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A kind of immunoassay method for detecting igm antibody

An immune detection method and antibody technology, which can be applied in the direction of measuring devices, biological tests, material inspection products, etc., can solve the problems of IgG interference detection sensitivity, low specificity, false negatives, etc., to improve detection specificity and detection sensitivity , the effect of reducing interference

Active Publication Date: 2017-01-25
深圳市科润达生物工程有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem that the existing immunoassay method for detecting IgM antibody is not strong in specificity, easily interfered by IgG in the sample to be tested and the detection sensitivity is not high, and prone to false negatives, the purpose of the present invention is to propose a method to solve the aforementioned problems. A kind of immunoassay method for detecting IgM antibody

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  • A kind of immunoassay method for detecting igm antibody
  • A kind of immunoassay method for detecting igm antibody
  • A kind of immunoassay method for detecting igm antibody

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specific Embodiment

[0034] In this example, a rubella virus IgM antibody detection kit (enzyme-linked immunoassay) was used.

[0035] 1.1 Preparation of microplates

[0036] Coating: anti-IgM (specifically goat anti-human IgM, purchased from sigma company) with coating solution (CBS (1L, pH=9.6): Na 2 CO 3 1.59g, NaHCO 3 2.93g) according to the ratio of 1:1000, then use a pipette to add to the microwell plate (a kind of solid phase carrier 1), 100uL per well, and then incubate at 2°C to 8°C for more than or equal to 12 hours, Then wash 3 times with washing liquid;

[0037] Sealing: Pat the washed microwell plate on gauze to dry the residual liquid, then add 200 μL of blocking solution (PBS+0.5% BSA) to each well at 37°C, and block for 2 hours; pour off the blocking solution directly after completion, Just pat dry the residual solution; if it cannot be used immediately, dry the microwell plate at room temperature and humidity below 50%, and then seal it for storage;

[0038] 1.2 Antigen prepa...

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Abstract

The invention discloses an immunodetection method for detecting an IgM antibody, and relates to an immunodetection technology of the IgM antibody. The immunodetection method comprises the following steps: 1, curing processing of anti-IgM; 2, combination of IgM to be detected with the anti-IgM; 3, combination of an antigen and a conjugated monoclonal antibody with the IgM to be detected on a solid phase carrier; and 4, signal detection. The immunodetection method has the advantages of greatly reducing the interference from the IgM to the detection result, reducing the background and false positive rate of the detection, improving the detection specificity, enhancing the detection signal, reducing the false negative rate and improving the detection sensitivity.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to the technical field of IgM antibody immune detection. Background technique [0002] Among the existing immunological detection methods, the immunological detection technology is mainly used to detect the substance to be tested in the sample; in the clinical test, the antibody or antigen in the body fluid is mainly detected through the antigen-antibody reaction. According to different detection principles, immunoassays can be divided into indirect method, sandwich method, competition method and capture method. [0003] In normal serum, IgG is the main component of serum immunoglobulins, accounting for about 75% of the total content of immunoglobulins in serum, while IgM accounts for about 6% of the total amount of immunoglobulins in serum. Specific IgM often coexists with specific IgG, and specific IgG often interferes with the determination of IgM antibodies. Among...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6854
Inventor 张俊卿
Owner 深圳市科润达生物工程有限公司