Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Building method for yellow ligament ossification animal model

A technology for ossification of ligamentum flavum and animal model, which is applied in the field of disease animal model construction, can solve problems such as inconsistency in pathogenesis, reduction in specimen volume, spinal cord injury, etc., and achieves increased contact area, prolonged action time, and expanded contact area. Effect

Inactive Publication Date: 2015-09-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main defects of this method are: 1. Too much damage to the ligamentum flavum structure, and the ossification process of the ligamentum flavum is inconsistent with the pathogenesis of human ossification of the ligamentum flavum; 2. Most of the ligamentum flavum in rabbits has been destroyed, and the amount of specimens is greatly reduced , a positive result is difficult to obtain; 3. The structure of the ligamentum flavum in rabbits is small, and ossification-inducing drugs are embedded in the ligamentum flavum or even in the spinal canal, which can easily cause spinal cord damage and are difficult to operate; 4. The combination of drugs and ligamentum flavum The contact area is relatively limited; 5. The carrier is mainly collagen and gelatin sponge, and the intervention drug is easy to lose
[0006] The above defects lead to low success rate of animal models of ossification of ligamentum flavum, and overcoming or improving the above defects is the key to improving the success rate of animal models of ossification of ligamentum flavum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Building method for yellow ligament ossification animal model
  • Building method for yellow ligament ossification animal model
  • Building method for yellow ligament ossification animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Osteopontin+matrigel-induced ossification of the ligamentum flavum in New Zealand white rabbits

[0025] 1. Osteopontin mixed with matrigel

[0026] Characteristics of Matrigel: The main components of Matrigel (purchased from BD Company) are laminin and type IV collagen, which exist in liquid form when the temperature is 0-4°C, and are jelly-like when the temperature is higher than 4°C.

[0027] The method of mixing Matrigel with human osteopontin (purchased from Protech Company): This experimental operation needs to be performed on an ice bath. According to the needs of the experiment, a certain amount of matrigel was mixed with the drug to a drug concentration of 20 ng / μl, and the mixture was fully blown and mixed to avoid the appearance of air bubbles. Then put the drug-containing matrigel in a 4°C refrigerator for storage.

[0028] 2. Construction of OLF animal model of New Zealand white rabbit

[0029] 1) Get a healthy adult New Zealand white rabbit w...

Embodiment 2

[0040] Example 2. Recombinant human bone morphogenetic protein 2+matrigel-induced ossification of the ligamentum flavum in New Zealand white rabbits

[0041] The concentration of recombinant human bone morphogenetic protein 2 (purchased from Shanghai Ruiqi Industrial Technology Co., Ltd.) was 2.5 μg / μl. The rest of the model construction methods were the same as in Example 1. A total of 3 animal models were constructed, all of which were successful.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of a building method for a disease animal model, and provides a building method for a yellow ligament ossification animal model. The building method does not damage the completeness of the yellow ligament. Medicine and the matrigel phase are mixed and then injected to the yellow ligament in a multi-point mode. The time of interaction of the medicine and the yellow ligament is prolonged to the maximum extent. The contact area of the medicine and the yellow ligament is expanded. The animal model is closer to the yellow ligament ossification generation mechanism in the human body, so that damage to the completeness of the yellow ligament is reduced. The success rate of building the model is increased. A certain theoretical basis is provided for the research of the yellow ligament ossification generation mechanism in the human body in future. Thus, the building method for the yellow ligament ossification animal model can be better applied clinically.

Description

Technical field: [0001] The invention relates to the technical field of a method for constructing an animal model of a disease, in particular to a method for constructing an animal model of ossification of the ligamentum flavum. Background technique: [0002] Ossification of ligamentum flavum (OLF) is a heterotopic ossification of the ligamentum flavum in the human spine. This lesion can cause compression on the back of the spinal cord, resulting in quadriplegia and urinary and bowel dysfunction. The pathogenesis of OLF is still unclear. The current experimental research on the pathogenesis of ossification of ligamentum flavum mainly focuses on human ligamentum flavum cells, and animal models are an important means to verify the results of in vitro cytology experiments and disease mechanisms. There is less research. [0003] The occurrence of ossification of ligamentum flavum is a multifactorial pathogenic process. According to the existing literature reports, animal models...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K47/42
Inventor 陈雄生周盛源刘晓东
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com