High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings

A high-efficiency technology for tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of unfavorable tillering and strong seedlings of tissue cultured seedlings, affect the tillering rate of sugarcane tissue cultured seedlings, and small growth space, so as to achieve excellent multiplication and strong seedlings, and improve Tiller efficiency, the effect of promoting tillering

Inactive Publication Date: 2015-07-15
SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The existing sugarcane tissue culture seedling raising method, the growth space is too small or too large, is not conducive to the tillering and strong seedlings of the tissue culture seedlings, and the control of conditions such as temperature, light conditions and soil water and fertilizer is not in place, which affects the tillering of the sugarcane tissue culture seedlings. important factor in the rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) In April, use river sand as the substrate and use bricks to lay a sand bed with a width of 1.2m. One week before transplanting, disinfect the sand bed with 0.5% carbendazim;

[0028] (2) Using the tissue culture seedlings of Yuncane 05-51 and Yuncane 06-407 as materials, the sugarcane tissue culture seedlings placed in the bottle were hardened for 4 days under the conditions of greenhouse, ventilation and natural light, and then the bottle was opened Cover and harden the seedlings for 2 days;

[0029] (3) Wash the sugarcane tissue cultured seedlings after seedling hardening in step (2), soak them with 0.5% carbendazim for 10 minutes, plant them on the sand bed in step (1) with a plant spacing of 3 cm and a row spacing of 5 cm, and water enough Set root water, then cover with film for 12 days, ventilate properly to prevent mildew, spread a layer of bagasse or peat soil on the sand bed after the seedlings survive, and then thinly apply compound fertilizer 2 times, if ...

Embodiment 2

[0033] (1) In August, use river sand as the substrate and use bricks to lay a sand bed with a width of 1.2m. One week before transplanting, disinfect the sand bed with 0.5% carbendazim;

[0034] (2) Using Yuncane 08-1609 tissue-cultured seedlings as materials, the sugarcane tissue-cultured seedlings placed in the bottle were hardened for 3 days in a greenhouse, ventilated, and natural light conditions, and then the bottle cap was opened for another 3 days. ;

[0035] (3) Wash the sugarcane tissue cultured seedlings after seedling hardening in step (2), soak them with 0.5% carbendazim for 10 minutes, plant them on the sand bed in step (1) with a plant spacing of 2 cm and a row spacing of 6 cm, and water enough Set root water, then cover with film for 15 days, ventilate properly to prevent mildew, if it is in summer, add a shade net, spread a layer of bagasse or peat on the sand bed after the seedlings survive, and then thinly apply compound Fertilize once, if the leaves grow t...

Embodiment 3

[0039] (1) In November, use river sand as the substrate and use bricks to lay a sand bed with a width of 1.2m. One week before transplanting, disinfect the sand bed with 0.5% carbendazim;

[0040] (2) Using Yuncane 08-2800 tissue culture seedlings as materials, the sugarcane tissue culture seedlings placed in the bottle were hardened for 4 days in a greenhouse, ventilated and natural light conditions, and then opened the bottle cap and hardened for 3 days ;

[0041] (3) Wash the sugarcane tissue cultured seedlings after seedling hardening in step (2), soak them with 0.5% carbendazim for 10 minutes, plant them on the sand bed in step (1) with a plant spacing of 3 cm and a row spacing of 6 cm, and water enough Set root water, then cover with film for 10 days, ventilate properly to prevent mildew, if it is in summer, add a shade net, after the seedlings survive, spread a layer of bagasse or peat on the sand bed, and then thinly apply compound Fertilize 2 times, if the leaves gro...

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Abstract

The invention discloses a high-efficiency seedling cultivation method for promoting the premature tillering of sugarcane tissue cultured seedlings, and belongs to the technical field of plant tissue culture. The high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings comprises the following steps of after the disinfection treatment carried out on a sand bed, hardening the sugarcane tissue cultured seedlings and then cleaning and disinfecting the sugarcane tissue cultured seedlings, afterwards, planting the sugarcane tissue cultured seedlings on the sand bed, watering enough rooting water, covering with a film, thinly applying compound fertilizer to obtain plantlets after sprouts survive, pruning the seedlings and then transplanting the seedlings in a potted tray which uses bagasse nutrient soil as a matrix, after the plantlets survive, pruning and hardening the seedlings, then thinly applying the compound fertilizer, pruning the seedlings after leaves grow too luxuriantly, and then carrying out field planting with soil in a big field, so as to complete the seedling cultivation. By using the high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings, the tillering effect of the sugarcane tissue cultured seedlings can be increased furthest; the high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings has excellent effects at the multiplication aspect and the strong seedling aspect of the tissue cultured seedlings, is applicable to the large-scale transplantation of the sugarcane tissue cultured seedlings; not only is the quantity of the sprouts of the sugarcane tissue cultured seedlings increased, but also the survival rate is guaranteed; further, the sprouts grow healthily and strongly; the high-efficiency seedling cultivation method for promoting the premature tillering of the sugarcane tissue cultured seedlings has the characteristics of simple operation, high efficiency and further low cost.

Description

technical field [0001] The invention relates to a high-efficiency seedling raising method for promoting early tillering of sugarcane tissue culture seedlings, belonging to the technical field of plant tissue culture. Background technique [0002] Usually the transplanting of tissue culture seedlings pays attention to the survival rate, but ignores the effect of tillering. Tillers are the branches of grasses and other plants below the ground or close to the ground. If the conditions are right, there will be more tillers. At present, there is no report on the tillering of sugarcane tissue culture seedlings. There is an obvious tillering period in the growth period of sugarcane. The tillering period is the key period for increasing the effective stem per unit area, and it is positively correlated with the yield. The existing methods for raising seedlings of sugarcane tissue culture seedlings have too small or too large growth space, which is not conducive to tillering and stro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
Inventor 吴转娣吴才文刘家勇夏红明覃伟赵丽萍昝逢刚陈学宽赵培方赵俊杨昆姚丽李纯佳朱建荣
Owner SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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