A method for identifying high-yield traits of royal jelly in bee colonies using the snp marker rs5749071
A bee colony royal jelly, high-yield technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of unfavorable popularization, complicated identification process, high cost, etc., and achieve the goal of improving success rate, rapid identification and improving efficiency Effect
Inactive Publication Date: 2018-10-23
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology
The more reliable ones are the three molecular markers screened by Pan Jiao et al. (2012) using gene chip technology within the range of the honey bee genome. However, this method requires the extraction of honey bee RNA and the use of fluorescent quantitative instruments, which makes the identification process complicated and expensive. , which is not conducive to the popularization of the method
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Embodiment 1
[0037] A method for identifying high-yield traits of honey bee colony royal jelly using SNP marker rs5749071, comprising the following steps:
[0038] (1) Sampling of honeybees: 50 adult worker bee individuals were taken from the bee species with high royal jelly production ("Zhenongda No. 1") and the bee species with low royal jelly production (Apis mellifera, USA), and each individual was placed in a centrifuge tube, and then Freeze at -20°C for later use;
[0039] (2) Extraction of honeybee sample DNA (using Sangon’s animal genome rapid extraction kit, SK8222)
[0040] ①Use ophthalmic scissors to cut off the limbs, wings and abdomen of the individual worker bees in step (1), keep only the head and chest, put them in a mortar filled with liquid nitrogen, crush them with a grinding rod, and then transfer them to a 1.5mL centrifuge. Add 400 μL BufferDigestion to the tube, shake evenly, and bathe in water at 65°C for 1 hour until the cells are completely lysed. Then remove th...
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The invention relates to a method for identifying a high yield character of swarm royal jelly by means of an SNP marker rs5749071. The method comprises the steps of bee individual sampling, bee sample DNA extracting, primer synthesizing, PCR testing and identifying of the high yield character of the swarm royal jelly; the high yield character of the swarm royal jelly is identified according to the fact that whether a significant difference exists between the probabilities PC and PT of a C allele and a T allele, appearing in the rs5749071 marked by the SNP, of worker bee individuals collected from a swarm at random. According to the method for identifying the high yield character of the swarm royal jelly by means of the SNP marker rs5749071, a mode that the high yield performance of the swarm royal jelly of apis mellifera is scientifically, accurately and quickly identified by using the SNP (rs5749071) marker relevant to the high yield of the royal jelly of the worker bee individuals of the apis mellifera is supplied from the molecular biology level, therefore, the breeding cycle of an apis mellifera breed with the high yield of the royal jelly can be greatly shortened, and the breeding speed of the apis mellifera can be accelerated.
Description
technical field [0001] The invention relates to a method for identifying high-yield traits of bee colony royal jelly, in particular to a method for identifying high-yield traits of bee colony royal jelly by using SNP marker rs5749071, and belongs to the field of biotechnology. Background technique [0002] Royal jelly has many important nutritional and medicinal values, is very beneficial to human health, and is a natural nourishing food. my country is a big beekeeping country, and the output of royal jelly accounts for more than 90% of the world's total, which has an inseparable relationship with the bee varieties raised in my country. Our country's bee industry scientists and scientific and technical personnel have bred "Zhenongda No. 1" and Xiaoshan jellyfish through the closed breeding method of bee groups. , Has become my country's unique and main bee species. For a long time, my country's bee industry scientific and technological workers have been committed to the res...
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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 苏松坤刘小彦李志国刘元珍许叔鹏
Owner FUJIAN AGRI & FORESTRY UNIV