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A kind of bletilla striata tissue culture medium and its preparation method

A technology of tissue culture medium and culture medium, which is applied in the field of plant tissue culture, can solve the problems of small protocorms, long culture period, and affecting the survival rate of seedling hardening cultivation, etc., and achieve the goal of large protocorms, rich nutrient components, and reduced production costs Effect

Active Publication Date: 2017-06-16
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The related technology of bletilla striata tissue culture has been mature, but due to the long culture cycle, the protocorms cultivated are small, which affects the survival rate of seedling hardening and cultivation in the later stage

Method used

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  • A kind of bletilla striata tissue culture medium and its preparation method
  • A kind of bletilla striata tissue culture medium and its preparation method
  • A kind of bletilla striata tissue culture medium and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Calculated by preparing 10L bletilla striata tissue culture medium, its preparation method is as follows:

[0031] 1. Weigh 750g of peeled potatoes, cut the potatoes into small pieces of about 1cm×1cm, add 1L of deionized water to the blender, stir for 15min, and mash the potatoes;

[0032] 2. Weigh 500g of peeled bananas, cut them into small pieces of about 1cm×1cm, add 1L of deionized water to the blender, stir for 15min, and mash the bananas;

[0033] 3. Take two parts of 250g sucrose and 60g agar powder and add them to 10L container A (preparation of seed germination medium) and 10L container B (preparation of strong seedling medium) respectively;

[0034] 4. Pour the mashed potatoes prepared in step 1 into container A, and transfer the mashed bananas prepared in step 2 into container B;

[0035] 5. Measure 250mLMS of 20 times the mother liquor of macroelements, 10mLMS of 1000 times of mother liquor of trace elements, 1000 times of mother liquor of 10mLMS of organi...

Embodiment 2

[0038] Press medium preparation method among the embodiment 1, agar powder addition is identical with example 1, in two stages of seed germination and strong seedling rooting culture, screen with medium combinations listed in table 1 respectively, to optimize optimum medium Combination recipe. Table 1 explains: ①The optimization and screening of medium formula is mainly to design different basal medium, hormone concentration, natural additives, etc. to optimize the design of the medium; ②N6+1 / 2MS in Table 1 is the macroelement of N6 and 1 The combination of trace elements and organic matter of / 2MS; ③The material used in the screening of the seed germination medium in this example is Bletilla striata capsule, the Bletilla striata capsule is subjected to surface disinfection treatment, cut open under aseptic conditions to obtain seeds, and sow through aseptic operation In the seed germination medium of table 1; The material used in the screening of the strong seedling rooting m...

Embodiment 3

[0043] Calculated by preparing 10L bletilla striata tissue culture medium, its preparation method is as follows:

[0044] 1. Weigh 1000g of peeled potatoes, cut the potatoes into small pieces of about 1cm×1cm, add 1L of deionized water to the blender, stir for 15min, and mash the potatoes;

[0045] 2. Weigh 1000g of peeled bananas, cut them into small pieces of about 1cm×1cm, add 1L of deionized water to the blender, stir for 15min, and mash the bananas;

[0046]3. Take two parts of 250g sucrose and 60g agar powder and add them to 10L container A (preparation of seed germination medium) and 10L container B (preparation of strong seedling medium) respectively;

[0047] 4. Pour the mashed potatoes made in step 1 into container A, and transfer the mashed bananas made in step 2 to container B; measure 20 times the mother liquor of 250mLMS macroelements, 1000 times mother liquor of 10mLMS trace elements, and 1000 times mother liquor of 10mLMS organic matter. Double the mother liqu...

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Abstract

The invention discloses a Bletilla striata tissue culture medium and a preparing method thereof. The Bletilla striata tissue culture medium comprises a seed germination culture medium and a strong seedling culture medium; the seed germination culture medium is composed of 1 / 2 MS macronutrients, 0.5mg / L to 1.0mg / L of 6-benzylaminopurine, 0.2mg / L to 1.0mg / L of 1-naphthaleneacetic acid, 50g / L to 100g / L of potato, 25g / L of cane sugar, and 6.0g / L of agar. The strong seedling culture medium is composed of N6 macronutrients, MS organics, MS trace elements, 0.1mg / L to 1.0mg / L of 1-naphthaleneacetic acid, 50g / L to 100mg / L of banana slurry, 25g / L cane sugar, and 6.0g / L of agar. Compared with the prior art, the Bletilla striata tissue culture medium allows the formation rate of pseudobulbs of Bletilla striata to reach 95% and emergence rate of tissue culture seedlings to exceed 98%, with big protocorm-like bodies and high survival rate of transplanted and exercised seedlings; meanwhile, the Bletilla striata tissue culture medium and the preparing method thereof have the advantages that the cultivation period of Bletilla striata is greatly shortened and the production cost is lowered.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a bletilla striata tissue culture medium and a preparation method thereof. Background technique [0002] Bletilla striata, also known as Baiji, is a perennial herb belonging to the family of Orchidaceae. The dried tubers are used for medicinal purposes, and have the effects of astringent, hemostasis, swelling and granulation. Clinically, it is often used to treat diseases such as tuberculosis hemoptysis, gastric ulcer hematemesis, blood in stool and traumatic bleeding. The tubers of Bletilla striata are rich in polysaccharides of Bletilla striata, the main component of which is glucomannan, which is widely used in food industry, medical and sanitary materials and daily chemical industry. [0003] The seeds of Bletilla striata are as small as dust, and it is extremely difficult to germinate in the natural environment. With the increase of demand, the wild resource...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 席刚俊徐超史俊汪善峰李警保
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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