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Vibrio diazotrophicus strain for resisting citruscanker

A technology for citrus canker, Vibrio diazotroph, applied in bacteria, microbe-based methods, chemicals for biological control, etc., to solve problems such as shortages

Inactive Publication Date: 2015-10-07
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of effective management of the cultivation of some susceptible varieties, the severity of citrus diseases has gradually increased and it has become an inevitable trend. How to take reasonable and effective measures for management and control in a timely manner is crucial to promoting citrus planting. A pillar industry has very important and far-reaching significance

Method used

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  • Vibrio diazotrophicus strain for resisting citruscanker
  • Vibrio diazotrophicus strain for resisting citruscanker
  • Vibrio diazotrophicus strain for resisting citruscanker

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The preparation of embodiment 1 bacterial strain T24 of the present invention

[0035] Soil samples were collected from Longshan County, Xiangxi Autonomous Prefecture. 10g of each soil sample was added to a triangular flask filled with 90ml of sterile water (with glass beads), shaken for 1h, filtered to remove sediment, and the bacterial suspension was diluted to 10% in turn. -2 、10 -3 、10 -4 、10 -5 、10 -6 . Bacteria were separated by mixing method, and fungi and actinomycetes were separated by smear method. Cultivate in LB liquid medium at 28°C for 48 hours, select colonies with different shapes, sizes, and colors, purify them, and store them in slant medium.

[0036] The citrus canker sore bacteria was mixed with the melted LB solid medium, then poured into a sterilized petri dish, and a hole was punched on the solidified gel plate, and the isolated strains were prepared into a certain concentration of bacterial strains. For the suspension, use a pipette gun to ...

Embodiment 2

[0037] Example 2 Indoor inhibitory effect of bacterial strain T24 of the present invention on canker sore citrus

[0038] Mix the bacteria solution made by X. citrus bacteria with the melted LB solid medium, pour it into a sterilized petri dish, make a hole on the solidified gel plate, and make the isolated T24 strains into a certain concentration For the bacterial suspension, use a pipette gun to take 100ul of the bacterial liquid and add it to the round hole of the corresponding plate, and set 3 parallels. Place the marked Petri dish in a 30°C incubator, and observe the formation of the inhibition zone every 24 hours. The average value of the diameter of the antibacterial zone formed as a result is about 45mm, and the edge of the antibacterial zone is slightly raised, see image 3 .

Embodiment 3

[0039] Example 3 Bacterial strain T24 of the present invention is used for preventing and treating canker sores of citrus on citrus living bodies

[0040] The bacterial strain T24 of the present invention was picked and inoculated into 25ml LB liquid medium, cultured at 37°C and 180r / min for 24h to obtain a fermentation broth. Select 3 citrus trees with similar growth conditions, select four fully expanded new leaves with similar growth and development levels on each tree, and puncture the upper epidermis of the leaves with a sterilized inoculation needle (20 holes per leaf). Spray X. citrus bacteria on the upper surface and the lower surface of the blade by spraying. After 1 d, the fermented liquid of the bacterial strain T24 of the present invention is sprayed on 3 new leaves of citrus in each treatment group processed by X. citrus as processing. , remaining 1 leaf is contrast, does not spray the fermented liquid of bacterial strain of the present invention on the contrasted b...

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Abstract

The invention relates to a vibrio diazotrophicus T24 strain for resisting citruscranker. The vibrio diazotrophicus T24 strain is already preserved China Center for Type Culture Collection on April 10, 2015, and the preservation number is CCTCC NO.M2015221. The fermentation liquid of the strain T24 has an effect for inhibiting the citruscanker, and the in-vitro inhibition rate of the sterile fermentation liquid of the strain T24 for the citruscanker reaches 90 percent or more by virtue of the test of a needle puncture method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a Vibrio diazotrophicus strain resistant to canker sores of citrus, in particular to a Vibrio diazotrophicus T24 strain, and also relates to the use of the strain T24 in preventing and treating canker citrus Applications. Background technique [0002] Citrus is a subtropical evergreen fruit tree species. Its output and planting area rank first among the fruits in the world. It is distributed in some tropical and subtropical countries and regions with suitable climates. From the perspective of citrus production, the northern hemisphere accounts for almost half of the output, while the southern hemisphere accounts for about 32% of the supply, and the rest comes from the Mediterranean coast. In tropical and subtropical regions, developing countries account for 65% of the total. In 2007, under the leadership of some countries such as Brazil and the United States, the global citrus produc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/63
Inventor 唐前君周志成肖启明戴良英李迅刘双清李魏
Owner HUNAN AGRICULTURAL UNIV