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Anthurium rapid propagation method

An anthurium and fast technology, applied in the field of rapid propagation of anthurium, can solve the problems of high price, easy browning of anthurium, pollution, etc., and achieve the effects of improving survival, shortening tissue culture time, and reducing costs.

Inactive Publication Date: 2015-11-04
虞龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been loved by the majority of people, but its core technology has been monopolized by foreign countries, so the price is quite expensive
With the improvement of people's living standards, the demand for anthurium is also increasing. Many domestic flower companies have turned their attention to the production of finished anthurium. Only relying on imported seedlings can no longer meet the needs of the domestic market. Anthurium Like other fashionable flowers, changing its flower shape and color, shortening the growth cycle, and enhancing variety resistance have become the main goals of anthurium breeding
[0003] my country has made some progress in the research on anthurium tissue culture, but anthurium is easy to brown and pollute after inoculation of explants, the induction rate of explants is low, it is not easy to take root, and in the culture conditions, hormone ratio There are quite different opinions on other aspects, so it is necessary to explore a method suitable for the rapid propagation of anthurium tissue

Method used

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  • Anthurium rapid propagation method

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Experimental program
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Effect test

Embodiment 1

[0023] Induction medium: MS, KT 3 mg / L, NAA 0.5mg / L, IAA 0.2 mg / L activated carbon 2g / L, sucrose 25g / L, agar 6g / L.pH=5.6;

[0024] Proliferation medium: MS, 6-BA 0.7mg / L, NAA3mg / L, sucrose 30g / L, agar 7g / L.pH=5.6;

[0025] Rooting medium: 1 / 2MS, NAA0.2mg / L, sucrose 15g / L, agar 6.5 g / L, pH=5.6-5.8;

[0026] Anthurium stem tips were selected as explants, soaked in 3% ascorbic acid for 10 min, rinsed in sterile water three times, soaked in 75% ethanol for 8 seconds, rinsed in sterile water three times, rinsed in 0.1% HgCl 2 Soak for 8min, rinse with sterile water to remove HgCl 2 , inoculate it in the induction medium under a sterile environment and cultivate it in the dark for 5 days first, then culture it under light, the light time is 8h / d, the light intensity is 1000lx, the culture temperature is 24°C, and after 20 days of culture, the shoot tip begins to produce yellow-green Callus, the callus induction rate reached 98%, transferred to the proliferation medium, the light t...

Embodiment 2

[0028] Induction medium: MS, 6-BA 5 mg / L, 2,4-D 1 mg / L, activated carbon 2 g / L, sucrose 28 g / L, agar 6 g / L, pH=5.6;

[0029] Proliferation medium: MS, 6-BA 0.5 mg / L, NAA 3.5 mg / L, sucrose 30g / L, agar 5.8 g / L, pH=5.6;

[0030] Rooting medium: 1 / 2MS, NAA 0.6 mg / L, sucrose 20 g / L, agar 6 g / L, pH=5.6-5.8;

[0031] Anthurium stem tips were selected as explants, soaked in 3% polyvinylpyrrolidone for 10 min, rinsed with sterile water three times, soaked in 75% ethanol for 5 seconds, rinsed with sterile water three times, and rinsed in 0.4% HgCl 2 Soak for 8min, rinse with sterile water to remove HgCl 2 , inoculate it in the induction medium under a sterile environment and cultivate it in the dark for 10 days, and then cultivate it in the light. Green callus, the callus induction rate reached 96%, transferred to the proliferation medium, light 10h / d, light intensity 3300lx, cultured at 26°C for 20d, an average of 7 adventitious buds per piece of callus, waiting for adventitious bud ...

Embodiment 3

[0033] Induction medium: MS, 6-BA 3mg / L, 2, 4-D 0.5mg / L, NAA 0.5mg / L, activated carbon 5 g / L, sucrose 30 g / L, agar 6 g / L.pH=5.8 ;

[0034] Proliferation medium: MS, 6-BA 0.5mg / L, NAA 4 mg / L, sucrose 28g / L, agar 6 g / L, pH=5.6;

[0035] Rooting medium: 1 / 2MS, IAA 0.8mg / L, sucrose 25g / L, agar 6.5 g / L, pH=5.6-5.8;

[0036] Anthurium stem tips were selected as explants, soaked in 2% ascorbic acid for 20 min, rinsed with sterile water three times, soaked in 75% ethanol for 8 seconds, rinsed with sterile water three times, rinsed in 0.1% HgCl 2 Soak for 8min, rinse with sterile water to remove HgCl 2 , inoculate it in the induction medium under a sterile environment and cultivate it in the dark for 10 days, and then cultivate it in the light, the light time is 8h / d, the light intensity is 800 lx, the culture temperature is 24°C, and after 15 days of culture, the shoot tip begins to produce Yellow-green callus, the callus induction rate reached 96%, transferred to the proliferation...

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Abstract

The invention discloses an anthurium rapid propagation method. According to the method, anthurium ramets are selected, and are soaked for 10-30min in an antioxidant with a mass percentage of 1-5%; after the sterilization disinfection treatment, the ramets are inoculated into an induction medium for culturing; dark culture is carried out for 5-10 days, and light culture is carried out, such that anthurium callus are obtained; the callus are transferred to a proliferation medium, and are cultured for 20-25 days with an illumination time of 10-12h / d, an illumination intensity of 3000-3500 lx and a culture temperature of 22-28 DEG C, such that buds are differentiated; when the differentiated buds are 1-2cm, the buds are transferred into a rooting medium, and are cultured for 20-30 days with an illumination time of 5-8h / d, an illumination intensity of 1500-2000 lx and a culture temperature of 22-28 DEG C, such that rooted anthurium is obtained. With the method, a large amount of anthurium with good genetic stability can be obtained rapidly; a tissue culture cycle is shortened; anthurium differentiation rate and rooting rate are greatly improved, and anthurium tissue culture cost is saved.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for rapid propagation of anthurium. Background technique [0002] Anthurium, also known as anthurium, anthurium, is a perennial epiphytic evergreen herb of the family Araceae Anthurium, native to the tropical rain forests of South America. In recent years, the quality of anthurium has been getting higher and higher, the domestic market is relatively stable, the market acceptance is getting better and better, the sales are stable, and the prospect is promising. Since 1980, tissue culture technology has developed rapidly. Since the introduction of anthurium in my country in the 1980s, due to its unique flower shape and unique leaf shape, it has been endowed with the meaning of "great ambitions and enthusiasm". It has been loved by the people, but its core technology has been monopolized by foreign countries, so the price is quite expensive. With th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 虞龙虞沐尧
Owner 虞龙
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