Method used for determining change of podocyte skelemin and marker protein of podocyte skelemin in acute hypertensive renal injury

A technology of skeleton protein and podocytes, which is applied in the field of biomedicine and can solve problems that have not yet been retrieved

Inactive Publication Date: 2015-07-08
李子龙
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the inventors have not retrieved any published reports on the changes of podocyte cytoskeletal proteins and their marker proteins in acute hypertensive kidney injury through the GSK-3β / β-catenin signaling pathway

Method used

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  • Method used for determining change of podocyte skelemin and marker protein of podocyte skelemin in acute hypertensive renal injury
  • Method used for determining change of podocyte skelemin and marker protein of podocyte skelemin in acute hypertensive renal injury
  • Method used for determining change of podocyte skelemin and marker protein of podocyte skelemin in acute hypertensive renal injury

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Embodiment Construction

[0036] The detailed technical route that the present invention provides embodiment has the following steps:

[0037] 1. Animal experiments (see Figure 5 ).

[0038] Preparation of animal models.

[0039] 1. Experimental group:

[0040] 1.1 Normal blood flow model group

[0041] 1.2 Stop blood flow model group

[0042] 1.3 Ischemia-reperfusion model group

[0043] 1.4 Hypertension model group.

[0044] 2. The animal models of different experimental groups were collected by in vivo freezing technology and rapid freezing.

[0045] 3. Morphological analysis and qualitative and quantitative analysis:

[0046] 3.1 Morphological analysis: Immunohistochemistry, laser confocal scanning microscopy, immunoetching, and immunoelectron microscopy were used to perform morphological visualization analysis on the localization of podocyte-related proteins, cytoskeleton proteins, and GSK3β / β-catenin signaling pathway-related proteins.

[0047] 3.2 Qualitative and quantitative analysis: ...

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Abstract

The invention discloses a method used for determining change of podocyte skelemin and marker protein of podocyte skelemin in acute hypertensive renal injury. The method is characterized in comprising steps: mouse acute hypertension models are established; podocyte damage caused by participation of GSK3beta / beta-catenin signal path in acute hypertension is determined, wherein Western blot, and RQ-PCR are used for determining expression of the protein above and mRNA; in vitro culturing of podocyte is carried out so as to study regulation mechanisms of GSK3beta / beta-catenin signal path on podocyte-associated proteins and cytoskeletal proteins, and illustrate start-up functions and core effects of GSK3beta / beta-catenin signal path in acute hypertension podocyte damage further. According to the method, living body freezing technology is combined with freezing substitution for research on podocyte damage when hemodynamics changes, and contribution is provided for development of a method used for preventing and reducing podocyte damage, reducing proteinuria, and providing new therapeutic targets.

Description

technical field [0001] The invention relates to a detection method in biochemistry, in particular to a method for determining the change of podocyte skeleton protein and its marker protein during acute hypertensive kidney injury, and belongs to the technical field of biomedicine. Background technique [0002] Chronic kidney disease (CKD) caused by hypertensive kidney injury has become one of the important causes of endangering public health. Proteinuria is the main clinical manifestation of CKD and an independent risk factor for end stage renal disease (ESRD). Reducing proteinuria can delay the progression of CKD and reduce the morbidity and mortality of ESRD. Therefore, research on how to prevent and reduce the occurrence of proteinuria is of great significance to the prevention and treatment of CKD. [0003] At present, the research focus on the mechanism of proteinuria is podocyte damage. Podocytes are located at the outermost part of the glomerular filtration barrier ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/48C12Q1/02G01N33/68G01N33/573
CPCC12Q1/02C12Q1/48C12Q1/6888C12Q2600/158G01N33/573G01N33/68
Inventor 李子龙
Owner 李子龙
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