Method for super-sensitively detecting alpha fetalprotein based on surface enhanced raman scattering effect of silver nanoparticle tripolymer

Abstract

The invention provides a method for super-sensitively detecting alpha fetalprotein based on the surface enhanced raman scattering effect of a silver nanoparticle tripolymer and belongs to the technical field of analytical chemistry. The method comprises the following steps: synthetizing silver nanoparticles with the grain sizes of 15 nm, modifying DNA by the silver nanoparticles, assembling the silver nanoparticle tripolymer, and conducting TEM characterizing and raman signal testing on the silver nanoparticle tripolymer. The silver nanoparticle tripolymer is assembled for the first time, and has a strong raman signal, the raman signal changes after the alpha fetoprotein is added, and the quick super-sensitive detection of the alpha fetalprotein is achieved by the change of the raman signal.

Description

technical field [0001] The invention relates to a method for ultrasensitive detection of alpha-fetoprotein based on the surface-enhanced Raman scattering effect of silver nanoparticle trimer, and belongs to the technical field of analytical chemistry. Background technique [0002] Alpha-fetoprotein (α-fetoprotein, AFP), a carcinogenic glycoprotein, serves as a "molecular marker" of adult physiological status, and is often used as a suitable marker for the diagnosis of hepatocellular carcinoma, yolk sac tumor, germ cell tumor, and gastric cancer. of biomarkers. At present, the methods for detecting AFP mainly include radioimmunoassay and enzyme-linked immunoassay (ELISA), but both have certain advantages and disadvantages, and the scope of application is also different, so the clinical application of these two methods needs to be considered. Inadequacies. When detecting tumor markers by ELISA, it is difficult to detect protein tumor markers because proteins are easily therm...

AI Technical Summary

Problems solved by technology

At present, the methods for detecting AFP mainly include radioimmunoassay and enzyme-linked immunoassay (ELISA), but both have certain advantages and disadvantages, and the scope of application is also different, so the clinical application of these two methods needs to be considered. Inadequacies

When detecting tumor markers by ELISA, it is difficult to detect protein tumor markers because proteins are easily thermally denatured. In addition, the sensitivity of ELISA is low, and it is labor-intensive and time-consuming; radioimmunoassay sometimes has cross-reactions and false positives Reaction, tissue sample processing is not fast enough, cannot inactivate degradative enzymes, salt and pH sometimes affect the results, etc.

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