Bacillus simplex and application thereof

A technology of simple bacillus and strains, applied in the field of biological control preparations, can solve the problems of long breeding cycle of disease-resistant varieties, pesticide residues, environmental pollution, etc., and achieve good application prospects, efficient control, and increase crop yield.

Active Publication Date: 2015-10-28
潍坊市信得生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of disease-resistant varieties is long and the resistance is single. However, chemical control can easily cause pesticide residues and environmental po

Method used

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  • Bacillus simplex and application thereof
  • Bacillus simplex and application thereof
  • Bacillus simplex and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Isolation and purification of Bacillus simplex strain STY005

[0025] Simple bacillus simplex (Bacillus simplex) bacterial strain STY005 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from soil, and separation method is:

[0026] (1) Isolation of Bacillus: Soil samples were collected in the nursery of Shandong Academy of Forestry Sciences, using the 5-point sampling method to collect an appropriate amount of soil within the range of 10-20cm around and in the center of the plot, and mix them in equal amounts. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies o...

Embodiment 2

[0041] 1. Fermentation process of Bacillus simplex strain STY005

[0042] LB liquid medium: the formula is 10g of tryptone, 5g of yeast extract, 10g of sodium chloride, 1000mL of distilled water, and the pH is adjusted to 7.1-7.4.

[0043] A large amount of solid fermentation medium formula (mass percentage):

[0044] Solid material: 75% cornmeal, 10% soybean meal, 15% bran;

[0045] Inorganic salt solution: potassium dihydrogen phosphate 3.5%, magnesium sulfate 0.04%, ammonium sulfate 4%, the rest is water;

[0046] The solid-to-liquid ratio is 1:1.8 (mass ratio).

[0047] Bacillus simplex (B.simplex) STY005 mass solid fermentation process:

[0048] ①Cultivation of strains in seed solution: Pick a small amount of bacteria from the slant of the test tube of Bacillus simplex STY005, transfer it to LB liquid medium, shake and culture at 30°C for 3-5 days, and this is the seed solution;

[0049] ②Cultivation of solid production strains: Inoculate the seed liquid into solid me...

Embodiment 3

[0051] Embodiment 3: Control test of potato scab

[0052] This example provides related experiments on the control effect of the original powder of Bacillus simplex strain STY005 on potato scab.

[0053] 1) Test drug

[0054] The original powder of Bacillus simplex strain STY005, prepared in Example 2;

[0055] 2) Test crops and control objects

[0056] Test crop: potato; variety: Holland 15; control object: potato scab;

[0057] 3) Test process

[0058] Test location: Anzhuang Town, Feicheng City, Shandong Province.

[0059] In this experiment, the original powder of simple Bacillus strain STY005 was diluted 200 times, 400 times, 600 times and 800 times, and clear water was used as a control for a total of 5 treatments. On June 5, 2014, root irrigation was adopted for prevention and control, and the area of ​​the plot was 50m 2 , repeated 4 times. Dilute the test agent with water 1000 times, unscrew the nozzle of the sprayer, and spray along the potato plants, every 66...

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Abstract

The invention discloses a strain of bacillus simplex and application thereof. The bacterial strain is bacillus simplex STY005, and the preservation number is CGMCC No.11052. The invention also discloses a biological control preparation containing the bacterial strain and a preparation method thereof. Experiment results show that the bacillus simplex strain STY005 provided by the invention has the characteristics of being long in diameter, hypoxia resistant, wide in action spectrum, high in adaptability, capable of quickly growing and breeding under low nutrition, and the like, thereby having a good application prospect; the biological control preparation prepared from the bacterial strain can not only effectively prevent and treat plant diseases, but also effectively increase crop yield, is a biological control preparation with a wide application prospect, and can be used as a biopesticide or a bio-fertilizer for preventing and treating crop leaf diseases containing one or more of the diseases like potato common scab, grape downy mildew, Chinese rose powdery mildew, and cucumber target leaf spot.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a bacillus capable of efficiently preventing and controlling plant diseases and its use, and a biological control agent prepared by using the bacillus. Background technique [0002] Plant disease is a phenomenon in which plants undergo a series of morphological, physiological and biochemical pathological changes under the influence of biological or abiotic factors, hindering the normal growth and development process, thereby affecting human economic benefits. At present, for the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties and biological control. The breeding cycle of disease-resistant varieties is long and the resistance is single. Chemical control can easily cause pesticide residues and environmental pollution. At the same time, the l...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 戴宝牛赡光刘幸红王清海卢德鹏朱福庆王栋梁马瑞滨孔雪华李秀香
Owner 潍坊市信得生物科技有限公司
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