Method for extracting tiliroside from Tibetan drug scindapsus aureus

An extraction method, the technology of silver lintin, is applied in the field of extraction and purification of active ingredients of traditional Chinese medicine, which can solve the problems of cumbersome process steps, easy clogging of ultrafiltration membranes, and difficulty in expanding production, and achieve simple, effective and strong practicability in the extraction process route and operability effects

Inactive Publication Date: 2015-10-28
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature and patents report the extraction method of tidalin, and the patent (CN102311467A) reports a new method for separating high-purity tidalin from A. chinensis, using ultrasonic extraction, ultrafiltration, macroporous resin chromatography, and high-speed countercurrent chromatography. Separation and purification to obtain 97% silver lintin, the process steps of this method are cumbersome, the ultrafiltration membrane used in the main process section is easy to block, the cost is high, the high-speed countercurrent chromatography equipment is huge and complicated, the solvent system is difficult to choose, easy to emulsify, and the solvent consumption is large. The separation time is long, and for the complex system of natural products, high-speed countercurrent chromatography may not be able to meet the requirements of conventional production; the patent (CN103694293A) reports an extraction and purification method and detection method of linden glucoside, using 60% ethanol extraction, macroporous Resin chromatography, silica gel column chromatography, polyamide powder chromatography, ODS chromatography, MCI-gel column chromatography and other methods finally separated and obtained 96.14% silver tidalin. Expansion of production, and chromatography materials can absorb raw materials, especially silica gel materials can reach 50% of the adsorption capacity, so many chromatography methods will inevitably lead to a significant increase in production costs, which do not have development value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: The extraction method of tibetan glucoside in this Tibetan medicine green dill flower, the concrete operation is as follows:

[0022] (1) extract

[0023] Take dried 5.0 kg of green dill flowers (particle size is 1 mm) and 40 kg of ethanol solution (mass percentage concentration is 70%) and place them in a heat reflux extraction tank, reflux extraction at 50 ° C for 3 times, filter, and concentrate the filtrate to obtain a crude extract ( 613.56 g), the crude extract was dispersed with 1.5 kg hot water (45°C), cooled to room temperature and extracted twice with chloroform, the extracted part of chloroform was used for later use, and the remaining aqueous phase was further extracted three times with n-butanol; concentrated n-butanol Extract the part until the solid-liquid ratio is 1:2, cool, and filter with diatomaceous earth to obtain filtrate A;

[0024] (2) Polyamide resin adsorption and elution

[0025] Polyamide resin (GR, CXSZ-60) and filtrate A were...

Embodiment 2

[0034] Example 2: The extraction method of tibetan glucoside in this Tibetan medicine green dill flower, the concrete operation is as follows:

[0035] (1) Take 5.0 kg of dried green dill flowers (particle size is 2mm) and 45 kg of ethanol solution (65% concentration by mass percentage) and place them in a heat reflux extraction tank, reflux extraction twice at 55°C, filter, and concentrate the filtrate to obtain crude Extract (638.13 g), the crude extract was dispersed with 2.0kg of hot water (50°C), cooled to room temperature and extracted with chloroform three times, the chloroform extracted part was used for later use, and the remaining aqueous phase was further extracted twice with n-butanol; Concentrate the n-butanol extract under reduced pressure to a solid-to-liquid ratio of 1:3, cool, and filter through diatomaceous earth to obtain filtrate A;

[0036] (2) Polyamide phenol material (GR, CXSZ-60) and filtrate A are mixed in a ratio of 1L: 50g, the n-butanol solvent i...

Embodiment 3

[0040] Example 3: The extraction method of tibetan glucoside in this Tibetan medicine green dill flower, the concrete operation is as follows:

[0041] (1) Take dried 5.0 kg of green dill flowers (particle size: 3 mm) and 50 kg of ethanol solution (mass percentage concentration: 70%) in a heat reflux extraction tank, reflux extraction at 60°C for 3 times, filter, and concentrate the filtrate to obtain a crude extract (667.89 g), the crude extract was dispersed in 1.5 kg of hot water (45°C), cooled to room temperature and extracted twice with chloroform, the extracted part of chloroform was used for later use, and the remaining aqueous phase was further extracted three times with n-butanol, and concentrated under reduced pressure. Butanol extracted part until the solid-to-liquid ratio is 1:4, cooled, filtered with diatomaceous earth to obtain filtrate A;

[0042] (2) Polyamide phenol (GR, CXSZ-60) and filtrate A are mixed at a ratio of 1L: 70g, vacuum-drained n-butanol and th...

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PUM

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Abstract

The invention discloses a method for extracting tiliroside from Tibetan drug scindapsus aureus for the first time. Thermal reflux tank extraction, dry-method polyamide resin chromatography and crystallization are adopted to obtain high-purity tiliroside, and the separation method is simple and convenient; meanwhile, settling aids agents are utilized to recycle the hydrous tiliroside or the tiliroside encapsulated with surface active substances in a mother solution; the separated tiliroside and the recycled tiliroside are mixed, recrystallization is further conducted, and the purity of the finally obtained tiliroside is over 90%. The method for extracting the tiliroside from the Tibetan drug scindapsus aureus is high in extraction efficiency, low in equipment requirement and suitable for industrial production.

Description

technical field [0001] The invention belongs to a method for extracting and purifying active ingredients of traditional Chinese medicines, and in particular relates to a new method for extracting lintinin from Tibetan medicine green dill flowers. Background technique [0002] Tibetan medicine green radish ( Scindapsus aureus ) is a plant of the genus Aroma in the family Daphneaceae. Its flower buds are like pine cones, and the surface is covered with a layer of light green hairs. It is a unique and precious herbal medicinal material in the alpine region of the Himalayas. It is slightly cold in nature. Its flower buds are dried in the shade and soaked in water. Heart disease, high blood pressure and various vascular inflammation and other diseases. In 2011, Tai Zhigang et al. isolated flavonoids, triterpenes and nucleosides from the pothos flower, among which, it has been proved that there is a large amount of silver satin in the pothos flower ( Doctoral dissertation of Yu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 太志刚蔡乐丁中涛杨亚滨
Owner KUNMING UNIV OF SCI & TECH
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