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Growth-promoting solution and method for improving photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets

A technology of growth-promoting liquid and test-tube seedlings, applied in gardening methods, plant growth regulators, plant growth regulators, etc., can solve problems such as high cost, complicated production process, and unsatisfactory growth regulators, and achieve quality improvement and low cost. Inexpensive, good for mass production and application

Active Publication Date: 2017-11-21
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of unsatisfactory traditional sugarcane test-tube plantlet growth regulators, complex production process, strict cultivation environment requirements, and high cost, and provide a growth-promoting liquid formula and method for improving the photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets

Method used

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  • Growth-promoting solution and method for improving photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets
  • Growth-promoting solution and method for improving photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets

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Embodiment 1

[0030] A growth-promoting liquid formula for improving the photoautotrophic rooting efficiency of sugarcane test-tube plantlets. The formula is ABT2 No. rooting powder 100 mg / L + proline 56 mg / L + Tween-80 2.5 ml / L.

[0031] A corresponding method for improving the photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets comprises the following steps:

[0032] (1) Seedling hardening: choose Guitang No. 44 as the sugarcane variety, and the subculture culture cycle will be completed, and the rootless sugarcane seedling bottle containing the subculture medium should be placed in a cool, sheltered place, open the bottle cap, and spray growth promoting Solution 1: Indolebutyric acid 200 mg / L + 6-benzylaminoadenine 5 mg / L + L-proline 56 mg / L + Tween-80 2.5 ml / L, at a temperature of 25°C and a humidity of 80% , under the light intensity of 5000 lux, the seedlings were hardened for 24 hours;

[0033] (2) Divide the test-tube plantlets: take out the test-tube pla...

Embodiment 2

[0040] A growth-promoting liquid formula for improving the photoautotrophic rooting efficiency of sugarcane test-tube plantlets. The formula is ABT2 No. rooting powder 200 mg / L + proline 56 mg / L + Tween-80 2.5 ml / L.

[0041] The corresponding method for improving the photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets comprises the following steps:

[0042] (1) Seedling hardening: choose Guitang No. 44 as the sugarcane variety, and the subculture culture cycle will be completed, and the rootless sugarcane seedling bottle containing the subculture medium should be placed in a cool, sheltered place, open the bottle cap, and spray growth promoting Solution 2: Naphthaleneacetic acid 5 mg / L + L-proline 230 mg / L + Tween-80 2.5 ml / L, harden seedlings for 48 hours at a temperature of 26°C, a humidity of 80%, and a light intensity of 10,000 lux;

[0043] (2) Divide the test-tube plantlets: take out the test-tube plantlets from the bottle after hardening, divide...

Embodiment 3

[0050] A growth-promoting liquid formula for improving the photoautotrophic rooting efficiency of test-tube plantlets of sugarcane, the formula is ABT2 rooting powder 50 mg / L + proline 56 mg / L + Tween-80 2.5 ml / L.

[0051] The corresponding method for improving the photosynthetic autotrophic rooting efficiency of sugarcane test-tube plantlets comprises the following steps:

[0052] (1) Seedling hardening: choose Guitang No. 44 as the sugarcane variety, and the subculture culture cycle will be completed, and the rootless sugarcane seedling bottle containing the subculture medium should be placed in a cool, sheltered place, open the bottle cap, and spray growth promoting Solution 2: Naphthaleneacetic acid 100 mg / L + L-proline 115 mg / L + Tween-80 2.5 ml / L, harden seedlings for 40 hours at a temperature of 27°C, a humidity of 70%, and a light intensity of 5000 lux;

[0053] (2) Divide the test-tube plantlets: take out the test-tube plantlets from the bottle after hardening, divide...

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Abstract

The invention relates to a growth-promoting liquid formula and a method for improving the photoautotrophic rooting efficiency of sugarcane test-tube seedlings. The growth-promoting liquid formula is: ABT2 No. rooting powder 5-300 mg / L + proline 28-230 mg / L + Tween-80 2~2.5 ml / L, this method is to open the bottle of rootless sugarcane seedlings that have completed the subculture multiplication culture cycle to harden the seedlings, spray the growth promoting liquid at the same time, take out the test-tube seedlings after hardening, and divide the test-tube seedlings , disinfection, and finally divide the test-tube seedlings into individual plants or small clusters and plant them in planting pots and put them into the seedling greenhouse for cultivation and rooting. The growth-promoting liquid formula of the present invention has a good penetrating effect, can significantly improve the survival rate of sugarcane test-tube plantlets, delay leaf senescence and enhance the ability to resist environmental stress; the method of the present invention saves the traditional procedure of in-test-tube rooting of rootless test-tube plantlets, The autotrophic rooting is used to replace the traditional heterotrophic rooting, which shortens the seedling cultivation cycle, saves production costs, and is beneficial to the large-scale production and popularization of sugarcane healthy test-tube seedlings.

Description

technical field [0001] The invention relates to a photoautotrophic rooting method for plant test-tube seedlings, in particular to a growth-promoting liquid formula and a method for improving the photosynthetic autotrophic rooting efficiency of sugarcane test-tube seedlings. Background technique [0002] Plant tissue culture generally goes through four stages, namely primary culture (or establishment of a sterile culture system), subculture proliferation culture, rooting culture and seedling hardening and transplanting. At present, the sugarcane test-tube seedling rapid breeding technology that has been applied on a large scale in production also includes the above four stages. However, this traditional sugarcane test-tube plantlet production process is complex and requires high environmental conditions for cultivation, resulting in high cost of sugarcane tissue culture industrial production of test-tube plantlets, which has been unacceptable by the market and has limited the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/36A01P21/00A01G7/06A01N43/38A01N37/10
Inventor 刘红坚何为中
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI