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A method for extracting water-insoluble collagen from pig thoracic aorta

A technology of collagen and thoracic aorta, applied in the direction of animal/human protein, connective tissue peptides, specific peptides, etc., can solve the problems of reducing the safety of collagen materials, not being better utilized, and destroying collagen components, achieving No toxic side effects, good biological activity, and the effect of reducing the degradation rate

Active Publication Date: 2018-02-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of collagen cannot be formed in the water system, and is easily degraded in the in vivo environment
Although collagen materials can be modified by chemical cross-linking, it reduces the safety of collagen materials use
In addition, the alkali treatment or high temperature treatment of the raw materials in conventional extraction will damage the collagen components in the raw materials, and the feature of water solubility makes the purification process cumbersome
[0005] my country is a large pig farmer. In addition to supplying daily food needs, pig skin has been recycled to extract collagen, and the small intestine is used to extract heparin sodium with anticoagulant activity. pollute the environment

Method used

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  • A method for extracting water-insoluble collagen from pig thoracic aorta
  • A method for extracting water-insoluble collagen from pig thoracic aorta

Examples

Experimental program
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Effect test

Embodiment 1

[0016] A method for extracting water-insoluble collagen from pig thoracic aorta, comprising the steps of:

[0017] (1) Pretreatment: adopt quarantine-qualified pigs, open the cavity aseptically, take the thoracic aorta, remove the surface connective tissue, rinse with sterile water, freeze-dry, and pulverize to 100 mesh;

[0018] (2) Degreasing: Place the crushed thoracic aorta in step (1) in acetone reagent, stir at -20°C for 12 hours, centrifuge at 5000r / min for 30 minutes, remove the milky white supernatant, and replace it with a new one. Acetone reagent Repeat the above degreasing operation until the supernatant is clear and transparent, remove the supernatant, and place the lower product in a vacuum drying oven to air dry;

[0019] (3) Impurity removal: Add 4M guanidine hydrochloride solution 10 times the volume of the product weight to the lower layer product obtained in step (2), stir at 4°C for 72 hours, centrifuge at 8000r / min for 30min, and remove the supernatant Af...

Embodiment 2

[0026] A method for extracting water-insoluble collagen from pig thoracic aorta, comprising the steps of:

[0027] (1) Pretreatment: adopt quarantine-qualified pigs, open the cavity aseptically, take the thoracic aorta, remove the surface connective tissue, rinse with sterile water, freeze-dry, and pulverize to 70 mesh;

[0028] (2) Degreasing: Place the crushed thoracic aorta in step (1) in acetone reagent, stir at -20°C for 12 hours, centrifuge at 5000r / min for 30 minutes, remove the milky white supernatant, and replace it with a new one. Acetone reagent Repeat the above degreasing operation until the supernatant is clear and transparent, remove the supernatant, and place the lower product in a vacuum drying oven to air dry;

[0029] (3) Impurity removal: Add 4M guanidine hydrochloride solution of 12 times the volume of the product weight to the lower layer product obtained in step (2), stir at 4°C for 72 hours, centrifuge at 8000r / min for 30min, and remove the supernatant ...

Embodiment 3

[0036] A method for extracting water-insoluble collagen from pig thoracic aorta, comprising the steps of:

[0037] (1) Pretreatment: adopt quarantine-qualified pigs, open the cavity aseptically, take the thoracic aorta, remove the surface connective tissue, rinse with sterile water, freeze-dry, and pulverize to 30 mesh;

[0038] (2) Degreasing: Place the crushed thoracic aorta in step (1) in acetone reagent, stir at -20°C for 12 hours, centrifuge at 5000r / min for 30 minutes, remove the milky white supernatant, and replace it with a new one. Acetone reagent Repeat the above degreasing operation until the supernatant is clear and transparent, remove the supernatant, and place the lower product in a vacuum drying oven to air dry;

[0039] (3) Impurity removal: Add 4M guanidine hydrochloride solution 15 times the volume of the product weight to the lower layer product obtained in step (2), stir at 4°C for 72 hours, centrifuge at 8000r / min for 30min, and remove the supernatant Aft...

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Abstract

The invention relates to a method for preparing water-insoluble collagen by using porcine thoracic aorta as raw material through degreasing, impurity removal, enzymatic hydrolysis, salting out, dialysis and other technical steps. The process steps of the method are simple, safe and easy to operate, the cost is low, and the obtained product has high purity. Compared with the water-soluble collagen, the degradation rate of the water-insoluble collagen obtained in the present invention is reduced in vivo, and the coagulation speed is faster, and can be used for wound hemostasis or preparation of other medical biomaterials.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a method for preparing water-insoluble collagen by using porcine thoracic aorta as a raw material through degreasing, impurity removal, enzymatic hydrolysis, salting out, dialysis and other process steps. Background technique [0002] Collagen mainly exists in the connective tissue of animals. It is the most abundant and widely distributed functional protein in mammals. It plays a role in supporting, protecting, combining and separating the body and organs. Maturation, blood coagulation, wound healing, intercellular signaling, delivery of biological factors, and aging all play pivotal roles. [0003] Collagen is widely used in cosmetics, food, and health care products in the industry. In addition, due to the low immunogenicity of collagen, hemostatic effect, can promote cell proliferation and cell growth, biodegradable and no toxic side effects of degradation products, etc. , ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
Inventor 陈敬华付海田邓超
Owner JIANGNAN UNIV
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