A method for producing glycyrrhetinic acid by microbial transformation and its culture medium

A technology for transforming culture medium and microbial transformation, which is applied in the field of microbial transformation and production of glycyrrhetic acid, the method and the culture medium thereof, can solve problems such as low transformation rate, and achieve the effects of low cost, simple culture process and mild reaction conditions

Active Publication Date: 2018-04-27
国药集团健康产业研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for the production of glycyrrhetinic acid by microbial transformation in view of the low conversion rate of glycyrrhetinic acid produced by microbial conversion of glycyrrhizic acid at present. High yield, suitable for industrial production of glycyrrhetinic acid

Method used

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  • A method for producing glycyrrhetinic acid by microbial transformation and its culture medium
  • A method for producing glycyrrhetinic acid by microbial transformation and its culture medium
  • A method for producing glycyrrhetinic acid by microbial transformation and its culture medium

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Experimental program
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Effect test

Embodiment 1

[0058] Slant culture: inoculate the strain on the slant medium, and culture at 28°C for 3-7 days.

[0059] Seed culture: use the obtained slant culture to inoculate into the seed medium, shake and culture at 28° C. and 240 rpm for 48 hours to obtain the seed liquid. This seed solution was used in Examples 1-27.

[0060] Wherein, the seed medium is: PDA liquid medium.

[0061] Seed solution is inoculated in the Erlenmeyer flask containing transformation medium with the inoculum size of 10% (v / v), the composition of transformation medium: glycyrrhizic acid 5 grams / liter, MgSO 4 ·7H 2 O0.5 g / L, NaNO 3 5 g / L, FeSO 4 ·7H 2 O0.01 g / L, KH 2 PO 4 1 g / L, pH 5.0. Automatically control the fermentation temperature at 25°C and shake at 200 rpm for 144 hours, and the molar production rate of glycyrrhetinic acid is 82.8%.

Embodiment 2

[0063] Seed solution is inoculated in the Erlenmeyer flask containing transformation medium with the inoculum size of 10% (v / v), the composition of transformation medium: glycyrrhizic acid 5 grams / liter, MgSO 4 ·7H 2 O0.5 g / L, NaNO 3 5 g / L, FeSO 4 ·7H 2 O0.01 g / L, KH 2 PO 4 1 g / L, pH 5.0. Automatically control the fermentation temperature at 30°C and shake at 200 rpm for 144 hours, and the molar production rate of glycyrrhetinic acid is 96.5%.

Embodiment 3

[0065] Seed solution is inoculated in the Erlenmeyer flask containing transformation medium with the inoculum size of 10% (v / v), the composition of transformation medium: glycyrrhizic acid 5 grams / liter, MgSO 4 ·7H 2 O0.5 g / L, NaNO 3 5 g / L, FeSO 4 ·7H 2 O0.01 g / L, KH 2 PO 4 1 g / L, pH 5.0. The fermentation temperature was automatically controlled at 37°C, and the shaking culture at 200rpm was carried out for 144h, and the molar production rate of glycyrrhetinic acid was 69.8%.

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Abstract

The invention discloses a method for microbial transformation and production of glycyrrhetinic acid and a culture medium thereof. The method comprises the following steps: insert the seed solution of Aspergillus ustus into the transformation medium according to the inoculation amount of 3%-20% by volume, and cultivate it at 25°C-37°C for 48 hours-240 hours, wherein the transformation culture The base includes the following components: glycyrrhizic acid 1-15 g / L, NaNO3 1-10 g / L, pH 3-7. The method uses Aspergillus pyrorrhizinum to convert glycyrrhetinic acid for the first time, the final conversion product is single, and the molar conversion rate of glycyrrhetinic acid is as high as 96.5%, which is suitable for industrial production of glycyrrhetinic acid.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing glycyrrhetinic acid by microbial transformation and a culture medium thereof. Background technique [0002] As a commonly used traditional Chinese medicine, licorice has been accepted and used by people. Its main components are: glycyrrhizic acid, glycyrrhizin, licorice flavonoids, and bistanol. It has detoxification, anti-inflammatory, antitussive, anti-tumor, anti-ulcer and antibacterial effects. Glycyrrhizic acid (Glycyrrhizic acid, GL) is the main active substance in licorice. Glycyrrhizic acid and its series of products have different pharmacological activities, such as liver protection, anti-AIDS, anti-virus and anti-cancer effects. In addition, glycyrrhizic acid is also widely used in food because of its high sweetness and special flavor, and is often used as a food additive and spice base. [0003] On the other hand, glycyrrhizic acid is also used as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00C12R1/70
Inventor 胡海峰魏婷婷
Owner 国药集团健康产业研究院有限公司
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