Application of chromogenic method based on nano-gold aggregation in immunoassay

An immune detection and nano-gold technology, which is applied in the field of chemical detection, can solve the problems of low sensitivity, achieve the effects of shortening the reaction time, rapid and stable color development, and improving stability

Inactive Publication Date: 2017-11-03
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the color mechanism of this detection technology itself, the sensitivity is low, and it is not suitable for detecting very low concentrations of analytes

Method used

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  • Application of chromogenic method based on nano-gold aggregation in immunoassay
  • Application of chromogenic method based on nano-gold aggregation in immunoassay

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Experimental program
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Effect test

Embodiment 1

[0022] 1. Preparation of functionalized gold nanoparticles

[0023] 1. Select a compound containing a terminal alkynyl group to modify gold nanoparticles. The present invention takes compound 1 as an example, and its structural formula is:

[0024]

[0025] The target molecule containing a terminal alkynyl group contains a disulfide bond, and can be combined with nano gold through a gold-sulfur bond.

[0026] 2. Select gold nanoparticles with a diameter of 5nm to 500nm: take 6mL of gold nanoparticles solution, add 100 μL of compound 1 (1mM, dissolved in ethanol) while stirring, and adjust the pH of the solution to alkaline with NaOH to ensure good dispersion of gold nanoparticles .

[0027] 3. The mixed solution was placed on a shaker and mixed for 24 hours, centrifuged and washed with a high-speed centrifuge to remove excess compound 1 in the solution.

[0028] 4. Redisperse with deionized water to obtain functionalized gold nanoparticles, and store it as a chromogenic s...

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Abstract

The application of the chromogenic method based on nano-gold aggregation in immunoassay is to modify the nano-gold with a compound containing terminal alkynyl groups, and the modified functionalized nano-gold has a positive response to silver ions or monovalent copper ions , and then add silver ions or monovalent copper ions to make the functionalized gold nanoparticles produce a color reaction due to different degrees of aggregation: the original red of the nano gold solution changes to blue; The generated chromogenic reaction is applied to immunoassay. Due to the different aggregation degree of nano gold, its ultraviolet-visible absorption spectrum changes. Therefore, the changing absorption spectrum can be measured by the instrument to realize quantitative detection in immunoassay. The invention can not only observe with the naked eye but also characterize the ultraviolet-visible absorption spectrum of the color reaction through an instrument, and is of positive help to chemical detection, especially immunological detection.

Description

technical field [0001] The invention relates to the technical field of chemical detection, and relates to the use of silver ions or monovalent copper ions to cause the aggregation of functionalized nano-gold as a color reaction, and the color change is applied to immune detection. Specifically, organic molecules containing terminal alkynyl groups are used to functionalize gold nanoparticles. The reaction of organic molecules containing terminal alkynyl groups with silver or monovalent copper ions will cause the aggregation of functionalized gold nanoparticles, and the color of the solution will change. And this chromogenic reaction was applied to immunoassay. Background technique [0002] Nanomaterials have several special properties such as surface effect, quantum size effect, and small size effect. The surface plasmon resonance of gold nanoparticles will change with the distance between particles. When the gold nanoparticles in the solution change from dispersed to aggreg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N33/53G01N33/68
Inventor 马巍于汝佳刘晓院彭茂潘韩焕兴龙亿涛
Owner EAST CHINA UNIV OF SCI & TECH
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