0001] The invention relates to the application of a phosphoglycerate kinase 1 (phosphoglycerate kinase 1, PKG1) in the preparation of a rheumatoid arthritis diagnostic reagent. Background technique [0002] Glucose metabolism mainly refers to the process of glucose degradation, including glycolysis, aerobic oxidation and pentose phosphate pathway metabolism. Glucose metabolism is one of the main metabolic pathways in the body. Glucose metabolism not only provides energy for physical activities, but also mediates various physiological processes through complex signaling networks. Glucose metabolism plays an important role in many diseases such as diabetes, stroke, schizophrenia and substance abuse. [0003] Rheumatoid arthritis (RA) is an autoimmune disease. Recent studies have shown that glucose metabolism plays an important role in the pathogenesis of RA. Henderson. et al. found that the key enzymes of the glycolytic pathway have increased activity leve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/96
CPCG01N33/573G01N2800/102
Inventor 常晓天
Owner 常晓天
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