Gold nanoparticle growth based method for detection of glucose
A technology of gold nanoparticles and glucose, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc., which can solve the problem of expensive peroxidase, high cost, and environmental influence on activity Large and other problems, to achieve the effect of easy implementation and lower detection cost
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[0031] A method for detecting glucose based on gold nano growth, comprising the steps of:
[0032] 1) In a phosphate buffer solution with a concentration of 0.01M and a pH of 7.4, mix a group of glucose solutions of a specific concentration with glucose oxidase (GOx) respectively, and incubate at room temperature for 10 minutes to obtain a group of incubation solution a, a specific concentration of The concentration of glucose solution is 0, 0.1, 0.3, 0.5, 0.7, 0.9, 1.0mM respectively;
[0033] 2) Add 5nm gold nanoparticles and chloroauric acid to the above incubation solutions respectively, the concentration of 5nm gold nanoparticles in the incubation solution b is 8.3nM, the concentration of chloroauric acid is 0.16mM, and incubate at room temperature for 30min to obtain a set of For incubation solution b, take photos of incubation solution b and read their corresponding absorbance values at 530nm with a Synergy 2 microplate reader, and fit the standard curve according to ...
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