Molecular marker method for rapidly identifying suitability of tea germplasms for manufacture of oolong

A technology of molecular markers and oolong tea, which is applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of long identification time, labor-consuming, low identification efficiency, etc., to shorten the identification cycle, reduce manpower, and reduce blindness sexual effect

Active Publication Date: 2015-09-09
TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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  • Application Information

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Problems solved by technology

The direct identification method has the disadvantages of long identification time, large consum

Method used

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  • Molecular marker method for rapidly identifying suitability of tea germplasms for manufacture of oolong
  • Molecular marker method for rapidly identifying suitability of tea germplasms for manufacture of oolong
  • Molecular marker method for rapidly identifying suitability of tea germplasms for manufacture of oolong

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Determination of the new tea tree strain Chuntaoxiang (strain number 115)

[0059] Chuntaoxiang is a new strain of tea tree clones bred by the Tea Research Institute of Fujian Academy of Agricultural Sciences by hybrid breeding method. Both the strain comparison test and the regional adaptability test show that this strain is suitable for oolong tea ([1] Guo Jichun, et al. Comparative identification of oolong tea strains and selection. Tea Science Briefing, 1994, (145) 4:13-21. [2] Guo Jichun, et al. Preliminary report on regional trials of new varieties of oolong tea. Tea Science and Technology, 1995,149(4):15-20).

[0060] According to the above steps (1) to (4), the genotypes of the new tea tree line Chuntaoxiang at the TM352 and TM445 loci were respectively obtained ( image 3 , Ⅰ, Ⅱ), and then compare it with the genotype of the tea tree germplasm suitable for making oolong tea at the TM352 and TM445 sites, and the genotype is compared with the tea tre...

Embodiment 2

[0061] Example 2, Determination of new tea strain Mingke No. 3 (strain number 511)

[0062] Minke No. 3 is a new strain of tea tree clone bred by the Tea Research Institute of Fujian Academy of Agricultural Sciences by hybrid breeding method. Regional adaptability tests show that this strain is suitable for red and green tea, but not suitable for oolong tea (Zhou Fuyu. Tea tree hybrid Mingke 3 No. National Regional Test Guizhou Point Test Report. Fujian Tea, 2009, 4:5-7).

[0063] According to the above steps (1) to (4), the genotypes of the new tea tree line Mingke No. 3 at the TM352 and TM445 loci were respectively obtained ( Figure 4 , Ⅰ, Ⅱ), and then compare it with the genotypes at the TM352 and TM445 sites of the tea tree germplasm suitable for making oolong tea, although the genotype at the TM445 site of the germplasm ( Figure 4 , Ⅱ) and the genotype of the tea germplasm suitable for oolong tea at the TM445 locus ( figure 2 , a) Consistent, but the genotype of th...

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Abstract

The invention provides a molecular marker method for rapidly identifying suitability of tea germplasms for manufacture of oolong. The method comprises steps as follows: (1) extracting genome DNA of to-be-identified tea germplasms; (2) synthesizing a specific SSR fluorescence-labeled primer; (3) taking the genome DNA of the to-be-identified tea germplasms as a template and performing PCR (polymerase chain reaction) amplification with the specific SSR fluorescence-labeled primer; (4) detecting a PCR amplification product and acquiring genotypes at specific sites of the to-be-identified tea germplasms; (5) analyzing the genotypes of the to-be-identified tea germplasms. The tea germplasms suitable for manufacture of the oolong can be screened out through simple experimental operation and analysis, and only 1-2 workdays are spent on the whole process; the method is used for molecular marker-assisted selection in oolong varieties, directive breeding of oolong varieties can be realized to a certain extent, and the blindness of new oolong variety selection is reduced; genetic backgrounds and relationships among multiple to-be-identified tea germplasms can be mastered preliminarily with the method.

Description

technical field [0001] The invention belongs to the field of tea tree germplasm identification utilization and variety selection. Specifically, it relates to a molecular marker method for quickly identifying the suitability of tea tree germplasm oolong tea. Background technique [0002] Simple sequence repeat (SSR) has the characteristics of high allelic variation, co-dominance, simplicity, speed, and stability. Currently, SSR is one of the main molecular markers for tea germplasm identification, genetic diversity, and kinship research. one. Download the tea tree EST sequence from the NCBI public database, use the software to obtain the microsatellite (SSR) information of the EST sequence, and use the software to design the conserved sequence flanking the microsatellite as primers, and obtain 30 pairs of effective SSR primers through preliminary screening. 328 pairs of tea tree SSR primers (Jian-Qiang Ma, Ming-Zhe Yao, Chun-Lei Ma, etc. Construction of a SSR-Based Genetic ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2525/151
Inventor 王让剑高香凤杨军孔祥瑞郭吉春
Owner TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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