Pseudomonas maltophilia stain QBA-5 with effect of inhibiting Botrytis cinerea and application thereof

A QBA-5, Pseudomonas technology, applied in the field of microorganisms, can solve the problems affecting the sustainable and healthy development of agriculture, excessive pesticide residues, environmental pollution, etc., and achieve the effect of good market application prospects

Inactive Publication Date: 2015-10-07
QINGDAO AGRI UNIV
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  • Abstract
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Problems solved by technology

[0003] Botrytis cinerea reproduces very fast and produces a large number of conidia, so it is easy to mutate and produce drug resistance. At present, it has been used in agricultural production for benzimazoles, dicarboximides, and carbamates. Resistance to various fungicides such as fungicides, which not only leads to the reduction of control effi...

Method used

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  • Pseudomonas maltophilia stain QBA-5 with effect of inhibiting Botrytis cinerea and application thereof
  • Pseudomonas maltophilia stain QBA-5 with effect of inhibiting Botrytis cinerea and application thereof
  • Pseudomonas maltophilia stain QBA-5 with effect of inhibiting Botrytis cinerea and application thereof

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Embodiment 1

[0020] 1. Isolation and screening of strains

[0021] On March 19, 2013, 20 tomato leaf samples were collected in a greenhouse in Laiyang City. Each leaf was soaked in 75% alcohol for 10 seconds, then soaked in 0.1% mercury chloride for 30 seconds, rinsed with sterile water for 3 times, and transferred to sterilized water. Grind in a mortar treated with bacteria, add sterile water to dilute to 10ml, continue to dilute to 10ml with sterile water -5 times, take 100ul respectively and apply LB plates (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, pH 7.0), and culture at 30°C.

[0022] Pick a single colony and inoculate it in YEPD medium (yeast extract 3 g / L, peptone 10 g / L, glucose 20 g / L) at 25°C and 120 rpm for 24 hours, then culture at 25°C and 12000 rpm Centrifuge under the conditions, filter the supernatant through a filter with a diameter of 0.22um, inoculate the conidia of Botrytis cinerea into the filtrate and let it stand for cultivation, inoculate the co...

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Abstract

The invention discloses a Pseudomonas maltophilia stain QBA-5 with an effect of inhibiting Botrytis cinerea and application thereof. The strain QBA-5 obtained by the invention through screening is named as Pseudomonas maltophilia and is preserved in China Center for Type Culture Collection, with the preservation number CCTCC M 2015403. Specifically through experiments to Botrytis cinerea spores, germ tube elongation and detached leaves, the strain QBA-5 obtained by the invention is proved to have the effect of inhibiting Botrytis cinerea, can be prepared into bio-control agent for preventing and controlling Botrytis cinerea, and has a good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Pseudomonas maltophilia strain QBA-5 capable of inhibiting Botrytis cinerea and its application. Background technique [0002] Botrytis cinerea is an important plant pathogenic fungus widely distributed in the world. There are many kinds of hosts of this fungus, which can infect the stems, leaves, flowers and fruits of 235 kinds of plants, such as tomatoes and cucumbers, and grapes and strawberries. mildew. The disease can occur not only during the growing season of the host plant, but also during the storage of agricultural products, so the damage is serious. Especially in recent years, the large-scale promotion of protected cultivation methods has led to wide sources of infection and rapid spread of the fungus. Botrytis cinerea is becoming more and more serious, and its occurrence and prevalence often cause serious economic losses. According to reports, the occ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
CPCA01N63/10C12N1/205C12R2001/38
Inventor 周善跃高攀梁文星李保华李宝笃
Owner QINGDAO AGRI UNIV
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