Nondestructive testing method of content of fatty acid in peony seeds

A technology for non-destructive testing and peony seeds, applied in the field of testing, can solve problems such as inability to achieve non-destructive testing of seeds, and achieve the effects of rapid non-destructive testing, close prediction accuracy, and easy operation

Inactive Publication Date: 2015-08-26
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0004] The detection effect of NIRS varies with the sample material. Seeds such as rapeseed, soybean, and corn are relatively small and regular in size, while other larger seeds such as cottonseed and Camellia oleifera are often measured with powder or peeled kernels, which cannot be achieved. Non-destructive testing of seeds
However, there is no relevant report on NIRS detection technology for peony seeds

Method used

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  • Nondestructive testing method of content of fatty acid in peony seeds

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Embodiment 1

[0037] Step 1: Harvest mature peony seeds and dry them at 60°C for 8 hours for later use. Among them, there are 97 mature seeds of peony, including 26 cultivated peony (P.suffruticosa), 19 Fengdan peony (P.ostii), 46 purple spot peony (P.rockii), and ovale peony (P.qiui) 3 , and 3 parts of peony (P.lactiflora). The selected materials cover the main germplasm types of peony cultivated in my country.

[0038] Step 2, weigh 20g of each sample and put it into the automatic rotating cup of ANTARIS Fourier transform near-infrared scanner (Thermo Nicolet Co., USA). -1 Range scans 64 times. Each sample was filled three times, and the average spectral value was taken.

[0039] The third step is to measure the fatty acid content of each sample by gas chromatography, that is, the chemical value, and finally use TQ Analyst V7.2 software to establish a mathematical model between the spectral value and the chemical value.

[0040] Wherein the step of gas chromatography comprises:

[00...

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Abstract

The invention provides a nondestructive testing method of the content of fatty acid in peony seeds. The method comprises the steps that first, ripe peony seeds are collected and are dried at 60 DEG C to serve as samples; second, the peony seed samples are respectively placed into an automatic rotating cup of a Fourier near-infrared scanner, and are scanned for 64 times within the range from 4000 cm<-1> to 10000 cm <-1>, each sample is repeatedly loaded for three times, and the average value is obtained; third, the content, namely the chemical value, of fatty acid in each sample is determined with a gas chromatography method, and then, a mathematical model is built according to spectral values and the chemical values; fourth, unknown peony seed samples are tested with the model. With the nondestructive testing method of the content of fatty acid in peony seeds, the content of palmitic acid, oleic acid, linoleic acid and linolenic acid in peony seeds can be rapidly tested in a nondestructive mode; operation is simple and convenient, and because model building is carried out on the basis of the chemical values and the spectral values of various peony seeds, the reliable mathematical model is obtained; a prediction result is high in accuracy, the accuracy of model prediction is close to the true value of the background technology.

Description

technical field [0001] The invention belongs to the field of testing, and in particular relates to a method for measuring fatty acid content by using near-infrared spectroscopy. Background technique [0002] Peony (Paeonia suffruticosa Andr.) is a famous and precious flower specially produced in China. It has a long history of cultivation and profound cultural heritage, and is more and more loved by people all over the world. In recent years, the value of peony seed oil has been widely recognized. The high oil content of the seeds, especially the high content of α-linolenic acid, makes the peony seed oil have high nutritional value, and the yield of peony seeds is also high. New oil crops have broad market development potential. For a long time, the oil content and oil detection methods of plant seeds have mainly relied on Soxhlet extraction, gas chromatography and other methods. Using these traditional methods requires peeling and crushing peony seeds and then extracting t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563
Inventor 成仿云崔虎亮钟原
Owner BEIJING FORESTRY UNIVERSITY
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