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A kind of method of dendrobium candidum tissue culture propagation

A Dendrobium officinale and tissue culture technology, applied in the agricultural field, can solve the problems of occupying culture racks, long production cycle, and long single cycle time, and achieve the effects of shortening single cycle and growth cycle, improving survival rate, and great promotion value

Inactive Publication Date: 2017-09-22
GUANGXI JIANBAO SHIHU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The single cycle of production and cultivation is long. In the production of Dendrobium candidum tissue culture, the general single cycle of cultivation (that is, from inoculation to new medium to cultivation) is more than 30 days
[0004] 2. Due to the long production cycle, the turnover of the production site is slow, and a large number of cultivation racks are occupied, resulting in an increase in the area of ​​the plant
[0005] The above shortcomings are common problems in traditional tissue culture production. In the tropical and subtropical regions of southern China, tissue culture seedlings of Dendrobium officinale have become a large variety of seedlings, and there is a market demand of more than hundreds of millions of plants every year. However, due to the production cost The year-by-year increase (labor costs, site rent, etc.) has greatly hindered the development of the seedling industrialization industry. Therefore, a new tissue culture and propagation technology of Dendrobium candidum was developed to overcome the shortcomings of the original culture method and ensure the growth of Dendrobium candidum species. Further development of seedling industry

Method used

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  • A kind of method of dendrobium candidum tissue culture propagation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for tissue culture propagation of dendrobium officinale, comprising the steps of:

[0032] (1) selection and processing of explants:

[0033] Choose the stem of Dendrobium candidum with a diameter of 0.2cm, use alcohol to sterilize the stem of Dendrobium candidum under the aseptic ultra-clean table to carry out crosscutting, then longitudinally cut to obtain long 0.5cm, wide 0.1cm stem slices for use;

[0034] (2) Induced callus culture

[0035] Use tweezers to put the stem slices on the callus culture medium, press the stem slices lightly, so that the stem slices are close to the callus culture medium, and the medium formula for the callus culture is: 1 / 2MS+ NAA (naphthalene acetic acid) 0.1mg / L+KT (kinetin) 0.3mg / L+BA (mitogen) 0.1mg / L+5g / L agar+20g / L sucrose+5g / L peanut juice, pH 5.0, At 25°C, light and dark were alternately cultured for 20 days to obtain callus tissue;

[0036] (3) Differentiation culture

[0037] Transfer the callus to MS+6-BA (6 benzy...

Embodiment 2

[0045] A method for tissue culture propagation of dendrobium officinale, comprising the steps of:

[0046] (1) selection and processing of explants:

[0047] Choose the stem of Dendrobium candidum with a diameter of 0.5cm, use alcohol to sterilize the stem of Dendrobium candidum under the aseptic ultra-clean table to carry out crosscutting, then longitudinally cut to obtain long 2cm, wide 0.3cm stem slices for use;

[0048] (2) Induced callus culture

[0049]Use tweezers to put the stem slices on the callus culture medium, press the stem slices lightly, so that the stem slices are close to the callus culture medium, and the medium formula for the callus culture is: 1 / 2MS+ NAA (naphthalene acetic acid) 0.2mg / L+KT (kinetin) 0.5mg / L+BA (mitogen) 0.3mg / L+8g / L agar+30g / L sucrose+8g / L peanut juice, PH is 5.5, At 30°C, light and dark were alternately cultured for 20 days to obtain callus tissue;

[0050] (3) Differentiation culture

[0051] Transfer the callus to MS+6-BA (6 benzy...

Embodiment 3

[0059] A method for tissue culture propagation of dendrobium officinale, comprising the steps of:

[0060] (1) selection and processing of explants:

[0061] Choose the stem of Dendrobium candidum with a diameter of 0.3cm, use alcohol disinfection to carry out crosscutting to the stem of Dendrobium candidum under the aseptic ultra-clean table, then longitudinally cut to obtain long 0.8cm, wide 0.15cm stem slices for use;

[0062] (2) Induced callus culture

[0063] Use tweezers to put the stem slices on the callus culture medium, press the stem slices lightly, so that the stem slices are close to the callus culture medium, and the medium formula for the callus culture is: 1 / 2MS+ NAA (naphthalene acetic acid) 0.15mg / L+KT (kinetin) 0.35mg / L+BA (mitogen) 0.15mg / L+6g / L agar+25g / L sucrose+6g / L peanut juice, pH 5.0, At 26°C, light and dark were alternately cultured for 20 days to obtain callus tissue;

[0064] (3) Differentiation culture

[0065] Transfer the callus to MS+6-BA (...

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Abstract

The invention discloses a method for tissue culture and propagation of Dendrobium officinale, comprising the following steps: (1) selection and treatment of explants; (2) induced callus culture; (3) differentiation culture; (4) culture of strong seedlings (5) rooting culture; (6) hardening and transplanting. The invention provides a method for tissue culture and propagation of dendrobium candidum, which has the advantages of shortening the single cycle of production and cultivation and the growth cycle, and improving the survival rate of seedlings, thereby reducing investment funds, reducing production costs, and increasing the profit of industrialized production.

Description

technical field [0001] The invention belongs to the field of agricultural technology, in particular to a method for tissue culture and propagation of dendrobium officinale. Background technique [0002] Dendrobium officinale (Musa balbisiana Colla) is one of the important tropical fruits in southern my country, and it is also an important source of economic income in many areas, and many farmers depend on planting Dendrobium officinale to get rid of poverty and become rich. Most of the cultivated edible Dendrobium are unisexual triploid AAA, which has a high degree of sterility and mainly rely on asexual reproduction. In production, sucking buds are usually used for reproduction. When the seedlings are insufficient, cut underground stems of Dendrobium candidum can also be used. Blocks are used to propagate seedlings, but long-term use of this method will accumulate many viruses, such as: bunchy top disease, mosaic disease, etc. cause serious harm. In addition, bad Dendrobi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 何忠海
Owner GUANGXI JIANBAO SHIHU