Anthurium rapid propagation method
An anthurium and fast technology, applied in the field of rapid propagation of anthurium, can solve the problems of high price, easy browning of anthurium, pollution, etc., and achieve the effects of improving survival, shortening tissue culture time, and reducing costs.
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Embodiment 1
[0023] Induction medium: MS, KT 3 mg / L, NAA 0.5mg / L, IAA 0.2 mg / L activated carbon 2g / L, sucrose 25g / L, agar 6g / L.pH=5.6;
[0024] Proliferation medium: MS, 6-BA 0.7mg / L, NAA3mg / L, sucrose 30g / L, agar 7g / L.pH=5.6;
[0025] Rooting medium: 1 / 2MS, NAA0.2mg / L, sucrose 15g / L, agar 6.5 g / L, pH=5.6-5.8;
[0026] Anthurium stem tips were selected as explants, soaked in 3% ascorbic acid for 10 min, rinsed in sterile water three times, soaked in 75% ethanol for 8 seconds, rinsed in sterile water three times, rinsed in 0.1% HgCl 2 Soak for 8min, rinse with sterile water to remove HgCl 2 , inoculate it in the induction medium under a sterile environment and cultivate it in the dark for 5 days first, then culture it under light, the light time is 8h / d, the light intensity is 1000lx, the culture temperature is 24°C, and after 20 days of culture, the shoot tip begins to produce yellow-green Callus, the callus induction rate reached 98%, transferred to the proliferation medium, the light t...
Embodiment 2
[0028] Induction medium: MS, 6-BA 5 mg / L, 2,4-D 1 mg / L, activated carbon 2 g / L, sucrose 28 g / L, agar 6 g / L, pH=5.6;
[0029] Proliferation medium: MS, 6-BA 0.5 mg / L, NAA 3.5 mg / L, sucrose 30g / L, agar 5.8 g / L, pH=5.6;
[0030] Rooting medium: 1 / 2MS, NAA 0.6 mg / L, sucrose 20 g / L, agar 6 g / L, pH=5.6-5.8;
[0031] Anthurium stem tips were selected as explants, soaked in 3% polyvinylpyrrolidone for 10 min, rinsed with sterile water three times, soaked in 75% ethanol for 5 seconds, rinsed with sterile water three times, and rinsed in 0.4% HgCl 2 Soak for 8min, rinse with sterile water to remove HgCl 2 , inoculate it in the induction medium under a sterile environment and cultivate it in the dark for 10 days, and then cultivate it in the light. Green callus, the callus induction rate reached 96%, transferred to the proliferation medium, light 10h / d, light intensity 3300lx, cultured at 26°C for 20d, an average of 7 adventitious buds per piece of callus, waiting for adventitious bud ...
Embodiment 3
[0033] Induction medium: MS, 6-BA 3mg / L, 2, 4-D 0.5mg / L, NAA 0.5mg / L, activated carbon 5 g / L, sucrose 30 g / L, agar 6 g / L.pH=5.8 ;
[0034] Proliferation medium: MS, 6-BA 0.5mg / L, NAA 4 mg / L, sucrose 28g / L, agar 6 g / L, pH=5.6;
[0035] Rooting medium: 1 / 2MS, IAA 0.8mg / L, sucrose 25g / L, agar 6.5 g / L, pH=5.6-5.8;
[0036] Anthurium stem tips were selected as explants, soaked in 2% ascorbic acid for 20 min, rinsed with sterile water three times, soaked in 75% ethanol for 8 seconds, rinsed with sterile water three times, rinsed in 0.1% HgCl 2 Soak for 8min, rinse with sterile water to remove HgCl 2 , inoculate it in the induction medium under a sterile environment and cultivate it in the dark for 10 days, and then cultivate it in the light, the light time is 8h / d, the light intensity is 800 lx, the culture temperature is 24°C, and after 15 days of culture, the shoot tip begins to produce Yellow-green callus, the callus induction rate reached 96%, transferred to the proliferation...
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