A method for ultrasensitive detection of alpha-fetoprotein based on surface-enhanced Raman scattering effect of silver nanoparticle trimers

Abstract

The invention discloses a method for ultrasensitive detection of alpha-fetoprotein based on the surface-enhanced Raman scattering effect of silver nanoparticle trimer, and belongs to the technical field of analytical chemistry. The invention specifically includes the synthesis of silver nanoparticles with a diameter of 15nm, the assembly of silver nanoparticles modified DNA, and the trimer of silver nanoparticles, and performing TEM characterization and Raman signal testing on them. The present invention assembles silver nanoparticle trimers for the first time. The silver nanoparticle trimers have a strong Raman signal. Rapid ultrasensitive detection.

Description

technical field [0001] The invention relates to a method for ultrasensitive detection of alpha-fetoprotein based on the surface-enhanced Raman scattering effect of silver nanoparticle trimer, and belongs to the technical field of analytical chemistry. Background technique [0002] Alpha-fetoprotein (α-fetoprotein, AFP), a carcinogenic glycoprotein, serves as a "molecular marker" of adult physiological status, and is often used as a suitable marker for the diagnosis of hepatocellular carcinoma, yolk sac tumor, germ cell tumor, and gastric cancer. of biomarkers. At present, the methods for detecting AFP mainly include radioimmunoassay and enzyme-linked immunoassay (ELISA), but both have certain advantages and disadvantages, and the scope of application is also different, so the clinical application of these two methods needs to be considered. Inadequacies. When detecting tumor markers by ELISA, it is difficult to detect protein tumor markers because proteins are easily therm...

AI Technical Summary

Problems solved by technology

At present, the methods for detecting AFP mainly include radioimmunoassay and enzyme-linked immunoassay (ELISA), but both have certain advantages and disadvantages, and the scope of application is also different, so the clinical application of these two methods needs to be considered. Inadequacies

When detecting tumor markers by ELISA, it is difficult to detect protein tumor markers because proteins are easily thermally denatured. In addition, the sensitivity of ELISA is low, and it is labor-intensive and time-consuming; radioimmunoassay sometimes has cross-reactions and false positives Reaction, tissue sample processing is not fast enough, cannot inactivate degradative enzymes, salt and pH sometimes affect the results, etc.

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