Uses of chlorogenic acid in preparation of drugs treating multidrug resistance of cancer

A multi-drug resistance and chlorogenic acid technology, which is applied in drug combinations, antineoplastic drugs, pharmaceutical formulations, etc., can solve the problems of drug resistance affecting the application effect of drugs, so as to eliminate drug resistance and solve limit problems Effect

Inactive Publication Date: 2015-07-08
刘晓梅
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, both chemotherapy and targeted therapy face a problem, the drug re...

Method used

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  • Uses of chlorogenic acid in preparation of drugs treating multidrug resistance of cancer
  • Uses of chlorogenic acid in preparation of drugs treating multidrug resistance of cancer
  • Uses of chlorogenic acid in preparation of drugs treating multidrug resistance of cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Establishment of drug-resistant tumor cell model

[0046] Take the human breast cancer cell line BCap37 in the logarithmic growth phase and digest it with 0.25% trypsin; resuspend the cells in the complete culture medium and pipette evenly to make a single cell suspension; count the cells and adjust the cell concentration to 2x10 4 cells / ml; 200 μL cell suspension per well was inoculated into a 96-well plate (each well contains about 4000 cells); 2 , saturated humidity, cultivated in a constant temperature incubator at 37°C for 12 hours; added paclitaxel at a concentration of 300nM, and set up 6 replicate wells; 2 , saturated humidity, and cultivated in a constant temperature incubator at 37°C for 72 hours. Using breast cancer cell BCap37 as the parental cell and paclitaxel as the screening drug, the multidrug-resistant cell Bats-72 was successfully established. Bats-72 has broad-spectrum drug resistance, and has strong resistance to chemotherapy drugs such ...

Embodiment 2

[0047] Example 2 Experiment of Inhibitory Rate of Mouse Multidrug-resistant Breast Cancer Model

[0048] (1) Experimental method

[0049] The multidrug-resistant breast cancer model in mice uses C57BL / 6 mice, male, 18-22g. During the experiment, Bats-72 cells in the logarithmic growth phase were taken to make a concentration of 2.5x10 4 / ml of tumor cell suspension, each mouse was inoculated with 0.2ml of tumor fluid in the back of the armpit. Animals were randomly grouped, weighed, and dosed started the day after inoculation. Experimental animals were divided into 9 groups, including negative control group, control group, docetaxel group, chlorogenic acid 5mg / kg, 10mg / kg, 20mg / kg group, docetaxel and chlorogenic acid 5mg / kg, 10mg / kg, 20mg / kg in three dose groups, 20 animals in each group. The administration volume of chlorogenic acid injection was intraperitoneally injected 0.2ml per 10g of mice, once a day, and administered continuously for 24 days. The dose of docetax...

Embodiment 3

[0055] Example 3 Experiment of Inhibitory Rate of Mouse Multidrug-resistant Small Cell Lung Cancer Model

[0056] (1) Experimental method

[0057] Mouse multidrug-resistant small cell lung cancer model, BALB / c mice, male, 18-22g. The model cell used in the experiment is the human small cell lung cancer multidrug-resistant cell line GLC4 induced by mitoxantrone (Mx). During the experiment, GLC4 cells in the logarithmic growth phase were taken to make a concentration of 2.5x10 4 / ml of tumor cell suspension, each mouse was inoculated with 0.2ml of tumor fluid in the back of the armpit. Animals were randomly grouped, weighed, and dosed started the day after inoculation. Experimental animals were divided into 9 groups, including negative control group, control group, doxorubicin group, chlorogenic acid 5mg / kg, 10mg / kg, 20mg / kg group, doxorubicin 60mg / kg and chlorogenic acid 5mg / kg, 10mg / kg, 20mg / kg combined drug in three dose groups, 20 animals in each group. The administrati...

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Abstract

The invention discloses new medicinal uses of chlorogenic acid, especially uses of chlorogenic acid in preparation of drugs treating multidrug resistance of cancer, and provides uses of chlorogenic acid and antitumor drugs in preparation of drugs treating cancer. By inhibiting activity and expression of drug pump protein on surfaces of tumor cells, tumor patients generating multidrug resistance can receive durable and effective treatment. A combination of the chlorogenic acid and a drug-resistant drug is provided, thus alleviating or eliminating drug resistance to multiple drugs for patients, allowing patients with drug resistance to receive durable and effective treatment, and overcoming the limitation problem at present that tumor patients cannot receive durable and effective treatment due to generation of drug resistance. The uses have important meaning in clinical use of antitumor treatment.

Description

technical field [0001] The invention belongs to the new medicinal use of chlorogenic acid, in particular to the use of chlorogenic acid in the preparation of medicines for treating multi-drug resistance of cancer. Background technique [0002] Chemotherapy is the main means of treating cancer most commonly used clinically today, and targeted therapy has also attracted more and more attention in recent years. However, both chemotherapy and targeted therapy face a problem. The drug resistance of tumor cells seriously affects the clinical application effect of drugs. The American Cancer Society estimates that more than 90% of cancer patients die from different degrees of tumor cell drug resistance. Therefore, reducing or reversing the drug resistance of tumor cells, improving the sensitivity of chemotherapy and targeted therapy drugs to tumor cells, and improving positive in treatment. [0003] Studies have shown that the decline in intracellular accumulation is the common ca...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P35/00
Inventor 刘晓梅
Owner 刘晓梅
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