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A kind of preparation method and application of dendrobium candidum stem cell freeze-dried powder

A technology of Dendrobium officinale and stem cells, applied in the field of stem cells, can solve the problems of long cell culture cycle and slow cell proliferation, and achieve the effects of short cell culture cycle, anti-aging cell lifespan, and activation of dormant cells

Active Publication Date: 2018-01-26
GUANGDONG CARECODE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects of long cell culture cycle and slow cell proliferation speed in the prior art Dendrobium candidum plant cell culture technology, the purpose of the present invention is to provide a preparation method and application of Dendrobium candidum stem cell freeze-dried powder to solve the above defects

Method used

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  • A kind of preparation method and application of dendrobium candidum stem cell freeze-dried powder
  • A kind of preparation method and application of dendrobium candidum stem cell freeze-dried powder
  • A kind of preparation method and application of dendrobium candidum stem cell freeze-dried powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the preparation of dendrobium officinale stem cell freeze-dried powder

[0041] S1. Take the root tip of Dendrobium officinale with the root cap removed, wash it with water for 2 hours, then disinfect it with 70% alcohol for 10 seconds, rinse it with sterile water for 3 times, then disinfect it with 0.1% tribute solution for 10 minutes, and rinse it with sterile water for 3 times ,spare;

[0042]S2. Cut the root tip of Dendrobium officinale sterilized in step S1 into 5mm*5mm size, 1 piece per bottle, inoculate to contain 2mg / L zinc sulfate, 77 mg / L citric acid, 79mg / L glycine, 2mg / L α- On the solid medium of naphthaleneacetic acid, 10.5mg / L sucrose, 460 mg / L gac and 2.8 mg / L agar, culture temperature is 25 ℃, pH value is 6, culture 20 days, obtain the dendrobium candidum stem cell of cambium origin;

[0043] S3. Transfer the stem cells of Dendrobium officinale derived from the cambium to a culture medium containing 181 mg / L magnesium sulfate, 77 mg / L citr...

Embodiment 2

[0047] Embodiment 2, the preparation of dendrobium officinale stem cell freeze-dried powder

[0048] S1. Take the root tip of Dendrobium officinale with the root cap removed, wash it with water for 3 hours, then disinfect it with 75% alcohol for 15 seconds, rinse it with sterile water for 4 times, and then disinfect it with 0.1% Ligong solution for 10 minutes, and rinse it with sterile water for 4 times ,spare;

[0049] S2. Cut the root tip of Dendrobium officinale sterilized in step S1 into 5mm*5mm size, 1 piece per bottle, inoculate to contain 2.5mg / L zinc sulfate, 80mg / L citric acid, 82mg / L glycine, 2.5mg / L α - on the solid medium of naphthaleneacetic acid, 11mg / L sucrose, 500mg / L activated carbon and 3mg / L agar, culture temperature is 27 ℃, pH value is 6.5, culture 25 days, obtain the dendrobium officinale stem cell of cambium origin;

[0050] S3. The stem cells of Dendrobium candidum derived from the cambium were transferred to a mixture containing 182mg / L magnesium sulf...

Embodiment 3

[0054] Embodiment 3, the preparation of dendrobium officinale stem cell freeze-dried powder

[0055] S1. Take the root tip of Dendrobium officinale with the root cap removed, wash it with water for 4 hours, then disinfect it with 75% alcohol for 10 seconds, rinse it with sterile water for 5 times, then disinfect it with 0.1% Ligong solution for 10 minutes, and rinse it with sterile water for 5 times ,spare;

[0056] S2. Cut the root tip of Dendrobium officinale sterilized in step S1 into 5mm*5mm size, 1 piece per bottle, inoculate to contain 3mg / L zinc sulfate, 83mg / L citric acid, 84mg / L glycine, 2.8mg / L α- On the solid medium of naphthaleneacetic acid, 11.5mg / L sucrose, 540mg / L gac and 3.5mg / L agar, culture temperature is 29 ℃, pH value is 6.5, culture 30 days, obtain the dendrobium candidum stem cell of cambium origin;

[0057] S3. The stem cells of Dendrobium officinale derived from the cambium were transferred to granules containing 183mg / L magnesium sulfate, 82mg / L citri...

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Abstract

The invention belongs to the field of stem cells, and particularly relates to a preparation method of dendrobium officinale stem cell freeze-dried powder and application thereof. The preparation method comprises the following steps: after cleaning and sterilizing root tips of dendrobium officinale with removed root caps, separating stem cells of dendrobium officinale by utilizing a solid culture medium, and performing serial subcultivation by utilizing a liquid culture medium, after filtering, performing freeze-drying to prepare the dendrobium officinale stem cell freeze-dried powder. By experimental evidence, the dendrobium officinale stem cell freeze-dried powder prepared by adopting the preparation method can effectively improve the activity of SOD enzyme in cells, can reduce the content of MDA, attack and damage of oxygen radicals produced by ultraviolet radiation and induction to the cells and the death rate of cells, can maintain the internal antioxidant balance system, so as to play the role of protecting cells and achieve an anti-aging effect.

Description

technical field [0001] The invention belongs to the field of stem cells, and in particular relates to a preparation method and application of dendrobium officinale stem cell freeze-dried powder. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells, known as "universal cells". Aging is a complex biological process, which is not only affected by gene regulation, disease, bad living habits and social and psychological load, but also affected by environmental factors. At present, environmental factors that have adverse effects on the skin mainly include ultraviolet and infrared rays in sunlight, cigarette smoke and air pollution, among which ultraviolet rays have a particularly significant impact, which can accelerate the process of physiological aging. In recent years, Swiss scientists have discovered that an apple stem cell has the functions of...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01N3/00A61K8/9794A61Q19/00A61Q19/08
Inventor 王一飞廖晓凤卓翠琴
Owner GUANGDONG CARECODE BIOLOGICAL TECH CO LTD