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Method for eliciting an immune response to an immunogen

A technology for immunogens and uses, applied in the field of eliciting immune responses to immunogens, which can solve problems such as expensive, unrecognizable target proteins, and lengthy

Active Publication Date: 2020-02-07
AGENCY FOR SCI TECH & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is a high risk that antibodies generated from the isolated peptide may not recognize the target protein in vivo after all
[0007] While transfected cells and lipid-based systems have been used to present membrane protein antigens to increase the chances of isolating antibodies that may be effective in vivo, these systems are often unstable, lengthy and expensive

Method used

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  • Method for eliciting an immune response to an immunogen
  • Method for eliciting an immune response to an immunogen
  • Method for eliciting an immune response to an immunogen

Examples

Experimental program
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Embodiment 1

[0058] A method for using alpha-hemolysin to present polymer vesicles to elicit an immune response to alpha-hemolysin in mice is now described in the following paragraphs.

[0059] Materials & methods

[0060] Poly(butadiene-b-ethylene oxide) (PBd 21 -PEO 14 ) BD21 amphiphilic block copolymer was purchased from Polymer Source (Polymer Source, Canada). Alpha-hemolysin comes from Staphylococcus aureus, 3-(N-morpholino)propanesulfonic acid (MOPS), tris(hydroxymethyl)aminomethane hydrochloride (Tris), magnesium chloride and sodium chloride were purchased from Sigma Aldrich Corporation (Singapore). Tetrahydrofuran (THF) was purchased from Tedia (Ohio, USA).

[0061] Preparation of polymer vesicles

[0062] The polymer vesicles are prepared by a membrane rehydration method. The BD21 polymer was dissolved in THF and dried into a thin film on the wall of the conical bottom glass tube under nitrogen flow. The polymer film is further dried under vacuum. Subsequently, ultrapure water was ...

Embodiment 2

[0077] A method of using hemagglutinin to present polymer vesicles to elicit an immune response in mice is now described in the following paragraphs.

[0078] Preparation of hemagglutinin polymer vesicles

[0079] The polymer vesicles were prepared by the membrane rehydration method. BD21 polymer is dissolved in chloroform and dried into a film. Subsequently, ultrapure water was added to rehydrate the membrane and allow the spontaneous formation of polymer vesicles. The resulting vesicle dispersion was then extruded with 0.45 μm and 0.22 μm membranes, and dialyzed against ultrapure water to remove any remaining solvent.

[0080] Recombinant hemagglutinin (HA) protein (influenza A virus H3N2 Wisconsin 67 / 05 , MyBioSource) is provided as a sterile filtered solution in 10 mM sodium phosphate, pH 7.4, 150 mM NaCl and 0.005% Tween-20.

[0081] HA polymer vesicles are formed by adding an aliquot of the HA solution to the polymer vesicle dispersion. The mixture is then incubated to allo...

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Abstract

The present invention relates to methods for eliciting an immune response to an immunogen, and in particular, to such methods using polymersomes as carriers for the immunogen.

Description

[0001] Cross reference of related applications [0002] This application claims the right of priority for Singapore Patent Application No. 201208483-6 filed on November 19, 2012, the content of which is hereby incorporated by reference in its entirety for all purposes. Technical field [0003] The present invention relates to a method for eliciting an immune response to an immunogen, and in particular, to such a method using a polymersome as a carrier of the immunogen. Background technique [0004] Although immunization is a mature process, there are differences in the level of response elicited between different immunogens or antigens (used interchangeably herein). Interestingly, membrane proteins form a class of antigens that produce a low level of response, which in turn means that a large amount of membrane proteins are needed to generate or induce an immune response to the required level. Membrane proteins are notoriously difficult to synthesize and are insoluble in water in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/10
CPCA61K9/0019A61K47/34A61K39/12A61K2039/55566A61K9/1273A61K39/385A61K2039/6093C12N2760/16134A61P37/04
Inventor 马达范·纳拉尼法比安·德卡约傅智康苏杏芳
Owner AGENCY FOR SCI TECH & RES