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Breeding method of few-seed watermelons

A watermelon and seed technology, which is applied in the field of seedless watermelon breeding, can solve problems such as the cultivation of translocation lines with unfavorable and good traits, watermelon gene mutation, etc., and achieve the effect of simple method, easy operation, and stable seedless traits

Inactive Publication Date: 2015-11-04
ZHENJIANG SUIHAN AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is feasible to use irradiation to induce translocation of multiple pairs of chromosomes to breed translocation lines with few seeds, but irradiation treatment will inevitably cause mutations in watermelon genes, which is not conducive to the cultivation of translocation lines with excellent traits.

Method used

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  • Breeding method of few-seed watermelons
  • Breeding method of few-seed watermelons
  • Breeding method of few-seed watermelons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Breeding of a few-seed watermelon line W1301:

[0020] Step 1. Select the watermelon variety "Jingxin No. 1" and self-bred for 5 consecutive generations to obtain inbred lines wmj08, wmj09 and wmj10 with different traits.

[0021] Step 2: Soak the seeds of the inbred line wmj10 with distilled water until they are saturated with water, then transfer them to 1.3% EMS phosphate buffer (phosphate buffer concentration is 0.05mol / L, PH=7.0) for 8h, and rinse with water after treatment 3 times, then rinse with running water for 30 minutes. After sowing the seeds (E1), 150 plants were sown, numbered A1-A150 respectively. After flowering, the pollen viability identification of each line was carried out. A total of 7 individual plants with pollen viability less than 50% were identified, numbered B1-B7, and then artificially bagged and selfed B1-B7;

[0022] Step 3: Sow selfed seeds (E2) of B1~B7, and sown 20 plants per plant. To E 2 The pollen viability identification of ea...

Embodiment 2

[0031] Example 2 Breeding of low-seed watermelon line W1406:

[0032] Step 1. Select the watermelon variety ‘Xiaolan’ and self-bred for 5 consecutive generations to obtain inbred lines wmx03, wmx06 and wmx08 with different traits.

[0033] Step 2. Soak the seeds of the inbred line wmx08 with distilled water until they are saturated with water, then transfer them to 1.3% EMS phosphate buffer (phosphate buffer concentration is 0.05mol / L, PH=7.0) for 8h, and rinse with water after treatment 3 times, then rinse with running water for 30 minutes. After sowing the seeds (E1), 150 plants were sown, numbered A1-A150 respectively. After flowering, the pollen viability identification of each line was carried out. A total of 4 individual plants with pollen viability less than 50% were identified, numbered B1~B4, and then B1~B4 were artificially bagged and selfed;

[0034] Step 3: Sow selfed seeds (E2) from B1 to B4, and sown 20 plants per plant. To E 2 The pollen viability identification of ...

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Abstract

The invention discloses a breeding method of few-seed watermelons. Watermelon selfing line seeds E1 are soaked in distilled water and then put into an EMS phosphate buffer to be processed, and the processed watermelon selfing line seeds are sown; after flowering, pollen vitality identification is conducted on all strains, and selfing fertilization is conducted on the single plants with the pollen vitality lower than 50 percent; selfing seeds harvested from the single plants are sown, and 20 plants are sown for each single plant; strains with normal pollen fertility are selected as male parents, the non-processed watermelon selfing line serves as female parents, common hybridization is conducted, selfing fertilization is conducted on the male parents, and the male parent seeds and the hybrid seeds are collected; the hybrid seeds are sown, male parents with low pollen vitality of all single plants in the same combination are marked, the marked male parents and other watermelon selfing lines are subjected to hybridization, and the first-filial generation combination with few seeds is selected. The few-seed watermelonss obtained through the method can keep the superior properties of the original selfing line, and the first-filial generation shows the stable few-seed property.

Description

Technical field [0001] The invention relates to a breeding method for a few-seed watermelon, belonging to the technical field of plant breeding. Background technique [0002] Watermelon [Citrullus lanatus (Thunb.) Matsum et Nakai] is an important horticultural crop, and seedless or seedless watermelon is an important indicator of high-quality watermelon. Few-seed watermelon can be divided into: hormone less seed, polyploid less seed and translocation less seed. The current multiploid seed method has the fatal weakness of low seed collection, low germination rate and low seedling rate. Translocated few-seed watermelon is a diploid hybrid first-generation watermelon variety prepared by using chromosomal translocation line few-seed parents. The few-seed watermelon obtained by this method can take into account many advantages of ordinary diploid varieties, and it is important to show stable few-seed traits. Therefore, breeding new watermelon lines with few seeds and high quality th...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02
Inventor 孙春青潘跃平马志虎戴忠良毛忠良吴国平秦文斌姚悦梅张振超潘永飞孙国胜王建华陈智超
Owner ZHENJIANG SUIHAN AGRI