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Automatic anti-toxin gel cutting machine, imager and gel cutting method of anti-toxin automatic gel cutting machine

A cutter, imager technology, used in metal processing and other directions

Inactive Publication Date: 2015-10-07
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no reports of such devices

Method used

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  • Automatic anti-toxin gel cutting machine, imager and gel cutting method of anti-toxin automatic gel cutting machine
  • Automatic anti-toxin gel cutting machine, imager and gel cutting method of anti-toxin automatic gel cutting machine
  • Automatic anti-toxin gel cutting machine, imager and gel cutting method of anti-toxin automatic gel cutting machine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Please see figure 1 , figure 1 Is the schematic diagram of the chassis structure. The chassis 1 is square, and the chassis 1 is provided with a first grid 11 .

[0041] Put the stained gel on the chassis 1 and put it into the imager together, turn on the ultraviolet light and observe it through the computer, and take photos containing the shape of the gene band and the first grid 11 on the chassis 1 at the same time, and compare the pictures, Artificially use cold tools to cut on the chassis 1 with the grid 11 .

Embodiment 2

[0043] Please see figure 2 , figure 2 It is a schematic diagram of the structure of the cutting needle cover. The cover 2 is provided with a second grid 21, and the node of the second grid 21 is provided with a through hole 22, and a cutting needle 23 is slidingly connected in the through hole 22, and the cutting needle 23 is generally rectangular Arrayed.

[0044] Put the gel on the chassis 1, then put it into the imager for imaging, take a picture including the shape of the gene band and the first grid 11 through the computer, compare the picture, and put the cutting needle at the corresponding node on the cover 2 23 is pushed into the cover 2, and finally the cover 2 is buckled on the chassis 1 to complete tapping.

Embodiment 3

[0046] Please see image 3 , image 3 Schematic diagram of the structure of the cutting disc cover. The cover 2 is provided with a second grid 21, the top inside of the cover 2 is provided with a connection layer (not shown), and the connection layer is provided with a cutting sheet 24, the cutting sheet 24 is strip-shaped, Made of flexible metal material.

[0047] Put the gel on the chassis 1, then put it into the imager for imaging, take a picture including the shape of the gene band and the first grid 11 through the computer, compare the picture, and bend the cutting piece 24 into the shape recorded on the picture , and fixed on the corresponding position on the connection layer, and finally the cover 2 is buckled on the chassis 1 to complete tapping.

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Abstract

The invention relates to an automatic anti-toxin gel cutting machine and an imager. The automatic anti-toxin gel cutting machine comprises a base plate and a cold cutter. The base plate is square and is provided with a first grid. The cold cutter is a cover lid. A second grid is arranged on the top of the cover lid. The first grid is the same with the second grid. Cutting needles or cutting pieces are arranged on the cover lid. The base plate is matched with the cover lid. The anti-toxin gel cutting machine is arranged in the imager. The invention further provides a cutting method of the anti-toxin gel cutting machine. The automatic anti-toxin gel cutting machine, the imager and the cutting method of the anti-toxin automatic gel cutting machine have the advantages that the shape and the position of gel to be cut can be determined through the grid on the base plate, gel cutting is completed in the imager under the coordination of the cover lid, a human body is prevented from making contact with ultraviolet rays and ED colorants during gel cutting, and therefore the harm of the ultraviolet rays and the ED colorants to the human body is avoided.

Description

technical field [0001] The invention relates to a gel cutting device, in particular to an anti-virus automatic gel cutter and imager and a glue tapping method thereof. Background technique [0002] At present, the most commonly used technology in biotechnology belongs to PCR technology. The research on gene molecules must start from here. The most basic verification of PCR products is to run the sample on agarose gel electrophoresis, and perform electrophoresis separation based on the molecular weight of the amplified gene. , forming bands of genes with different molecular weights. The separated gene bands can only be seen after imaging with an imager, and EB staining agent and ultraviolet irradiation are necessary for gel imaging. EB staining agent is a highly sensitive fluorescent staining agent. It is excited by a standard 302nm ultraviolet light transilluminator and emits an orange-red signal. However, EB has certain carcinogenicity and will be volatile. It is easy to p...

Claims

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Application Information

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IPC IPC(8): B26D1/09B26D7/27B26D7/01
Inventor 李家乐彭茂潇牛东红谢淑媚黄文吉王飞王泽卫侃韵杜蕴超
Owner SHANGHAI OCEAN UNIV
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