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A strain of algae scsio X and its application

A technology of SCSIOX and mat algae, applied in the direction of organic fertilizers, microorganisms, bacteria, etc., to achieve high value, promote germination, and broad application prospects

Active Publication Date: 2018-06-19
HAINAN SANYOU OCEAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] De first used nitrogen-fixing cyanobacteria as a fertilizer in paddy rice in 1939. After that, more than 10 countries including the United States, Britain, Japan and Egypt also carried out related research. In my country, Professor Li Shanghao and others first started research in this area in 1959. It has been confirmed that the inoculation of nitrogen-fixing cyanobacteria in paddy fields can effectively provide nitrogen fertilizer and fertilize the soil for rice expenditure, and increase rice yield by 10%-30%; the application of nitrogen-fixing cyanobacteria can effectively promote the growth of wheat roots and shoots and the field emergence rate (Wang Shaomei et al. ,1991), but there are few reports about the application of nitrogen-fixing cyanobacteria as bacterial fertilizer in marine ecosystems

Method used

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  • A strain of algae scsio X and its application
  • A strain of algae scsio X and its application
  • A strain of algae scsio X and its application

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0020] Example 1: Isolation, purification and identification of Phormidium sp. SCSIO X

[0021] 1. Separation and purification of Phormidium sp. SCSIO X

[0022] Collect the seaweed Thalias spp. collected from Sanya Bay, Hainan Province, China, add seawater to scrub, and filter to obtain the cyanobacteria liquid. Pick cyanobacteria hyphae and culture them in marine cyanobacteria medium ATCC Medium 957, nitrogen-fixing cyanobacteria medium ATCC Medium 819, marine nitrogen-fixing cyanobacteria medium ATCC Medium 1077 and BG-11Medium, and the continuous light intensity is 150μE / m 2 / s, the light cycle is 12:12h (light: dark cycle), the temperature is 23-27°C, in the light incubator (the model is LRH-250-GH microcomputer-controlled light incubator), every two weeks into fresh medium until the microscopic examination shows a single mycelium. The cyanobacteria SCSIO X was obtained by separation and purification.

[0023] 2. Identification of Phormidium sp. SCSIO X

[0024] 1) Mo...

Embodiment 2

[0037] The mensuration of embodiment 2 cyanobacteria indole acetic acid production

[0038] The salkowski colorimetric method was used to determine the ability of the isolate to produce plant growth hormone substances, and the standard curve was prepared using pure 3-IAA (3-indole acetic acid). Inoculate the cyanobacteria SCSIO X in 100mL selective liquid nitrogen-free liquid improved medium (formulation is the same as in Example 1), and the continuous light intensity is 150 μE / m 2 / s, the light cycle is 12:12h (light: dark cycle), the temperature is 25-28°C, cultivate for 120h, take 5mL of the cyanobacteria culture supernatant every 24h, centrifuge at 10000r / min for 10min, take the supernatant and add colorimetric The solution was left to stand in the dark for 0.5h, taken out, and measured immediately with a spectrophotometer at a wavelength of 530nm (Glickmann and Dessaux Y.A).

[0039] Indole acetic acid content in the sample (μg / mg) = (A × V1) / (W × V2) × 1000

[0040] ...

Embodiment 3

[0045] 16SRNA and nitrogen fixation gene amplification of the strain of embodiment 3

[0046] Select the pure bacterial strain cyanobacteria SCSIO X obtained by separation and purification in Example 1 to carry out DNA extraction and purification, the extraction and purification of DNA in this embodiment, its operation method refers to Dong Xiuzhu's "Common Bacteria System Identification Manual" P 409 . 1SF / 16SR (Weisenburg et al., 1991, 16S ribosomal DNA amplification forphylogenetic study., Journal of bacteriology, 1991, 173(2): 697-703) and ITS amplification primer 23S30R / 27F1 (Taton et al. ., 2003, Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): amorphological and molecular approach. Appl Environ Microb, 2003, 69:5157–5169) and nifH gene general primer PolF / PolR (reference Poly et al., 2001, Comparison of nifH Gene Poolsin Soils and Soil Microenvironments with Contrasting Properties, Applied and Environm...

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Abstract

The invention discloses a strain of algae SCSIO X and its application. Phormidium sp. SCSIO X, preservation number: CCTCC NO: M 2015307, a cyanobacteria isolated and screened from the rhizosphere of the seaweed Thalassia hemperichii that appeared later in the degraded coral reef ecosystem of Sanya Bay in the present invention A new species, Phormidium sp. SCSIO X, has good nitrogen fixation activity and can secrete 3-indole acetic acid, so the ocean-sourced Phormidium sp. SCSIO X has the ability to promote the growth of marine wetland plants It has high value and broad application prospects in the preparation of marine microbial fertility and the restoration of marine ecological environment.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, in particular a strain of algae SCSIO X and its application. Background technique: [0002] Phytohormones play important roles in plant growth and development (Sergeeva et al. 2002, 2007; Prasanna et al., 2010). It has been widely reported that microorganisms can promote plant growth, such as fungi, actinomycetes, bacteria, and cyanobacteria, among which cyanobacteria can produce a series of active metabolites such as plant growth regulators, amino acids, and polysaccharides to regulate plant growth. Growth (Ahmed et al., 2010; Mandal et al., 2011). Cyanobacteria are photoautotrophic prokaryotes that can produce vitamins, enzymes, and drugs, so they have the potential to be widely used in marine aquaculture, food, fuel, fertilizer, and colorants. In addition, because cyanobacteria can perform photosynthesis and most of them can also carry out biological nitrogen fixation, which plays such an impor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12P17/10C12R1/01
Inventor 凌娟张燕英董俊德杨清松张渊洲曾思泉江玉凤
Owner HAINAN SANYOU OCEAN TECH CO LTD
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