Preparation method for microencapsulation whey protein hydrolysate with prebiotics and whey protein concentrate being wall materials
A technology of whey protein hydrolysis and whey protein concentrate, applied in whey, dairy products, applications, etc., can solve the problems of low bioacceptability, increased production cost, loss of whey protein hydrolyzate, etc., and improve intestinal The effect of improving the bacterial flora, improving stability, and improving bad flavor
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example 1
[0022] Example 1 Preparation of microencapsulated whey protein hydrolyzate with whey protein concentrate and fructooligosaccharide as wall material
[0023] Preparation of whey protein hydrolyzate: Prepare 5% whey protein concentrate solution with deionized water, heat at 90°C for 5 minutes to passivate possible residual enzymes in whey protein concentrate solution, cool to 55°C, adjust pH to 8, Add trypsin whose mass is 5% of the mass of the substrate, hydrolyze for 8 hours, heat in a boiling water bath for 10 minutes to inactivate the enzyme, then cool in a cold water bath to room temperature, and then centrifuge at 4500g for 10 minutes, and the supernatant obtained is whey protein hydrolyzate. Store at low temperature after freeze-drying.
[0024] Preparation of wall material solution
[0025] Preparation of wall material whey protein concentrate: prepare 10% (w / v) whey protein concentrate solution with deionized water, stir magnetically for 2 hours to fully dissolve, and...
example 2
[0032] Example 2 The preparation of microencapsulated whey protein hydrolyzate with whey protein concentrate and galacto-oligosaccharide as wall material
[0033] The preparation of microencapsulated whey protein hydrolyzate was similar to Example 1. The difference is that fructo-oligosaccharides are replaced by galacto-oligosaccharides. Other steps are the same.
[0034] The bitterness degree of the obtained microencapsulated whey protein hydrolyzate was 4, and the hygroscopicity was 26.23 g water / 100 g solid. Assay method is the same as example 1.
example 3
[0035] Example 3 Preparation of microencapsulated whey protein hydrolyzate with whey protein concentrate, fructo-oligosaccharide and galacto-oligosaccharide mixture as wall material
[0036] The preparation of microencapsulated whey protein hydrolyzate was similar to Example 1. The difference is that the fructo-oligosaccharide is replaced by a mixture of galacto-oligosaccharide and fructo-oligosaccharide, and the mass ratio of the two is 1:1. Other steps are the same.
[0037] The obtained microencapsulated whey protein hydrolyzate had a bitterness value of 4 and a hygroscopicity of 25.98 g water / 100 g solid.
[0038] It can be seen from the above examples that whey protein concentrate and prebiotics in the present invention are effective wall materials for microencapsulated whey protein hydrolyzate. Microencapsulated whey protein hydrolyzate has a significantly reduced level of bitterness that is hardly noticeable. Significantly reduced hygroscopicity and improved stabil...
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