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Application of color development method based on nanogold aggregation to immunodetection

An immune detection and nano-gold technology, which is applied in the field of chemical detection, can solve the problems of low sensitivity, achieve the effects of shortening the reaction time, rapid and stable color development, and improving stability

Inactive Publication Date: 2015-08-26
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the color mechanism of this detection technology itself, the sensitivity is low, and it is not suitable for detecting very low concentrations of analytes

Method used

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  • Application of color development method based on nanogold aggregation to immunodetection

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Experimental program
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Embodiment 1

[0022] 1. Preparation of functionalized gold nanoparticles

[0023] 1. Select a compound containing a terminal alkynyl group to modify gold nanoparticles. The present invention takes compound 1 as an example, and its structural formula is:

[0024] ;

[0025] The target molecule containing a terminal alkynyl group contains a disulfide bond, and can be combined with nano gold through a gold-sulfur bond.

[0026] 2. Select gold nanoparticles with a diameter of 5nm to 500nm: Take 6mL of gold nanoparticles solution, add 100μL of compound 1 (1mM, dissolved in ethanol) while stirring, adjust the pH of the solution to alkaline with NaOH to ensure good dispersion of gold nanoparticles .

[0027] 3. The mixed solution was placed on a shaker and mixed for 24 hours, centrifuged and washed with a high-speed centrifuge to remove excess compound 1 in the solution.

[0028] 4. Redisperse with deionized water to obtain functionalized gold nanoparticles, and store it as a chromogenic sol...

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Abstract

The invention discloses an application of a color development method based on nanogold aggregation to immunodetection. A compound containing terminal alkynyl is used for modifying nanogold, the modified functional nanogold can actively respond to silver ions or cuprous ions, and silver ions or cuprous ions are added, so that the modified functional nanogold produces a color development reaction due to different aggregation degrees, that is to say, a nanogold solution becomes blue from originally red; the color development reaction produced by the functional nanogold due to different aggregation degrees is applied to the immunodetection, and ultraviolet-visible absorption spectrums have a change due to different aggregation degrees of the nanogold, so that the changeable absorption spectrums can be measured through an instrument and quantitative detection in the immunodetection is realized. The ultraviolet-visible absorption spectrums in the color development reaction can be not only observed through naked eyes but also represented through the instrument, and the color development method has positive help for chemical detection, especially the immunodetection.

Description

technical field [0001] The invention relates to the technical field of chemical detection, and relates to the use of silver ions or monovalent copper ions to cause the aggregation of functionalized nano-gold as a color reaction, and the color change is applied to immune detection. Specifically, organic molecules containing terminal alkynyl groups are used to functionalize gold nanoparticles. The reaction of organic molecules containing terminal alkynyl groups with silver or monovalent copper ions will cause the aggregation of functionalized gold nanoparticles, and the color of the solution will change. And this chromogenic reaction was applied to immunoassay. Background technique [0002] Nanomaterials have several special properties such as surface effect, quantum size effect, and small size effect. The surface plasmon resonance of gold nanoparticles will change with the distance between particles. When the gold nanoparticles in the solution change from dispersed to aggreg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/53G01N33/68
Inventor 马巍于汝佳刘晓院彭茂潘韩焕兴龙亿涛
Owner EAST CHINA UNIV OF SCI & TECH
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