Method for detecting total bacteria count in milk

A technology for the total number of bacteria and detection methods, applied in the field of food analysis, can solve the problems of difficult monitoring and control, time-consuming and laborious, secondary pollution, etc., and achieve the effects of short detection time, accurate detection results and high correlation

Inactive Publication Date: 2015-09-23
DONGCHEN LEADER TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These standard detection methods are based on plate culture and colony counting, which takes about 48 hours. For manufacturers to purchase fresh milk and evaluate and grade, the detection process is time-consuming and laborious, wasting a lot of manpower, material and financial resources, and greatly im

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The detection method of the total number of bacteria in milk comprises the following steps:

[0021] Step 1, pour the milk into the sterile saline, shake at 100prm for 2min, and get the mixture;

[0022] Step 2, add xylene to the mixed solution obtained in step 1, stir, centrifuge at 1000rpm for 15min, and remove the upper layer;

[0023] Step 3, add a mixed enzyme solution containing trypsin, papain and chymotrypsin to the lower layer retention solution obtained in step 2, and bathe in water at 40°C for 5 minutes;

[0024] Step 4, concentrating the enzymolysis solution obtained in step 3 with a nanofiltration membrane;

[0025] Step 5, take the concentrated solution obtained in step 4, and after staining with methylene blue staining solution, detect and count with a microbiological detector.

[0026] The volume ratio of milk and sterile saline in step 1 is 5:50.

[0027] The volume ratio of the mixed liquid and xylene in step 2 is 5:2.

[0028] The volume ratio of ...

Embodiment 2

[0033] The detection method of the total number of bacteria in milk comprises the following steps:

[0034] Step 1: Pour milk into sterile saline, shake at 110prm for 2min, and obtain a mixture;

[0035] Step 2, add xylene to the mixed solution obtained in step 1, stir, centrifuge at 1050rpm for 10min, and remove the upper layer;

[0036] Step 3, add a mixed enzyme solution containing trypsin, papain and chymotrypsin to the lower layer retention solution obtained in step 2, and bathe in water at 40°C for 5 minutes;

[0037] Step 4, concentrating the enzymolysis solution obtained in step 3 with a nanofiltration membrane;

[0038] Step 5, take the concentrated solution obtained in step 4, and after staining with methylene blue staining solution, detect and count with a microbiological detector.

[0039] The volume ratio of milk and sterile saline in step 1 is 9:75.

[0040] The volume ratio of the mixed liquid and xylene in step 2 is 10:6.

[0041] The volume ratio of the lo...

Embodiment 3

[0046] The detection method of the total number of bacteria in milk comprises the following steps:

[0047] Step 1, pour the milk into the sterile saline, shake at 140prm for 2min, and get the mixture;

[0048] Step 2, add xylene to the mixed solution obtained in step 1, stir, centrifuge at 1250rpm for 8min, and remove the upper layer;

[0049] Step 3, add a mixed enzyme solution containing trypsin, papain and chymotrypsin to the lower layer retention solution obtained in step 2, and bathe in water at 40°C for 5 minutes;

[0050] Step 4, concentrating the enzymolysis solution obtained in step 3 with a nanofiltration membrane;

[0051] Step 5, take the concentrated solution obtained in step 4, and after staining with methylene blue staining solution, detect and count with a microbiological detector.

[0052] The volume ratio of milk and sterile saline in step 1 is 14:95.

[0053] The volume ratio of the mixed liquid and xylene in step 2 is 13:6.

[0054] The volume ratio of...

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Abstract

The invention discloses a method for detecting total bacteria count in milk. The method includes pouring milk into sterile saline solution, oscillating at 100-150 rpm for 2 min to obtain mixed solution, adding xylene into the mixed solution, stirring and centrifuging at 1000-1500 rpm for 5-15 min, and removing the upper layer; adding mixed enzyme solution containing trypsin, papain and chymotrypsin into the residual liquid, water-bathing 5 min at 40 degrees Centigrade to obtain enzymatic hydrolysate, concentrating by a nanofiltration membrane, and dyeing the concentrate by methylene blue dyeing liquid and detecting and counting by a microorganism detector. The method for detecting total bacteria count in milk has no remarkable difference as compared with an international method and is high in correlation, accurate in detecting results and short in detecting time and can be applied to actual detection, and detection limit is 20-106 cfu/mL.

Description

technical field [0001] The invention belongs to the technical field of food analysis, and in particular relates to a method for detecting the total number of bacteria in milk products. Background technique [0002] Milk is one of the oldest natural beverages, and it is also an indispensable food with high nutritional value in modern human life. At present, my country's dairy industry is developing rapidly. In 2012, my country's consumption of liquid dairy products reached about 22 million tons. Dairy production technology and equipment are updated rapidly, and market competition is fierce. Compared with developed countries, my country has a big gap in terms of scale, technology, and product quality. The entry of foreign brands into the Chinese market has caused a great impact on local industries. The root cause of the gap in product quality is the uneven quality of raw milk. At present, 80% of our country is milked by hand. The free-range breeding of dairy cows and self-emp...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 熊开胜张海防谢建庭
Owner DONGCHEN LEADER TESTING
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