Nucleotide sequence for preventing infection of toxoplasmas and application of nucleotide sequence

A nucleotide sequence and infection prevention technology, applied in anti-infective drugs, medical preparations containing active ingredients, using vectors to introduce foreign genetic material, etc., can solve problems such as economic losses in animal husbandry

Inactive Publication Date: 2015-08-12
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, one-third of the world's population is infected with Toxoplasma gondii, and most people are invisible infections, but the infection of Toxoplasma gondii in patients with low immunity or immunodeficiency (HIV carriers, organ transplants, etc.) can cause serious consequences

Method used

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  • Nucleotide sequence for preventing infection of toxoplasmas and application of nucleotide sequence
  • Nucleotide sequence for preventing infection of toxoplasmas and application of nucleotide sequence

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Embodiment 1

[0021] (1) Preparation of Toxoplasma gondii RH strain pVAX-profilin

[0022] 1. Take out the Toxoplasma gondii RH strain from liquid nitrogen, thaw in a water bath at 37°C, and inject Kunming mice into the abdominal cavity. Centrifuge at 6000rpm for 10min, discard the supernatant, and store the pellet in a -80°C refrigerator for RNA extraction. according to The operating instructions of Total RNA Kit I (purchased from Omega Company) were used to extract recombinant total RNA.

[0023] 2. Amplification of the Profilin gene:

[0024] 2.1 Primer design

[0025] Referring to the sequence of the profilin gene of Toxoplasma gondii, the primer sequences for amplifying the profilin gene of Toxoplasma gondii were designed, including:

[0026] Upstream: 5'-CGG GGTACC ATGTCCGACTGGGACCCTGTTGTCAAGG-3',

[0027] Downstream: 5'-CCG GAATTC TTAGTACCCAGACTGGTGAAGATACTCG-3',

[0028] Wherein, the underlined parts are Kpn I and EcoR I restriction sites respectively.

[0029] 2.2PCR amp...

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Abstract

The invention provides a nucleotide sequence for preventing infection of toxoplasmas. The nucleotide sequence contains any one group of the following nucleotide sequences: (1) a nucleotide sequence of profilin; (2) nucleotide sequences of profilin, rop16, rop18, MIC6 and CDPK3; (3) nucleotide sequences of profilin and IL15; (4) nucleotide sequences of rop16, rop18, MIC6 and CDPK3; (5) complementary sequences of the nucleotide sequences from (1) to (4); and (6) nucleotide sequences prepared through carrying out substitution, deletion, addition and modification on nucleotide sequences from (1) to (5). By virtue of a pVAX-profilin vaccine and a combined vaccine, the generation of brain cysts of the toxoplasmas can be effectively inhibited, and a relatively good immune effect is achieved.

Description

technical field [0001] The present invention relates to the nucleotide sequence and its application for the prevention of Toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii is a globally distributed Apicomplexa that can invade almost all nucleated warm-blooded animals including humans. According to reports, one-third of the world's population is infected with Toxoplasma gondii, and most people are invisible infections, but the infection of Toxoplasma gondii in patients with low immunity or immunodeficiency (HIV carriers, organ transplants, etc.) can cause serious consequences . Toxoplasma gondii acute outbreaks can also cause serious economic losses to animal husbandry. There is still no ideal drug for the treatment of toxoplasmosis, so it is particularly important to develop a safe and effective anti-toxoplasma vaccine in the prevention and treatment of toxoplasmosis. Contents of the invention [0003] In order to solve the problems in the pri...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/70C07K14/45A61K48/00A61K31/711A61K38/16A61P33/02
Inventor 黄思扬高琦张念章徐民俊翁亚彪朱兴全
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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