Method for purifying caffeoyl guinic acid compounds in purple sweet potato stem and leaf through macro-porous resin
A technology of caffeoylquinic acid and macroporous resin, which is applied in the field of extraction of caffeoylquinic acid compounds, can solve the problems of expanding the natural source of caffeoylquinic acid compounds, less development and utilization of cassava leaves, waste of resources and the like
Inactive Publication Date: 2015-08-12
HUNAN AGRICULTURAL UNIV
View PDF3 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] The purpose of the embodiments of the present invention is to provide a method for purifying caffeoylquinic acid compounds in purple sweet potato stems and leaves with macroporous resin, aiming at solving the problem of waste of resources caused by less development and utilization of potato leaves, and at the same time Expanding Natural Sources of Caffeoylquinic Acids
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0031] 1. Detection of caffeoylquinic acid compounds in purple sweet potato stems and leaves
[0032] Accurately weigh 6.17 mg of a standard chlorogenic acid with a purity of 98% in a 25 mL volumetric flask, and dilute to volume with 50% ethanol. Precisely draw 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6mL of the above reference solution into a 10mL volumetric flask, add 5ml of distilled water, shake well, add 0.5mol / L Folin-C solution , after shaking well, add 1.5ml of 20% sodium carbonate solution, constant volume, and react at 25°C for 2 hours to obtain standard solution of different concentrations, adjust to zero with the sample blank, measure the absorbance at 760nm wavelength, take the standard substance mass (mg) is the abscissa, the absorbance is the ordinate, the linear regression equation y=4.0976x+0.001, R2=0.993.
[0033] Weigh 1.0g of purple sweet potato stems and leaves of different varieties, add 100ml of 60% ethanol solution, put it in a water bath at 90°C ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for purifying caffeoyl guinic acid compounds in purple sweet potato stem and leaf through macro-porous resin. The method comprises the following steps: drying purple sweet potato stem and leaf at 60DEG C, crushing, extracting the above obtained dried raw material at 80-100DEG C for 0.5-1.5h, filtering, extracting obtained raw material residues under same conditions through a same extraction process once, mixing the above obtained extraction liquids, carrying out vacuum concentration on the above obtained extraction liquid mixture at 55DEG C to recover ethanol in order to obtain a purple sweet potato stem and leaf extraction liquid, adjusting the extract liquid to the content of the caffeoyl guinic acid compounds of 1-3mg / ml and the pH value of 2-3, and adding the obtained sample to macro-porous resin according to an adding flow velocity of 2-3BV / h and a sample volume of 5BV or less; and eluting by adopting 5-6BV of water until colorlessness, sequentially eluting by using different concentrations of ethanol according to a flow velocity of 2-3BV / h, respectively concentrating the above obtained eluates, and freeze-drying the eluates to obtain the following two products with different specifications: a 20% ethanol elution product with the content of the caffeoyl guinic acid compounds of above 20%, and a 40-70% ethanol elution product with the content of the caffeoyl guinic acid compounds of above 60%. The method enlarges the natural source of the caffeoyl guinic acid compounds.
Description
technical field [0001] The invention belongs to the field of extraction of caffeoylquinic acid compounds, in particular to a method for purifying caffeoylquinic acid compounds in purple sweet potato stems and leaves with a macroporous resin. Background technique [0002] Caffeoylquinic acids are a class of phenylpropanoids synthesized by plants through the intermediates of the pentose phosphate pathway during aerobic respiration. They are widely found in plants and have significant antioxidant and pharmacological activities, such as Anti-inflammatory, anti-HIV activity, bacteriostasis, inhibition of various enzyme activities, liver protection, inhibition of platelet aggregation, etc., are active ingredients of many medicinal materials, and have become important indicators for the quality control of some traditional Chinese medicine preparations. In addition to medicinal use, it can also be used as an antioxidant in the food industry. It also has the effect of increasing flav...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
CPCC07C67/48C07C69/732
Inventor 陆英李觅路陈金华秦玉芝刘毅
Owner HUNAN AGRICULTURAL UNIV