Application of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis
A diagnostic reagent and a technology for arthritis are applied in the application field of phosphoglycerate kinase 1 in the preparation of diagnostic reagents for rheumatoid arthritis, and achieve the effects of strong sensitivity and high specificity
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Embodiment 1
[0064] Example 1: Discovery and validation of PGK1 as a new blood marker for clinical diagnosis of rheumatoid arthritis
[0065] 1.1 Establishment of type II collagen-induced arthritis (CIA) rat model:
[0066] Male Wistar rats (n=20, each weighing 150-200 g) were obtained from the Animal Science Health Science Experiment Center of Peking University (Beijing, China). The mice were randomly divided into two groups, CIA group and control group. All mice in the experimental groups were intradermally injected with a CFA solution containing 500 μg of bovine collagen II (SIGMA-ALDRICH, USA) at the base of the tail. And after 14 days, use the same method to inject again. The control group was injected with BSA instead of collagen type II by tail root injection in the same manner. CIA rats were clinically scored according to the clinical manifestations of each carpotarsal joint, and the evaluation criteria were as follows: 0=normal, 1=slight edema and erythema, 2=marked edema, and ...
Embodiment 2
[0096] Example 2: Diagnostic reagents and detection methods for clinical diagnosis of rheumatoid arthritis
[0097] Using PGK1 as a blood marker of rheumatoid arthritis, a diagnostic reagent for clinical diagnosis of rheumatoid arthritis was prepared, and the blood of patients was detected. Blood samples were obtained by standard venipuncture from RA patients (n=120, 75 women, aged 20–75 years). Chronic inflammation of the joints for at least 3 years, erythrocyte sedimentation rate (ESR, 19 to 22 mm / hr, mean 44 mm / hr), C-reactive protein (8-196 U / ml, mean 29.1 U / ml) and rheumatoid factor (10.5- 2090U / ml) levels increased. Combination therapy with methotrexate and leflunomide was administered for at least two months. 120 (80 women; 24-58 years, mean 40.2 years) healthy blood donors. Blood samples were put into EDTA-K2 vacuum blood collection tubes.
[0098] The composition of the diagnostic reagent includes: carbonate buffer, PBST washing solution, blocking solution, color ...
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