A method for tissue culture and rapid propagation of plant regeneration induced by stem segments of North American Sequoia

Abstract

The invention discloses a sequoia sempervirens steam section induction plant regeneration tissue culture rapid propagation method, and belongs to the technical field of forest wood tissue culture breeding. The method belongs to a propagation method characterized in that the screened sequoia sempervirens high-quality plants are used as female parents; the annual stems with buds are selected to be used as explants for inducing normal bud germination; then, aseptic seedlings are used for induction to generate adventitious buds; then, the adventitious buds are used for inducing regeneration plantsto root for seedling domestication and transplanting potting. The sequoia sempervirens fast breeding technology is used for mass production; the method is simple and convenient; the regeneration period is short; the propagation coefficient is high; the transplanting survival rate is high; the seedling germination is regular and consistent; the maintenance and management are convenient; the laborand land resources are saved; the production cost is low; the season limitation is avoided; the regeneration plants are continuously obtained; the method can be used for seedling industrialized production.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation method for inducing plant regeneration by stem segments of Sequoia chinensis. Background technique [0002] Sequoia (scientific name: Sequoia sempervirens) is an evergreen tree of the genus Sequoia in the fir family, native to the coast of California, USA. Referred to as redwood, also known as "World God", it is a single-species plant of the fir family, and it is also a rare relic tree species in the world. It is a "living fossil" in the plant world. At present, this tree species has been widely introduced all over the world. In my country, as early as 1972, when the former US President Nixon visited China, he presented North American Sequoia to China and propagated it in Hangzhou Botanical Garden. At present, it has been introduced and cultivated in Shanghai, Nanjing, Hangzhou and other places. [0003] This tree species has the advantages of fast growth, long duration of fast-growing,...

AI Technical Summary

Problems solved by technology

However, there is a lack of seeds in seed propagation, and the germination rate is low, only 4.5%-11.9%, and the genetic variation of seedlings is large; the rooting situation is unstable in cutting propagation, and the rooting rate can be as low as 16% due to different ear-picking mother plants, and the highest can be up to 100%, but generally lower than 50%, and the plant type of cutting seedlings is not good

As a result, North American Sequoia has been successfully introduced, but its provenance is still relatively small, and the provenance issue has always attracted much attention.

The above studies were carried out for the purpose of obtaining the propagation system, and there was no systematic research on the stability of genetic traits and the robustness of tissue culture seedlings, which have different degrees of influence on the rooting of tissue culture seedlings and transplanting and hardening of seedlings in the later stage.

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