Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation

A technology of bagasse and succinic acid is applied in the field of bioengineering, which can solve the problems of increased cost and low yield, and achieve the effects of increasing concentration, protecting the environment, and requiring low fermentation equipment.

Inactive Publication Date: 2015-09-09
SHANDONG XINGQIANG CHEM IND TECH RES INST CO LTD
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of succinic acid fermented by these metho

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation
  • Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation
  • Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1: Pretreatment of bagasse with dilute alkali

[0035] Take 20g of the crushed and dried bagasse, mix it with 300mL of 1% NaOH solution, pretreat the bagasse at a high temperature of 121°C for 2h, filter and wash the bagasse to neutrality, and dry at 65°C to obtain 13g of pretreated bagasse. The processed bagasse has a weight loss of 35% and a total cellulose content of 87.6%. Its composition is shown in Table 1.

[0036] Table 1 shows the components of alkaline pretreatment bagasse measured by NREL method

[0037]

Example Embodiment

[0038] Example 2: Multiple enzymes synergistically hydrolyze bagasse treated with alkali

[0039] Weigh 3g of the bagasse pretreated with alkali, transfer it into a 150mL Erlenmeyer flask, add cellulase 25FPU.g -1 , Xylanase 85U.g -1 , Β-glucanase 40U.g -1 , Pectinase 25U.g -1 , Add pH5.0 citric acid buffer, make the volume to 40mL, hydrolyze in a water bath shaker at 50℃ for 40h, and take samples every 4h. Multi-enzyme synergistic hydrolysis time figure 2 . After 40 hours of hydrolysis, the concentration of reducing sugar in the hydrolyzate is 65.6g.L -1 , The total cellulose hydrolysis efficiency of bagasse is up to 90%, and the concentration of glucose in the bagasse hydrolyzate is 45.06g.L -1 , The concentration of xylose is 14.92g.L -1 , The concentration of glucuronic acid is 5.62g.L -1 , The concentration ratio of glucose and xylose is close to 3:1. The time curve of pretreatment of bagasse by multi-enzyme synergistic hydrolysis figure 2 Shown.

Example Embodiment

[0040] Example 3: Production of Succinic Acid by Feeding Fermentation of Bagasse Hydrolysate

[0041] Strain: Actinobacillu ssuccinogenes F3-21. Medium: sugar concentration of bagasse hydrolysate 55g.L -1 , Corn steep liquor concentration 25g.L -1 , Anhydrous calcium chloride concentration 0.2g.L -1 , The concentration of dipotassium hydrogen phosphate 2g.L -1 , The concentration of sodium dihydrogen phosphate 9.6g.L -1 , The concentration of magnesium chloride hexahydrate is 0.2g.L -1 , Sodium sulfide concentration 0.1g.L -1 , PH was adjusted to 6.0, and sterilized at 121°C for 20 minutes.

[0042] On a 3L stirred fermenter, fed-batch fermentation was used, and the initial concentration of the bagasse hydrolyzed sugar solution was 55g.L -1 , When the residual sugar concentration in the fermentation broth drops to 20g.L -1 When using a peristaltic pump, the total reducing sugar concentration is 200g.L -1 Concentrated bagasse hydrolyzed sugar solution, so that the sugar concentration...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation and belongs to the technical field of bioengineering. The method is characterized by comprising the following steps of taking the sugarcane bagasse which is main byproducts in the sugar pressing industry as raw materials, and performing crushing, pretreatment with dilute alkali as well as common hydrolysis on cellulase, xylanase, beta-glucanase and pectinase to obtain a hydrolyzed sugar solution; fermenting the hydrolyzed sugar solution by using actinobacillus succinogenes to produce the succinic acid; meanwhile, residues left by hydrolyzing the sugarcane bagasse are added into fermented solution, so that the fermentation can be repeatedly operated, seed culture can be omitted and furfural in residual liquid obtained by extraction of organic solvent and pretreatment with the dilute alkali can be utilized. According to the method disclosed by the invention, reasonable utilization of hydrolyzed residues of the sugarcane bagasse is realized, and the conventional fermented seed culture link is reduced, so that the fermentation cost is reduced, and the fermentation efficiency is improved; besides, the requirement on fermentation equipment is low, extra improvement on an existing fermentation tank is not needed, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for producing succinic acid by fermenting bagasse as a raw material, and belongs to the technical field of bioengineering. Background technique [0002] Bagasse is an important renewable raw material of lignocellulosic fiber. my country is the country with the third largest planting volume of sugarcane in the world. The south is the main area where sugarcane is planted in China. Tons of bagasse. [0003] The main components in bagasse are cellulose, hemicellulose and lignin. At present, the main uses of bagasse are burning power generation (Light Industry Science and Technology, 2013, (11): 11-12.), as livestock feed (Chinese Patent Application No. 94113455.5), replacing traditional wood resources as papermaking and producing high-density materials ( China Plastics, 2008, (02): 57-61), as a medium for edible fungi (Edible Fungi, 1980, (02): 12-16), and the development and production of high value-added products: such ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/46C07D307/50C12R1/01
CPCC12P7/46
Inventor 郑璞淘声涛查鑫华
Owner SHANDONG XINGQIANG CHEM IND TECH RES INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap