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In-vitro tooth preservation liquid

A technology of preservation solution and tooth, which is applied in the field of preservation solution of isolated tissues, can solve the problems of less tooth cells, prone to bacterial contamination of isolated teeth, and failure of extracting dental pulp stem cells from isolated teeth, so as to reduce the impact and maintain Effects on cell activity and maintenance of nutrient supply

Active Publication Date: 2015-06-17
ANHUI NEW LIFE STEM CELL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The oral environment is different from other tissue environments. There are a large number of bacteria in the oral cavity, and the types are quite complicated. Improper preservation of isolated teeth is prone to bacterial infection, and the number of cells in teeth is small. Improper preservation of isolated teeth is also prone to dental pulp stem cells. Cases where extraction fails

Method used

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  • In-vitro tooth preservation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Prepare 1L isolated tooth preservation solution. Under sterile conditions, take 0.5L of MEM solution, add 0.5g of penicillin, 0.8g of streptomycin, 0.03g of metronidazole, 0.02g of amphotericin B, and hydroxymethylcellulose 8g, 80ug of insulin, stir to dissolve, add MEM to 1L constant volume, stir evenly, and aseptically dispense the prepared solution into sterilized collection bottles, 25ml per bottle.

Embodiment 2

[0015] Prepare 1L isolated tooth preservation solution, take 0.5L of MEM solution under sterile conditions, add 0.5g of penicillin, 0.8g of streptomycin, 0.03g of metronidazole, 0.02g of amphotericin B, 40g of pluronic F6840g, Dextran sulfate 7g, hydroxymethylcellulose 8g, insulin 80ug, stir to dissolve, add MEM to 1L constant volume, stir evenly, and aseptically dispense the prepared solution into sterilized collection bottles, each bottle 25ml.

[0016] Example of use effect:

[0017] Put 20 isolated healthy teeth in the preservation solution, store them at 2-8 degrees for 120 hours, take them out, and extract the dental pulp stem cells under aseptic operation. The experimental results show that the dental pulp stem cells can be successfully extracted from all 20 teeth.

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Abstract

The invention provides an in-vitro tooth preservation liquid for preserving in-vitro teeth and ensuring the activity of tooth cells. Every 1L of the in-vitro tooth preservation liquid consists of the following components: 0.3-0.6g of penicillin, 0.5-1g of streptomycin, 0.02-0.04g of metronidazole, 0.015-0.025g of amphotericin B, 5-10g of hydroxymethyl cellulose, 50-100mu g of insulin and the balance of MEM. The in-vitro tooth preservation liquid can provide nutrient substances and low-bacterial environments requested by in-vitro teeth, can effectively maintain the activity of cells, and can be used for preserving in-vitro teeth for a long time.

Description

technical field [0001] The invention relates to a preservation solution for isolated tissues, in particular to a preservation solution for isolated teeth. Background technique [0002] Dental defect and tooth loss due to various reasons have become the main diseases that endanger the oral cavity and even human health. At present, various dental materials are mainly used to fill tooth defects to deal with tooth defect diseases. Although it can prevent the continued development of lesions to a certain extent, it cannot solve the fundamental problem. Although there are many solutions to tooth loss, the most effective and fundamental way is tooth regeneration. [0003] With the rapid development of tissue regenerative medicine, it brings hope for the effective treatment of dental defects and missing teeth. At present, many studies have used the interaction of odontogenic epithelium and odontogenic mesenchyme to regenerate tooth tissue. Commonly used odontogenic mesenchyme inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 董成友张路军王颖
Owner ANHUI NEW LIFE STEM CELL TECH CO LTD
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