Method for super-sensitively detecting alpha fetalprotein based on surface enhanced raman scattering effect of silver nanoparticle tripolymer

A surface-enhanced Raman and silver nanoparticle technology, applied in the field of analytical chemistry, can solve the problems of affecting the results, low sensitivity of the ELISA method, and insufficient tissue sample processing, and achieve the effect of stabilizing the detection limit and improving the sensitivity.

Active Publication Date: 2015-09-23
JIANGNAN UNIV
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Problems solved by technology

At present, the methods for detecting AFP mainly include radioimmunoassay and enzyme-linked immunoassay (ELISA), but both have certain advantages and disadvantages, and the scope of application is also different, so the clinical application of these two methods needs to be considered. Inadequacies
When detecting tumor markers by ELISA, it is difficult to detect pro

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  • Method for super-sensitively detecting alpha fetalprotein based on surface enhanced raman scattering effect of silver nanoparticle tripolymer
  • Method for super-sensitively detecting alpha fetalprotein based on surface enhanced raman scattering effect of silver nanoparticle tripolymer
  • Method for super-sensitively detecting alpha fetalprotein based on surface enhanced raman scattering effect of silver nanoparticle tripolymer

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Embodiment 1

[0022] Example 1 Method for ultrasensitive detection of alpha-fetoprotein based on surface-enhanced Raman scattering effect of silver nanoparticle trimer

[0023] (1) Synthesis of silver nanoparticle trimer: Synthesis of 15 nm silver nanoparticles was carried out according to the content disclosed in the application number 201110274220.X; 20 mL of ultrapure water was mixed with 5 mL of polyvinylpyrrolidone with a mass fraction of 1%, and then Add 0.6 mL of 0.1 mol / L sodium borohydride aqueous solution, and carry out the reaction in an ice bath. Then, 5 mL of polyvinylpyrrolidone aqueous solution with a mass fraction of 1% and 5 mL of silver nitrate aqueous solution with a mass fraction of 0.15% were added to the above solution at the same time at a rate of 30 mL / h with a syringe pump, paying attention to adding while stirring. Immediately after the addition, it was placed in a water bath at 80° C. for 2 hours to obtain 15 nm silver nanoparticles.

[0024] Centrifuge 100 mL of...

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Abstract

The invention provides a method for super-sensitively detecting alpha fetalprotein based on the surface enhanced raman scattering effect of a silver nanoparticle tripolymer and belongs to the technical field of analytical chemistry. The method comprises the following steps: synthetizing silver nanoparticles with the grain sizes of 15 nm, modifying DNA by the silver nanoparticles, assembling the silver nanoparticle tripolymer, and conducting TEM characterizing and raman signal testing on the silver nanoparticle tripolymer. The silver nanoparticle tripolymer is assembled for the first time, and has a strong raman signal, the raman signal changes after the alpha fetoprotein is added, and the quick super-sensitive detection of the alpha fetalprotein is achieved by the change of the raman signal.

Description

technical field [0001] The invention relates to a method for ultrasensitive detection of alpha-fetoprotein based on the surface-enhanced Raman scattering effect of silver nanoparticle trimer, and belongs to the technical field of analytical chemistry. Background technique [0002] Alpha-fetoprotein (α-fetoprotein, AFP), a carcinogenic glycoprotein, serves as a "molecular marker" of adult physiological status, and is often used as a suitable marker for the diagnosis of hepatocellular carcinoma, yolk sac tumor, germ cell tumor, and gastric cancer. of biomarkers. At present, the methods for detecting AFP mainly include radioimmunoassay and enzyme-linked immunoassay (ELISA), but both have certain advantages and disadvantages, and the scope of application is also different, so the clinical application of these two methods needs to be considered. Inadequacies. When detecting tumor markers by ELISA, it is difficult to detect protein tumor markers because proteins are easily therm...

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Application Information

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IPC IPC(8): G01N21/65
Inventor 匡华付盼胥传来徐丽广马伟刘丽强宋珊珊胡拥明
Owner JIANGNAN UNIV
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